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Literature summary extracted from

  • Wilson, H.L.; Wilkinson, S.R.; Rajagopalan, K.V.
    The G473D mutation impairs dimerization and catalysis in human sulfite oxidase (2006), Biochemistry, 45, 2149-2160.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.2.1 expressed in Escherichia coli strain TP1000 Homo sapiens
1.8.3.1 expressed in Escherichia coli strain TP1000 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.8.2.1 G473A mutant is able to dimerize and has steady-state activity comparable to that of the wild type, stopped-flow analysis of the reductive half-reaction of this variant yields a rate constant nearly 3 times higher than that of the wild type Homo sapiens
1.8.2.1 G473D monomer, mutant is severely impaired both in the ability to bind sulfite and in catalysis, with a second-order rate constant 5 orders of magnitude lower than that of the wild type, significant random-coil formation Homo sapiens
1.8.2.1 G473W monomer, mutant with 5fold higher activity than G473D and nearly wild-type activity at pH 7.0 when ferricyanide is the electron acceptor, significant random-coil formation Homo sapiens
1.8.2.1 R212A/G473D mutant is able to oligomerize but has undetectable activity, significant random-coil formation Homo sapiens
1.8.3.1 G473A mutant is able to dimerize and has steady-state activity comparable to that of the wild type, stopped-flow analysis of the reductive half-reaction of this variant yields a rate constant nearly 3 times higher than that of the wild type Homo sapiens
1.8.3.1 G473D monomer, mutant is severely impaired both in the ability to bind sulfite and in catalysis, with a second-order rate constant 5 orders of magnitude lower than that of the wild type, significant random-coil formation Homo sapiens
1.8.3.1 G473W monomer, mutant with 5fold higher activity than G473D and nearly wild-type activity at pH 7.0 when ferricyanide is the electron acceptor, significant random-coil formation Homo sapiens
1.8.3.1 R212A/G473D mutant is able to oligomerize but has undetectable activity, significant random-coil formation Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.2.1 0.00456
-
sulfite wild type enzyme, pH 7.0, using ferricyanide as the electron acceptor Homo sapiens
1.8.2.1 0.00605
-
sulfite mutant enzyme G473A, pH 7.0, using ferricyanide as the electron acceptor Homo sapiens
1.8.2.1 0.0149
-
sulfite wild type enzyme, pH 8.6, using ferricyanide as the electron acceptor Homo sapiens
1.8.2.1 0.191
-
sulfite mutant enzyme G473A, pH 8.5, using ferricyanide as the electron acceptor Homo sapiens
1.8.2.1 21.2
-
sulfite mutant enzyme G473W, pH 7.0, using ferricyanide as the electron acceptor Homo sapiens
1.8.2.1 24
-
sulfite mutant enzyme G473W, pH 8.5, using ferricyanide as the electron acceptor Homo sapiens
1.8.2.1 26.5
-
sulfite mutant enzyme G473D, pH 7.0, using ferricyanide as the electron acceptor Homo sapiens
1.8.2.1 41.4
