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Literature summary extracted from

  • Szabo, Z.; Albers, S.V.; Driessen, A.J.
    Active-site residues in the type IV prepilin peptidase homologue PibD from the archaeon Sulfolobus solfataricus (2006), J. Bacteriol., 188, 1437-1443.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.23.43 expression in Escherichia coli in inner membrane vesicles Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.23.43 D157A site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Saccharolobus solfataricus
3.4.23.43 D173A site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Saccharolobus solfataricus
3.4.23.43 D180A site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Saccharolobus solfataricus
3.4.23.43 D187A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Saccharolobus solfataricus
3.4.23.43 D187A/D188A site-directed mutagenesis, inactive mutant Saccharolobus solfataricus
3.4.23.43 D188A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Saccharolobus solfataricus
3.4.23.43 D207N site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Saccharolobus solfataricus
3.4.23.43 D23A site-directed mutagenesis, inactive mutant Saccharolobus solfataricus
3.4.23.43 D30A site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Saccharolobus solfataricus
3.4.23.43 D80A site-directed mutagenesis, inactive mutant Saccharolobus solfataricus
3.4.23.43 D80E site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Saccharolobus solfataricus
3.4.23.52 D157A the activity level of the mutant is comparable to that of the wild type enzyme Saccharolobus solfataricus
3.4.23.52 D173A the activity level of the mutant is comparable to that of the wild type enzyme Saccharolobus solfataricus
3.4.23.52 D180A the activity level of the mutant is comparable to that of the wild type enzyme Saccharolobus solfataricus
3.4.23.52 D187A the mutant shows an apparent reduction in activity Saccharolobus solfataricus
3.4.23.52 D187A/D188A the mutant does not cleave preflagellin Saccharolobus solfataricus
3.4.23.52 D188A the mutant shows about wild type activity Saccharolobus solfataricus
3.4.23.52 D207N the activity level of the mutant is comparable to that of the wild type enzyme Saccharolobus solfataricus
3.4.23.52 D23A the mutant does not cleave preflagellin Saccharolobus solfataricus
3.4.23.52 D30A the activity level of the mutant is comparable to that of the wild type enzyme Saccharolobus solfataricus
3.4.23.52 D80A the mutant does not cleave preflagellin Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.52 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride
-
 Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.23.43 membrane membrane topology, overview Saccharolobus solfataricus 16020
-
3.4.23.52 membrane
-
 Saccharolobus solfataricus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.23.43 preflagellin + H2O Saccharolobus solfataricus
-
 flagellin + preflagellin leader peptide
-
 ?
3.4.23.43 prepilin type IV + H2O Saccharolobus solfataricus
-
 pilin type IV + prepilin type IV leader peptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.43 Saccharolobus solfataricus Q6IFS8 gene pibD
-
3.4.23.52 Saccharolobus solfataricus Q6IFS8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.43 recombinant enzyme from Escherichia coli inner membrane vesicles Saccharolobus solfataricus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.23.43 typically cleaves a -Gly-/-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine Asp80 and Asp23 are the catalytic residues Saccharolobus solfataricus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.43 additional information the enzyme cleaves sugar-binding proteins unique to Sulfolobus solfataricus Saccharolobus solfataricus ?
-
 ?
3.4.23.43 preflagellin + H2O
-
 Saccharolobus solfataricus flagellin + preflagellin leader peptide
-
 ?
3.4.23.43 prepilin type IV + H2O
-
 Saccharolobus solfataricus pilin type IV + prepilin type IV leader peptide
-
 ?
3.4.23.52 pre-glucose-binding protein + H2O
-
 Saccharolobus solfataricus glucose-binding protein + ?
-
 ?
3.4.23.52 preflagellin + H2O most efficient substrate Saccharolobus solfataricus flagellin + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.23.43 More the enzyme belongs to the family of aspartic proteases Saccharolobus solfataricus
3.4.23.43 PibD
-
 Saccharolobus solfataricus
3.4.23.43 preflagellin peptidase
-
 Saccharolobus solfataricus
3.4.23.43 type IV prepilin peptidase
-
 Saccharolobus solfataricus
3.4.23.52 PibD
-
 Saccharolobus solfataricus