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Literature summary extracted from

  • Sonawane, A.M.; Roehm, K.H.
    A functional gltB gene is essential for utilization of acidic amino acids and expression of periplasmic glutaminase/asparaginase (PGA) by Pseudomonas putida KT2440 (2004), Mol. Genet. Genomics, 271, 33-39.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.38
-
 Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.38 additional information transposon mutagenesis with self-cloning promoter probe vector Tn5-OT182 for inactivation by disruption of gene gltB, gltB mutants lose the ability to utilize amino acids as carbon source, inactivation of gene gltB and other glutamate-induced genes by random mutagenesis, phenotype, overview Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.38 periplasm
-
 Pseudomonas putida
-
-
3.5.1.38 periplasm a functional gltB gene (encoding a subunit of EC 1.4.1.13) is essential for utilization of acidic amino acids and expression of periplasmic glutaminase/asparaginase Pseudomonas putida
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.38 L-asparagine + H2O Pseudomonas putida
-
 L-aspartate + NH3
-
 ?
3.5.1.38 L-asparagine + H2O Pseudomonas putida KT 2240
-
 L-aspartate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O Pseudomonas putida
-
 L-glutamate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O Pseudomonas putida KT 2240
-
 L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.38 Pseudomonas putida
-
-
-
3.5.1.38 Pseudomonas putida
-
 gene gltB
-
3.5.1.38 Pseudomonas putida KT 2240
-
-
-
3.5.1.38 Pseudomonas putida KT 2240
-
 gene gltB
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.38 additional information the organism is able to grow on asparagine and glutamine as sole carbon and nitrogen source Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.38 additional information
-
 intracellular amino acid levels of wild-type and mutant strains Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.38 L-asparagine + H2O
-
 Pseudomonas putida L-aspartate + NH3
-
 ?
3.5.1.38 L-asparagine + H2O
-
 Pseudomonas putida KT 2240 L-aspartate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O
-
 Pseudomonas putida L-glutamate + NH3
-
 ?
3.5.1.38 L-glutamine + H2O
-
 Pseudomonas putida KT 2240 L-glutamate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.38 glutaminase/asparaginase
-
 Pseudomonas putida
3.5.1.38 periplasmic glutaminase/asparaginase
-
 Pseudomonas putida
3.5.1.38 PGA
-
 Pseudomonas putida