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Literature summary extracted from

  • Clamagirand, C.; Joudiou, C.; Cohen, P.
    Dactylysin (2004), Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 1042-1044.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.60 (Leu5)-enkephalin inhibitor of dactylysin towards substance P Xenopus laevis
3.4.24.60 (Met5)-enkephalin inhibitor of dactylysin towards substance P Xenopus laevis
3.4.24.60 1,10-phenanthroline strongly inhibits Xenopus laevis
3.4.24.60 DTT
-
Xenopus laevis
3.4.24.60 EDTA strongly inhibits Xenopus laevis
3.4.24.60 EGTA strongly inhibits Xenopus laevis
3.4.24.60 additional information not inhibited by TIMPs and 1mM captopril Xenopus laevis
3.4.24.60 phosphoramidon
-
Xenopus laevis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.60 0.011
-
(Arg0,Leu6)-enkephalin
-
Xenopus laevis
3.4.24.60 0.018
-
Somatostatin-14
-
Xenopus laevis
3.4.24.60 0.02
-
Substance P
-
Xenopus laevis
3.4.24.60 0.028
-
antibacterial peptide PGLa
-
Xenopus laevis
3.4.24.60 0.041
-
bradykinin
-
Xenopus laevis
3.4.24.60 0.05
-
dynorphin A
-
Xenopus laevis
3.4.24.60 0.05
-
atrial natriuretic factor
-
Xenopus laevis
3.4.24.60 0.063
-
angiotensin II
-
Xenopus laevis
3.4.24.60 0.079
-
(Leu5,Arg6)-enkephalin
-
Xenopus laevis
3.4.24.60 0.125
-
Neurokinin A
-
Xenopus laevis
3.4.24.60 0.125
-
beta-amyloid derived peptide
-
Xenopus laevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.60 14000
-
subunit that retains the proteolytic activity Xenopus laevis
3.4.24.60 90000 100000
-
Xenopus laevis

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.60 Xenopus laevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.60 2000fold purified by a four-step fractionation including successively ion-exchange column, hydrophobic chromatographies and gel filtration Xenopus laevis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.60 gland
-
Xenopus laevis
-
3.4.24.60 skin
-
Xenopus laevis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.60 (Arg0,Leu6)-enkephalin + H2O cleavage of Phe4-Leu5 Xenopus laevis ?
-
?
3.4.24.60 (Leu5,Arg6)-enkephalin + H2O cleavage of Phe4-Leu5 Xenopus laevis YGGF + Leu-Arg
-
?
3.4.24.60 angiotensin II + H2O cleavage of Tyr4-Ile5 Xenopus laevis ?
-
?
3.4.24.60 Antibacterial peptide PGLa + H2O
-
Xenopus laevis ?
-
?
3.4.24.60 atrial natriuretic factor + H2O cleavage of Ser-Phe Xenopus laevis LGCNS + Phe-Arg-Tyr
-
?
3.4.24.60 beta-amyloid derived peptide + H2O cleavage of Gly-Leu Xenopus laevis ?
-
?
3.4.24.60 bradykinin + H2O cleavage of Gly4-Phe5 Xenopus laevis ?
-
?
3.4.24.60 dynorphin A + H2O
-
Xenopus laevis ?
-
?
3.4.24.60 dynorphin A1-16 + H2O cleavage of Phe-Leu Xenopus laevis ?
-
?
3.4.24.60 kermit + H2O cleavage of the peptide link Ser-Phe Xenopus laevis ?
-
?
3.4.24.60 additional information no cleavage of (Leu5)-enkephalin and (Met5)-enkephalin Xenopus laevis ?
-
?
3.4.24.60 Neurokinin A + H2O cleavage of Gly-Leu Xenopus laevis ?
-
?
3.4.24.60 neurokinin A + H2O
-
Xenopus laevis HKTDSFVG + Leu-Met-NH2
-
?
3.4.24.60 peptide PglA + H2O antimicrobial peptide of Xenopus laevis skin secretion predominantly cleaved at the Lys12-Ile13 bond Xenopus laevis ?
-
?
3.4.24.60 Somatostatin-14 + H2O cleavage of Phe-Phe in the intersulfide bridge motif Xenopus laevis ?
-
?
3.4.24.60 substance P + H2O cleavage of Gly9-Leu10 Xenopus laevis RPKQQF + Phe-Gly + Leu-Met-NH2
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.60 peptide hormone inactivating enzyme
-
Xenopus laevis
3.4.24.60 PHIE
-
Xenopus laevis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.60 0.0001
-
acid-pretreated corn stover 41% inhibition Xenopus laevis
3.4.24.60 0.0001
-
1,10-phenanthroline 98% inhibition Xenopus laevis
3.4.24.60 0.001
-
DTT 70% inhibition when tested on kermit Xenopus laevis