EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.2.1 | expressed in Escherichia coli strain TP1000 | Homo sapiens |
1.8.3.1 | expressed in Escherichia coli strain TP1000 | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.2.1 | G473A | mutant is able to dimerize and has steady-state activity comparable to that of the wild type, stopped-flow analysis of the reductive half-reaction of this variant yields a rate constant nearly 3 times higher than that of the wild type | Homo sapiens |
1.8.2.1 | G473D | monomer, mutant is severely impaired both in the ability to bind sulfite and in catalysis, with a second-order rate constant 5 orders of magnitude lower than that of the wild type, significant random-coil formation | Homo sapiens |
1.8.2.1 | G473W | monomer, mutant with 5fold higher activity than G473D and nearly wild-type activity at pH 7.0 when ferricyanide is the electron acceptor, significant random-coil formation | Homo sapiens |
1.8.2.1 | R212A/G473D | mutant is able to oligomerize but has undetectable activity, significant random-coil formation | Homo sapiens |
1.8.3.1 | G473A | mutant is able to dimerize and has steady-state activity comparable to that of the wild type, stopped-flow analysis of the reductive half-reaction of this variant yields a rate constant nearly 3 times higher than that of the wild type | Homo sapiens |
1.8.3.1 | G473D | monomer, mutant is severely impaired both in the ability to bind sulfite and in catalysis, with a second-order rate constant 5 orders of magnitude lower than that of the wild type, significant random-coil formation | Homo sapiens |
1.8.3.1 | G473W | monomer, mutant with 5fold higher activity than G473D and nearly wild-type activity at pH 7.0 when ferricyanide is the electron acceptor, significant random-coil formation | Homo sapiens |
1.8.3.1 | R212A/G473D | mutant is able to oligomerize but has undetectable activity, significant random-coil formation | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.2.1 | 0.00456 | - |
sulfite | wild type enzyme, pH 7.0, using ferricyanide as the electron acceptor | Homo sapiens | |
1.8.2.1 | 0.00605 | - |
sulfite | mutant enzyme G473A, pH 7.0, using ferricyanide as the electron acceptor | Homo sapiens | |
1.8.2.1 | 0.0149 | - |
sulfite | wild type enzyme, pH 8.6, using ferricyanide as the electron acceptor | Homo sapiens | |
1.8.2.1 | 0.191 | - |
sulfite | mutant enzyme G473A, pH 8.5, using ferricyanide as the electron acceptor | Homo sapiens | |
1.8.2.1 | 21.2 | - |
sulfite | mutant enzyme G473W, pH 7.0, using ferricyanide as the electron acceptor | Homo sapiens | |
1.8.2.1 | 24 | - |
sulfite | mutant enzyme G473W, pH 8.5, using ferricyanide as the electron acceptor | Homo sapiens | |
1.8.2.1 | 26.5 | - |
sulfite | mutant enzyme G473D, pH 7.0, using ferricyanide as the electron acceptor | Homo sapiens | |
1.8.2.1 | 41.4 | - |
sulfite | mutant enzyme G473D, pH 8.5, using ferricyanide as the electron acceptor | Homo sapiens | |
1.8.3.1 | 0.00043 | - |
cytochrome c | mutant enzyme G473A, pH 8.5 | Homo sapiens | |
1.8.3.1 | 0.00082 | - |
cytochrome c | mutant enzyme G473A, pH 7.0 | Homo sapiens | |
1.8.3.1 | 0.00123 | - |
cytochrome c | mutant enzyme G473A, pH 6.0 | Homo sapiens | |
1.8.3.1 | 0.00129 | - |
sulfite | wild type enzyme, pH 6.0 | Homo sapiens | |
