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Literature summary extracted from

  • Sugimori, D.; Nakamura, M.
    Partial purification and some properties of a phospholipase C from Pseudomonas sp. strain KS3.2 (2006), Biosci. Biotechnol. Biochem., 70, 535-537.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.3 2-mercaptoethanol 2 mM, 40% inhibition Pseudomonas sp.
3.1.4.3 CoCl2 2 mM, 90% inhibition Pseudomonas sp.
3.1.4.3 CuSO3 2 mM, 96% inhibition Pseudomonas sp.
3.1.4.3 dithiothreitol 2 mM, 94% inhibition Pseudomonas sp.
3.1.4.3 EDTA 2 mM, 92% inhibition Pseudomonas sp.
3.1.4.3 FeCl2 2 mM, 94% inhibition Pseudomonas sp.
3.1.4.3 FeCl3 2 mM, 90% inhibition Pseudomonas sp.
3.1.4.3 HgCl2 2 mM, 92% inhibition Pseudomonas sp.
3.1.4.3 MnCl2 2 mM, 92% inhibition Pseudomonas sp.
3.1.4.3 phenylmethylsulfonyl fluoride 2 mM, 94% inhibition Pseudomonas sp.
3.1.4.3 SDS 2 mM, 90% inhibition Pseudomonas sp.
3.1.4.3 ZnCl2 2 mM, 89% inhibition Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.3 extracellular
-
 Pseudomonas sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.3 additional information enzyme belongs to the group of metallophospholipases C Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Pseudomonas sp.
-
-
-
3.1.4.3 Pseudomonas sp. KS3.2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3 partial Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 additional information phosphatidylethanolamine, sphingomyelin, phosphatidylinositol and phosphatidylserine are hydrolyzed at less than 1% of the activity with phosphatidylcholine Pseudomonas sp. ?
-
 ?
3.1.4.3 additional information phosphatidylethanolamine, sphingomyelin, phosphatidylinositol and phosphatidylserine are hydrolyzed at less than 1% of the activity with phosphatidylcholine Pseudomonas sp. KS3.2 ?
-
 ?
3.1.4.3 phosphatidic acid + H2O 9.04% of the activity with phosphatidylcholine Pseudomonas sp. ?
-
 ?
3.1.4.3 phosphatidic acid + H2O 9.04% of the activity with phosphatidylcholine Pseudomonas sp. KS3.2 ?
-
 ?
3.1.4.3 phosphatidylcholine + H2O
-
 Pseudomonas sp. 1,2-diacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylcholine + H2O
-
 Pseudomonas sp. KS3.2 1,2-diacylglycerol + phosphorylcholine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.3 50
-
-
 Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.4.3 35 60 35°C: about 50% of maximal activity, 60°C: about 50% of maximal activity Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.3 52
-
 pH 7.2, 30 min, 50% loss of activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.3 7.2
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.3 6.5 8.5 pH 6.5: about 50% of maximal activity, pH 8.5: about 50% of maximal activity Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.4.3 1 2 5°C, 3 h, about 55% loss of activity Pseudomonas sp.
3.1.4.3 6
-
 5°C, 3 h, about 70% loss of activity Pseudomonas sp.
3.1.4.3 7
-
 5°C, 3 h, about 35% loss of activity Pseudomonas sp.
3.1.4.3 8 9 5°C, 3 h, stable Pseudomonas sp.