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Literature summary extracted from

  • Banerjee, S.; Nandyala, A.; Podili, R.; Katoch, V.M.; Hasnain, S.E.
    Comparison of Mycobacterium tuberculosis isocitrate dehydrogenases (ICD-1 and ICD-2) reveals differences in coenzyme affinity, oligomeric state, pH tolerance and phylogenetic affiliation (2005), BMC Biochem., 6, 20.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.42 NaCl isoform ICD-2, increase in activity in up to 200 mM NaCl. Isoform ICD-1, no influence of NaCl. Above 200 mM, NaCl is inhibitory Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.42 NaCl isoform ICD-2, increase in activity in up to 200 mM NaCl. Isoform ICD-1, no influence of NaCl. Above 200 mM, NaCl is inhibitory Mycobacterium tuberculosis
1.1.1.42 NADPH isoform ICD-2, competitive inhibition Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.01
-
 DL-isocitrate isoform ICD-1, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.42 0.02
-
 DL-isocitrate isoform ICD-2, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.42 0.022
-
 DL-isocitrate isoform ICD-1, presence of Zn2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.42 0.125
-
 NADP+ isoform ICD-1, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.42 19.6
-
 NADP+ isoform ICD-2, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+ both isoforms ICD-1, ICD-2, saturation at 10 mM Mycobacterium tuberculosis
1.1.1.42 additional information no acitivty of isoforms with Mn2+ or Ca2+ Mycobacterium tuberculosis
1.1.1.42 Zn2+ isoform ICD-1, can replace Mg2+, saturation at 10 mM Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.42 49000
-
 2 * 49000, isoform ICD-1, 2 * 86000, isoform ICD-2, SDS-PAGE. Besides dimers, isoform ICD-1 forms some tetramer, ICD-2 forms trimer Mycobacterium tuberculosis
1.1.1.42 49000
-
 4 * 49000, isoform ICD-1, SDS-PAGE. Main form is dimer for isoform ICD-1 Mycobacterium tuberculosis
1.1.1.42 86000
-
 2 * 49000, isoform ICD-1, 2 * 86000, isoform ICD-2, SDS-PAGE. Besides dimers, isoform ICD-1 forms some tetramer, ICD-2 forms trimer Mycobacterium tuberculosis
1.1.1.42 86000
-
 and dimer, 3 * 86000, SDS-PAGE and chemical crosslinking, isoform ICD-2 Mycobacterium tuberculosis
1.1.1.42 100000
-
 gel filtration, main peak, isoform ICD-1 Mycobacterium tuberculosis
1.1.1.42 180000
-
 gel filtration, isoform ICD-2 Mycobacterium tuberculosis
1.1.1.42 200000
-
 gel filtration, isoform ICD-1 Mycobacterium tuberculosis
1.1.1.42 320000
-
 gel filtration, isoform ICD-2 Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Mycobacterium tuberculosis
-
 isoforms ICD-1 and ICD-2
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.1.1.42 isoform ICD-2, renaturation of protein inactivated for 30 min at 65°C by slow cooling till 55°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 DL-isocitrate + NADP+
-
 Mycobacterium tuberculosis 2-oxoglutarate + CO2 + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.42 dimer 2 * 49000, isoform ICD-1, 2 * 86000, isoform ICD-2, SDS-PAGE. Besides dimers, isoform ICD-1 forms some tetramer, ICD-2 forms trimer Mycobacterium tuberculosis
1.1.1.42 tetramer 4 * 49000, isoform ICD-1, SDS-PAGE. Main form is dimer for isoform ICD-1 Mycobacterium tuberculosis
1.1.1.42 trimer and dimer, 3 * 86000, SDS-PAGE and chemical crosslinking, isoform ICD-2 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.42 20 40 isoform ICD-2 Mycobacterium tuberculosis
1.1.1.42 40
-
 isoform ICD-1 Mycobacterium tuberculosis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.42 60
-
 isoform ICD-1, 40% residual activity, ICD-2, 5% residual activity Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.42 65
-
 30 min, isoform ICD-1, irreversible thermal inactivation, isoform ICD-2, renaturation of inactivated protein by slow cooling till 55°C Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.42 1.9
-
 DL-isocitrate isoform ICD-1, presence of Zn2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.42 3.8
-
 DL-isocitrate isoform ICD-1, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.42 4
-
 NADP+ isoform ICD-1, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.42 19.6
-
 DL-isocitrate isoform ICD-2, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.42 37.4
-
 NADP+ isoform ICD-2, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 7.5
-
 both isoforms ICD-1, ICD-2 Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.42 5.5
-
 isoform ICD-1, 30% of maximum activity, isoform ICD-2, 10% of maximum activity Mycobacterium tuberculosis
1.1.1.42 9.5
-
 isoform ICD-1, 90% of maximum activity, isoform ICD-2, less than 40% of maximum activity Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 additional information NAD+ may not replace NADP+ Mycobacterium tuberculosis
1.1.1.42 NADP+ required by both isoforms ICD-1 and ICD-2 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.42 0.0046
-
 NADPH isoform ICD-2, pH 7.5, 25°C Mycobacterium tuberculosis