1.8.3.1 | 0.00141 | - |
cytochrome c | wild type enzyme, pH 7.0 | Homo sapiens | |
1.8.3.1 | 0.00162 | - |
sulfite | wild type enzyme, pH 6.5 | Homo sapiens | |
1.8.3.1 | 0.00173 | - |
cytochrome c | wild type enzyme, pH 6.0 | Homo sapiens | |
1.8.3.1 | 0.00272 | - |
sulfite | wild type enzyme, pH 7.0 | Homo sapiens | |
1.8.3.1 | 0.00339 | - |
sulfite | wild type enzyme, pH 7.5 | Homo sapiens | |
1.8.3.1 | 0.00371 | - |
cytochrome c | mutant enzyme G473D, pH 8.6 | Homo sapiens | |
1.8.3.1 | 0.00435 | - |
sulfite | wild type enzyme, pH 8.0 | Homo sapiens | |
1.8.3.1 | 0.0044 | - |
cytochrome c | wild type enzyme, pH 8.5 | Homo sapiens | |
1.8.3.1 | 0.00453 | - |
sulfite | mutant enzyme G473A, pH 6.0 | Homo sapiens | |
1.8.3.1 | 0.00474 | - |
cytochrome c | mutant enzyme G473D, pH 8.0 | Homo sapiens | |
1.8.3.1 | 0.00536 | - |
sulfite | mutant enzyme G473A, pH 7.0 | Homo sapiens | |
1.8.3.1 | 0.00536 | - |
cytochrome c | mutant enzyme G473W, pH 8.5 | Homo sapiens | |
1.8.3.1 | 0.00825 | - |
sulfite | wild type enzyme, pH 8.5 | Homo sapiens | |
1.8.3.1 | 0.014 | - |
cytochrome c | mutant enzyme G473D, pH 7.5 | Homo sapiens | |
1.8.3.1 | 0.0172 | - |
sulfite | mutant enzyme G473A, pH 7.4 | Homo sapiens | |
1.8.3.1 | 0.0221 | - |
sulfite | wild type enzyme, pH 9.0 | Homo sapiens | |
1.8.3.1 | 0.0357 | - |
cytochrome c | mutant enzyme G473W, pH 7.0 | Homo sapiens | |
1.8.3.1 | 0.0362 | - |
cytochrome c | mutant enzyme G473D, pH 7.0 | Homo sapiens | |
1.8.3.1 | 0.0487 | - |
sulfite | mutant enzyme G473A, pH 8.0 | Homo sapiens | |
1.8.3.1 | 0.0635 | - |
cytochrome c | mutant enzyme G473D, pH 6.5 | Homo sapiens | |
1.8.3.1 | 0.0671 | - |
sulfite | wild type enzyme, pH 9.5 | Homo sapiens | |
1.8.3.1 | 0.0905 | - |
cytochrome c | mutant enzyme G473W, pH 6.0 | Homo sapiens | |
1.8.3.1 | 0.107 | - |
sulfite | mutant enzyme G473A, pH 8.5 | Homo sapiens | |
1.8.3.1 | 0.107 | - |
cytochrome c | mutant enzyme G473D, pH 6.0 | Homo sapiens | |
1.8.3.1 | 0.33 | - |
sulfite | mutant enzyme G473W, pH 7.0 | Homo sapiens | |
1.8.3.1 | 0.623 | - |
sulfite | mutant enzyme G473D, pH 7.0 | Homo sapiens | |
1.8.3.1 | 0.774 | - |
sulfite | mutant enzyme G473A, pH 9.1 | Homo sapiens | |
1.8.3.1 | 0.99 | - |
sulfite | mutant enzyme G473D, pH 6.5 | Homo sapiens | |
1.8.3.1 | 1.063 | - |
sulfite | mutant enzyme G473D, pH 7.5 | Homo sapiens | |
1.8.3.1 | 1.223 | - |
sulfite | mutant enzyme G473D, pH 8.0 | Homo sapiens | |
1.8.3.1 | 1.66 | - |
sulfite | mutant enzyme G473D, pH 6.0 | Homo sapiens | |
1.8.3.1 | 1.91 | - |
sulfite | mutant enzyme G473W, pH 6.0 | Homo sapiens | |
1.8.3.1 | 2.034 | - |
sulfite | mutant enzyme G473W, pH 8.5 | Homo sapiens | |
1.8.3.1 | 2.04 | - |
sulfite | mutant enzyme G473D, pH 8.5 | Homo sapiens | |
1.8.3.1 | 3.684 | - |
sulfite | mutant enzyme G473A, pH 10.0 | Homo sapiens | |
1.8.3.1 | 10.41 | - |
sulfite | mutant enzyme G473W, pH 9.0 | Homo sapiens | |
1.8.3.1 | 25.88 | - |
sulfite | mutant enzyme G473D, pH 9.1 | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.8.2.1 | Mo | consists of a single molybdenum atom coordinated through the dithiolene group of a single molybdopterin molecule | Homo sapiens | |
1.8.3.1 | Molybdenum | consists of a single molybdenum atom coordinated through the dithiolene group of a single molybdopterin molecule | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.2.1 | 52000 | - |