-
sulfite mutant enzyme G473D, pH 8.5, using ferricyanide as the electron acceptor Homo sapiens
1.8.3.1 0.00043
-
cytochrome c mutant enzyme G473A, pH 8.5 Homo sapiens
1.8.3.1 0.00082
-
cytochrome c mutant enzyme G473A, pH 7.0 Homo sapiens
1.8.3.1 0.00123
-
cytochrome c mutant enzyme G473A, pH 6.0 Homo sapiens
1.8.3.1 0.00129
-
sulfite wild type enzyme, pH 6.0 Homo sapiens
1.8.3.1 0.00141
-
cytochrome c wild type enzyme, pH 7.0 Homo sapiens
1.8.3.1 0.00162
-
sulfite wild type enzyme, pH 6.5 Homo sapiens
1.8.3.1 0.00173
-
cytochrome c wild type enzyme, pH 6.0 Homo sapiens
1.8.3.1 0.00272
-
sulfite wild type enzyme, pH 7.0 Homo sapiens
1.8.3.1 0.00339
-
sulfite wild type enzyme, pH 7.5 Homo sapiens
1.8.3.1 0.00371
-
cytochrome c mutant enzyme G473D, pH 8.6 Homo sapiens
1.8.3.1 0.00435
-
sulfite wild type enzyme, pH 8.0 Homo sapiens
1.8.3.1 0.0044
-
cytochrome c wild type enzyme, pH 8.5 Homo sapiens
1.8.3.1 0.00453
-
sulfite mutant enzyme G473A, pH 6.0 Homo sapiens
1.8.3.1 0.00474
-
cytochrome c mutant enzyme G473D, pH 8.0 Homo sapiens
1.8.3.1 0.00536
-
sulfite mutant enzyme G473A, pH 7.0 Homo sapiens
1.8.3.1 0.00536
-
cytochrome c mutant enzyme G473W, pH 8.5 Homo sapiens
1.8.3.1 0.00825
-
sulfite wild type enzyme, pH 8.5 Homo sapiens
1.8.3.1 0.014
-
cytochrome c mutant enzyme G473D, pH 7.5 Homo sapiens
1.8.3.1 0.0172
-
sulfite mutant enzyme G473A, pH 7.4 Homo sapiens
1.8.3.1 0.0221
-
sulfite wild type enzyme, pH 9.0 Homo sapiens
1.8.3.1 0.0357
-
cytochrome c mutant enzyme G473W, pH 7.0 Homo sapiens
1.8.3.1 0.0362
-
cytochrome c mutant enzyme G473D, pH 7.0 Homo sapiens
1.8.3.1 0.0487
-
sulfite mutant enzyme G473A, pH 8.0 Homo sapiens
1.8.3.1 0.0635
-
cytochrome c mutant enzyme G473D, pH 6.5 Homo sapiens
1.8.3.1 0.0671
-
sulfite wild type enzyme, pH 9.5 Homo sapiens
1.8.3.1 0.0905
-
cytochrome c mutant enzyme G473W, pH 6.0 Homo sapiens
1.8.3.1 0.107
-
sulfite mutant enzyme G473A, pH 8.5 Homo sapiens
1.8.3.1 0.107
-
cytochrome c mutant enzyme G473D, pH 6.0 Homo sapiens
1.8.3.1 0.33
-
sulfite mutant enzyme G473W, pH 7.0 Homo sapiens
1.8.3.1 0.623
-
sulfite mutant enzyme G473D, pH 7.0 Homo sapiens
1.8.3.1 0.774
-
sulfite mutant enzyme G473A, pH 9.1 Homo sapiens
1.8.3.1 0.99
-
sulfite mutant enzyme G473D, pH 6.5 Homo sapiens
1.8.3.1 1.063
-
sulfite mutant enzyme G473D, pH 7.5 Homo sapiens
1.8.3.1 1.223
-
sulfite mutant enzyme G473D, pH 8.0 Homo sapiens
1.8.3.1 1.66
-
sulfite mutant enzyme G473D, pH 6.0 Homo sapiens
1.8.3.1 1.91
-
sulfite mutant enzyme G473W, pH 6.0 Homo sapiens
1.8.3.1 2.034
-
sulfite mutant enzyme G473W, pH 8.5 Homo sapiens
1.8.3.1 2.04
-
sulfite mutant enzyme G473D, pH 8.5 Homo sapiens
1.8.3.1 3.684
-
sulfite mutant enzyme G473A, pH 10.0 Homo sapiens
1.8.3.1 10.41
-
sulfite mutant enzyme G473W, pH 9.0 Homo sapiens
1.8.3.1 25.88
-
sulfite mutant enzyme G473D, pH 9.1 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.2.1 Mo consists of a single molybdenum atom coordinated through the dithiolene group of a single molybdopterin molecule Homo sapiens
