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Literature summary extracted from

  • Carroll, K.S.; Gao, H.; Chen, H.; Leary, J.A.; Bertozzi, C.R.
    Investigation of the iron-sulfur cluster in Mycobacterium tuberculosis APS reductase: implications for substrate binding and catalysis (2005), Biochemistry, 44, 14647-14657.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.99.2 expressed in Escherichia coli Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.8.99.2 C140S the mutation has a deleterious effect on the amount of iron associated with the enzyme, no detectable catalytic activity Mycobacterium tuberculosis
1.8.99.2 C141S the mutation has a deleterious effect on the amount of iron associated with the enzyme, no detectable catalytic activity Mycobacterium tuberculosis
1.8.99.2 C223S the mutation has a deleterious effect on the amount of iron associated with the enzyme, no detectable catalytic activity Mycobacterium tuberculosis
1.8.99.2 C226S the mutation has a deleterious effect on the amount of iron associated with the enzyme, no detectable catalytic activity Mycobacterium tuberculosis
1.8.99.2 C249S the mutation has a deleterious effect on the amount of iron associated with the enzyme, no detectable catalytic activity Mycobacterium tuberculosis
1.8.99.2 C59S the mutation has a deleterious effect on the amount of iron associated with the enzyme, low catalytic activity Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.99.2 28700
-
gel filtration Mycobacterium tuberculosis
1.8.99.2 28710
-
electrospray FT-ICR mass spectrometry Mycobacterium tuberculosis
1.8.99.2 600000
-
gel filtration, the majority of the protein is aggregated Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.8.99.2 Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.99.2 adenylyl sulfate + reduced thioredoxin
-
Mycobacterium tuberculosis AMP + sulfite + oxidized thioredoxin
-
?

Synonyms

EC Number Synonyms Comment Organism
1.8.99.2 adenosine 5'-phosphosulfate reductase
-
Mycobacterium tuberculosis