1 * 52000, mutant G473D, calculation from amino acid sequence | Homo sapiens |
1.8.2.1 | 52000 | - |
2 * 52000, wild type enzyme, sedimentation equilibrium analysis | Homo sapiens |
1.8.2.1 | 53500 | - |
1 * 53500, mutant G473D, gel filtration and sedimentation equilibrium analysis | Homo sapiens |
1.8.2.1 | 54500 | - |
1 * 54500, mutant G473W, sedimentation equilibrium analysis | Homo sapiens |
1.8.2.1 | 66600 | - |
1 * 66600, double mutant R212A/G473D, sedimentation equilibrium analysis | Homo sapiens |
1.8.2.1 | 101000 | - |
wild type enzyme, sedimentation equilibrium analysis | Homo sapiens |
1.8.3.1 | 52000 | - |
1 * 52000, mutant G473D, calculation from amino acid sequence | Homo sapiens |
1.8.3.1 | 52000 | - |
2 * 52000, wild type enzyme, sedimentation equilibrium analysis | Homo sapiens |
1.8.3.1 | 53500 | - |
1 * 53500, mutant G473D, gel filtration and sedimentation equilibrium analysis | Homo sapiens |
1.8.3.1 | 54500 | - |
1 * 54500, mutant G473W, sedimentation equilibrium analysis | Homo sapiens |
1.8.3.1 | 66600 | - |
1 * 66600, double mutant R212A/G473D, sedimentation equilibrium analysis | Homo sapiens |
1.8.3.1 | 101000 | - |
wild type enzyme, sedimentation equilibrium analysis | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.2.1 | Homo sapiens | - |
- |
- |
1.8.3.1 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.2.1 | phenyl-Sepharose column chromatography and Superdex 200 16/60 column gel filtration | Homo sapiens |
1.8.3.1 | phenyl-Sepharose column chromatography and Superdex 200 16/60 FPLC column gel filtration | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.2.1 | sulfite + ferricyanide + H2O | - |
Homo sapiens | sulfate + ferrocyanide | - |
? | |
1.8.3.1 | sulfite + cytochrome c | - |
Homo sapiens | sulfate + reduced cytochrome c | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.2.1 | dimer | 2 * 52000, wild type enzyme, sedimentation equilibrium analysis | Homo sapiens |
1.8.2.1 | monomer | 1 * 52000, mutant G473D, calculation from amino acid sequence | Homo sapiens |
1.8.2.1 | monomer | 1 * 53500, mutant G473D, gel filtration and sedimentation equilibrium analysis | Homo sapiens |
1.8.2.1 | monomer | 1 * 54500, mutant G473W, sedimentation equilibrium analysis | Homo sapiens |
1.8.2.1 | monomer | 1 * 66600, double mutant R212A/G473D, sedimentation equilibrium analysis | Homo sapiens |
1.8.3.1 | dimer | 2 * 52000, wild type enzyme, sedimentation equilibrium analysis | Homo sapiens |
1.8.3.1 | monomer | 1 * 52000, mutant G473D, calculation from amino acid sequence | Homo sapiens |
1.8.3.1 | monomer | 1 * 53500, mutant G473D, gel filtration and sedimentation equilibrium analysis | Homo sapiens |
1.8.3.1 | monomer | 1 * 54500, mutant G473W, sedimentation equilibrium analysis | Homo sapiens |
1.8.3.1 | monomer | 1 * 66600, double mutant R212A/G473D, sedimentation equilibrium analysis | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.2.1 | SO | - |
Homo sapiens |
1.8.2.1 | sulfite oxidase | - |
Homo sapiens |
1.8.3.1 | SO | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.2.1 | 25 | - |
- |
Homo sapiens |
1.8.3.1 | 25 | - |
- |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.3.1 | 0.14 | - |
sulfite | mutant enzyme G473D, pH 6.0 | Homo sapiens | |