1.8.3.1 Molybdenum consists of a single molybdenum atom coordinated through the dithiolene group of a single molybdopterin molecule Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.2.1 52000
-
1 * 52000, mutant G473D, calculation from amino acid sequence Homo sapiens
1.8.2.1 52000
-
2 * 52000, wild type enzyme, sedimentation equilibrium analysis Homo sapiens
1.8.2.1 53500
-
1 * 53500, mutant G473D, gel filtration and sedimentation equilibrium analysis Homo sapiens
1.8.2.1 54500
-
1 * 54500, mutant G473W, sedimentation equilibrium analysis Homo sapiens
1.8.2.1 66600
-
1 * 66600, double mutant R212A/G473D, sedimentation equilibrium analysis Homo sapiens
1.8.2.1 101000
-
wild type enzyme, sedimentation equilibrium analysis Homo sapiens
1.8.3.1 52000
-
1 * 52000, mutant G473D, calculation from amino acid sequence Homo sapiens
1.8.3.1 52000
-
2 * 52000, wild type enzyme, sedimentation equilibrium analysis Homo sapiens
1.8.3.1 53500
-
1 * 53500, mutant G473D, gel filtration and sedimentation equilibrium analysis Homo sapiens
1.8.3.1 54500
-
1 * 54500, mutant G473W, sedimentation equilibrium analysis Homo sapiens
1.8.3.1 66600
-
1 * 66600, double mutant R212A/G473D, sedimentation equilibrium analysis Homo sapiens
1.8.3.1 101000
-
wild type enzyme, sedimentation equilibrium analysis Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.1 Homo sapiens
-
-
-
1.8.3.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.1 phenyl-Sepharose column chromatography and Superdex 200 16/60 column gel filtration Homo sapiens
1.8.3.1 phenyl-Sepharose column chromatography and Superdex 200 16/60 FPLC column gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.1 sulfite + ferricyanide + H2O
-
Homo sapiens sulfate + ferrocyanide
-
?
1.8.3.1 sulfite + cytochrome c
-
Homo sapiens sulfate + reduced cytochrome c
-
?

Subunits

EC Number Subunits Comment Organism
1.8.2.1 dimer 2 * 52000, wild type enzyme, sedimentation equilibrium analysis Homo sapiens
1.8.2.1 monomer 1 * 52000, mutant G473D, calculation from amino acid sequence Homo sapiens
1.8.2.1 monomer 1 * 53500, mutant G473D, gel filtration and sedimentation equilibrium analysis Homo sapiens
1.8.2.1 monomer 1 * 54500, mutant G473W, sedimentation equilibrium analysis Homo sapiens
1.8.2.1 monomer 1 * 66600, double mutant R212A/G473D, sedimentation equilibrium analysis Homo sapiens
1.8.3.1 dimer 2 * 52000, wild type enzyme, sedimentation equilibrium analysis Homo sapiens
1.8.3.1 monomer 1 * 52000, mutant G473D, calculation from amino acid sequence Homo sapiens
1.8.3.1 monomer 1 * 53500, mutant G473D, gel filtration and sedimentation equilibrium analysis Homo sapiens
1.8.3.1 monomer 1 * 54500, mutant G473W, sedimentation equilibrium analysis Homo sapiens
1.8.3.1 monomer 1 * 66600, double mutant R212A/G473D, sedimentation equilibrium analysis Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.8.2.1 SO
-
Homo sapiens
