Literature summary extracted from
Mellroth, P.; Steiner, H.
PGRP-SB1: an N-acetylmuramoyl L-alanine amidase with antibacterial activity (2006), Biochem. Biophys. Res. Commun., 350, 994-999.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.5.1.28 |
gene PGRP-SB1, DNA and amino acid sequence determination and analysis, expression of His6-tagged 128 C-terminal residues and of his6-tagged 180 N-terminal residues in Escherichia coli strain XL-1I Blue |
Drosophila melanogaster |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.5.1.28 |
1,10-phenanthroline |
inactivation through depletion of zinc ions |
Drosophila melanogaster |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.5.1.28 |
Zn2+ |
absolutely required for activity |
Drosophila melanogaster |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.5.1.28 |
additional information |
Drosophila melanogaster |
the enzyme performs cell wall lysis, and shows antibacterial activity against Bacillus megaterium |
? |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.5.1.28 |
Drosophila melanogaster |
Q70PY2 |
gene PGRP-SB1 or CG9681; gene PGRP-SB1 or CG9681 |
- |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.5.1.28 |
S2 cell |
- |
Drosophila melanogaster |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.5.1.28 |
additional information |
the enzyme performs cell wall lysis, and shows antibacterial activity against Bacillus megaterium |
Drosophila melanogaster |
? |
- |
? |
|
3.5.1.28 |
N-acetylmuramoyl-L-alanine + H2O |
the enzyme performs cell wall lysis by cleavage of N-acetylmuramoyl-L-alanine bonds in dimeric cross-bridges that interlink the two murein strands in the peptidoglycan, peptidoglycan substrate from Escherichia coli, PGRP-SB1 is highly active against peptidoglycans that have a diaminopimelic acid residue in the cross-linking peptide, but lacks activity to most lysine-containing peptidoglycans |
Drosophila melanogaster |
N-acetylmuramate + L-alanine |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.5.1.28 |
More |
the enzyme belongs to the peptidoglycan recognition protein, PGRP, family |
Drosophila melanogaster |
3.5.1.28 |
PGRP-SB1 |
- |
Drosophila melanogaster |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.5.1.28 |
22 |
25 |
assay at |
Drosophila melanogaster |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.5.1.28 |
7.2 |
- |
assay at |
Drosophila melanogaster |