1.8.3.1 | 0.28 | - |
sulfite | mutant enzyme G473D, pH 6.5 | Homo sapiens | |
1.8.3.1 | 0.34 | - |
cytochrome c | mutant enzyme G473D, pH 6.0 | Homo sapiens | |
1.8.3.1 | 0.42 | - |
sulfite | mutant enzyme G473D, pH 9.1 | Homo sapiens | |
1.8.3.1 | 0.48 | - |
cytochrome c | mutant enzyme G473D, pH 8.6 | Homo sapiens | |
1.8.3.1 | 0.5 | - |
sulfite | mutant enzyme G473D, pH 7.0 | Homo sapiens | |
1.8.3.1 | 0.53 | - |
cytochrome c | mutant enzyme G473D, pH 6.5 | Homo sapiens | |
1.8.3.1 | 0.54 | - |
sulfite | mutant enzyme G473D, pH 8.5 | Homo sapiens | |
1.8.3.1 | 0.57 | - |
sulfite | mutant enzyme G473D, pH 7.5 | Homo sapiens | |
1.8.3.1 | 0.58 | - |
sulfite | mutant enzyme G473D, pH 8.0 | Homo sapiens | |
1.8.3.1 | 0.59 | - |
cytochrome c | mutant enzyme G473D, pH 8.0 | Homo sapiens | |
1.8.3.1 | 0.6 | - |
sulfite | mutant enzyme G473W, pH 6.0 | Homo sapiens | |
1.8.3.1 | 0.62 | - |
cytochrome c | mutant enzyme G473D, pH 7.5 | Homo sapiens | |
1.8.3.1 | 0.78 | - |
cytochrome c | mutant enzyme G473D, pH 7.0 | Homo sapiens | |
1.8.3.1 | 1.35 | - |
sulfite | mutant enzyme G473W, pH 9.0 | Homo sapiens | |
1.8.3.1 | 1.67 | - |
cytochrome c | mutant enzyme G473W, pH 6.0 | Homo sapiens | |
1.8.3.1 | 1.8 | - |
sulfite | mutant enzyme G473W, pH 7.0 | Homo sapiens | |
1.8.3.1 | 1.95 | - |
cytochrome c | mutant enzyme G473W, pH 8.5 | Homo sapiens | |
1.8.3.1 | 2.48 | - |
sulfite | mutant enzyme G473W, pH 8.5 | Homo sapiens | |
1.8.3.1 | 2.94 | - |
cytochrome c | mutant enzyme G473W, pH 7.0 | Homo sapiens | |
1.8.3.1 | 3.83 | - |
cytochrome c | mutant enzyme G473A, pH 6.0 | Homo sapiens | |
1.8.3.1 | 4.15 | - |
sulfite | mutant enzyme G473A, pH 6.0 | Homo sapiens | |
1.8.3.1 | 12.4 | - |
cytochrome c | wild type enzyme, pH 6.0 | Homo sapiens | |
1.8.3.1 | 12.8 | - |
cytochrome c | mutant enzyme G473A, pH 7.0 | Homo sapiens | |
1.8.3.1 | 13.2 | - |
sulfite | wild type enzyme, pH 6.0 | Homo sapiens | |
1.8.3.1 | 15.1 | - |
sulfite | mutant enzyme G473A, pH 10.0 | Homo sapiens | |
1.8.3.1 | 15.9 | - |
sulfite | mutant enzyme G473A, pH 7.0 | Homo sapiens | |
1.8.3.1 | 17.7 | - |
sulfite | wild type enzyme, pH 6.5 | Homo sapiens | |
1.8.3.1 | 18.1 | - |
cytochrome c | wild type enzyme, pH 7.0 | Homo sapiens | |
1.8.3.1 | 21.6 | - |
sulfite | mutant enzyme G473A, pH 7.4 | Homo sapiens | |
1.8.3.1 | 24.2 | - |
sulfite | wild type enzyme, pH 7.0 | Homo sapiens | |
1.8.3.1 | 24.7 | - |
sulfite | wild type enzyme, pH 7.5 | Homo sapiens | |
1.8.3.1 | 25.4 | - |
cytochrome c | mutant enzyme G473A, pH 8.5 | Homo sapiens | |
1.8.3.1 | 25.7 | - |
sulfite | wild type enzyme, pH 9.0 | Homo sapiens | |
1.8.3.1 | 25.9 | - |
sulfite | wild type enzyme, pH 8.0 | Homo sapiens | |
1.8.3.1 | 26.2 | - |
sulfite | mutant enzyme G473A, pH 8.0 | Homo sapiens | |
1.8.3.1 | 26.3 | - |
sulfite | wild type enzyme, pH 9.5 | Homo sapiens | |
1.8.3.1 | 26.9 | - |
cytochrome c | wild type enzyme, pH 8.5 | Homo sapiens | |
1.8.3.1 | 26.9 | - |
sulfite | wild type enzyme, pH 8.5 | Homo sapiens | |
1.8.3.1 | 28.4 | - |
sulfite | mutant enzyme G473A, pH 8.5 | Homo sapiens | |
1.8.3.1 | 31.9 | - |
sulfite | mutant enzyme G473A, pH 9.1 | Homo sapiens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.8.2.1 | 6 | 10 | - |
Homo sapiens |
1.8.3.1 | 6 | 10 | - |
Homo sapiens |