1.8.2.1 sulfite oxidase
-
Homo sapiens
1.8.3.1 SO
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.2.1 25
-
-
Homo sapiens
1.8.3.1 25
-
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.3.1 0.14
-
sulfite mutant enzyme G473D, pH 6.0 Homo sapiens
1.8.3.1 0.28
-
sulfite mutant enzyme G473D, pH 6.5 Homo sapiens
1.8.3.1 0.34
-
cytochrome c mutant enzyme G473D, pH 6.0 Homo sapiens
1.8.3.1 0.42
-
sulfite mutant enzyme G473D, pH 9.1 Homo sapiens
1.8.3.1 0.48
-
cytochrome c mutant enzyme G473D, pH 8.6 Homo sapiens
1.8.3.1 0.5
-
sulfite mutant enzyme G473D, pH 7.0 Homo sapiens
1.8.3.1 0.53
-
cytochrome c mutant enzyme G473D, pH 6.5 Homo sapiens
1.8.3.1 0.54
-
sulfite mutant enzyme G473D, pH 8.5 Homo sapiens
1.8.3.1 0.57
-
sulfite mutant enzyme G473D, pH 7.5 Homo sapiens
1.8.3.1 0.58
-
sulfite mutant enzyme G473D, pH 8.0 Homo sapiens
1.8.3.1 0.59
-
cytochrome c mutant enzyme G473D, pH 8.0 Homo sapiens
1.8.3.1 0.6
-
sulfite mutant enzyme G473W, pH 6.0 Homo sapiens
1.8.3.1 0.62
-
cytochrome c mutant enzyme G473D, pH 7.5 Homo sapiens
1.8.3.1 0.78
-
cytochrome c mutant enzyme G473D, pH 7.0 Homo sapiens
1.8.3.1 1.35
-
sulfite mutant enzyme G473W, pH 9.0 Homo sapiens
1.8.3.1 1.67
-
cytochrome c mutant enzyme G473W, pH 6.0 Homo sapiens
1.8.3.1 1.8
-
sulfite mutant enzyme G473W, pH 7.0 Homo sapiens
1.8.3.1 1.95
-
cytochrome c mutant enzyme G473W, pH 8.5 Homo sapiens
1.8.3.1 2.48
-
sulfite mutant enzyme G473W, pH 8.5 Homo sapiens
1.8.3.1 2.94
-
cytochrome c mutant enzyme G473W, pH 7.0 Homo sapiens
1.8.3.1 3.83
-
cytochrome c mutant enzyme G473A, pH 6.0 Homo sapiens
1.8.3.1 4.15
-
sulfite mutant enzyme G473A, pH 6.0 Homo sapiens
1.8.3.1 12.4
-
cytochrome c wild type enzyme, pH 6.0 Homo sapiens
1.8.3.1 12.8
-
cytochrome c mutant enzyme G473A, pH 7.0 Homo sapiens
1.8.3.1 13.2
-
sulfite wild type enzyme, pH 6.0 Homo sapiens
1.8.3.1 15.1
-
sulfite mutant enzyme G473A, pH 10.0 Homo sapiens
1.8.3.1 15.9
-
sulfite mutant enzyme G473A, pH 7.0 Homo sapiens
1.8.3.1 17.7
-
sulfite wild type enzyme, pH 6.5 Homo sapiens
1.8.3.1 18.1
-
cytochrome c wild type enzyme, pH 7.0 Homo sapiens
1.8.3.1 21.6
-
sulfite mutant enzyme G473A, pH 7.4 Homo sapiens
1.8.3.1 24.2
-
sulfite wild type enzyme, pH 7.0 Homo sapiens
1.8.3.1 24.7
-
sulfite wild type enzyme, pH 7.5 Homo sapiens
1.8.3.1 25.4
-
cytochrome c mutant enzyme G473A, pH 8.5 Homo sapiens
1.8.3.1 25.7
-
sulfite wild type enzyme, pH 9.0 Homo sapiens
1.8.3.1 25.9
-
sulfite wild type enzyme, pH 8.0 Homo sapiens
1.8.3.1 26.2
-
sulfite mutant enzyme G473A, pH 8.0 Homo sapiens
1.8.3.1 26.3
-
sulfite wild type enzyme, pH 9.5 Homo sapiens
1.8.3.1 26.9
-
cytochrome c wild type enzyme, pH 8.5 Homo sapiens
1.8.3.1 26.9
-
sulfite wild type enzyme, pH 8.5 Homo sapiens
1.8.3.1 28.4
-
sulfite mutant enzyme G473A, pH 8.5 Homo sapiens
1.8.3.1 31.9
-
sulfite mutant enzyme G473A, pH 9.1 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.2.1 6 10
-
Homo sapiens
1.8.3.1 6 10
-
Homo sapiens