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localization of tabersonine 16-hydroxylase and 16-OH tabersonine-16-O-methyltransferase to leaf epidermal cells defines them as a major site of precursor biosynthesis in the vindoline pathway in Catharanthus roseus

Murata, J.; De Luca, V.; Plant J. 44, 581-594 (2005)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.14.19.62
additional information
involved in vindoline biosynthesis
Catharanthus roseus
Cloned(Commentary)
EC Number
Commentary
Organism
1.14.14.103
T16H expression analysis in leaves, overview, recombinant expression in Escherichia coli
Catharanthus roseus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.103
additional information
Catharanthus roseus
the enzyme is involved in biosynthesis of precursors in the vindoline pathway in Catharanthus roseus leaf epidermal cells, biosynthesis of anticancer dimeric alkaloids vinblastine and vincristine, overview
?
-
-
-
1.14.14.103
tabersonine + NADPH + H+ + O2
Catharanthus roseus
-
16-hydroxytabersonine + NADP+ + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.103
Catharanthus roseus
-
-
-
1.14.19.62
Catharanthus roseus
-
-
-
3.2.1.105
Catharanthus roseus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.103
epidermal cell
predominantly
Catharanthus roseus
-
1.14.14.103
leaf
quantitative expression analysis in leaves
Catharanthus roseus
-
1.14.14.103
additional information
localization study
Catharanthus roseus
-
1.14.19.62
epidermal cell
leaf epidermal cells are biosynthetically competent to produce secologanin precursors that are converted via many enzymatic transformations to make 16-methoxytabersonine, which is then transported to cells within Catharanthus leaves to complete the last three or four enzymatic transformations to make vindoline
Catharanthus roseus
-
1.14.19.62
leaf
-
Catharanthus roseus
-
3.2.1.105
leaf epidermis
abaxial and adaxial
Catharanthus roseus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.103
additional information
the enzyme is involved in biosynthesis of precursors in the vindoline pathway in Catharanthus roseus leaf epidermal cells, biosynthesis of anticancer dimeric alkaloids vinblastine and vincristine, overview
670558
Catharanthus roseus
?
-
-
-
-
1.14.14.103
tabersonine + NADPH + H+ + O2
-
670558
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
3.2.1.105
strictosidine + H2O
-
670558
Catharanthus roseus
cathenamine
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.14.14.103
37
-
assay at
Catharanthus roseus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.103
cytochrome P450
-
Catharanthus roseus
Application (protein specific)
EC Number
Application
Commentary
Organism
1.14.19.62
additional information
involved in vindoline biosynthesis
Catharanthus roseus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.103
T16H expression analysis in leaves, overview, recombinant expression in Escherichia coli
Catharanthus roseus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.103
cytochrome P450
-
Catharanthus roseus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.103
additional information
Catharanthus roseus
the enzyme is involved in biosynthesis of precursors in the vindoline pathway in Catharanthus roseus leaf epidermal cells, biosynthesis of anticancer dimeric alkaloids vinblastine and vincristine, overview
?
-
-
-
1.14.14.103
tabersonine + NADPH + H+ + O2
Catharanthus roseus
-
16-hydroxytabersonine + NADP+ + H2O
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.103
epidermal cell
predominantly
Catharanthus roseus
-
1.14.14.103
leaf
quantitative expression analysis in leaves
Catharanthus roseus
-
1.14.14.103
additional information
localization study
Catharanthus roseus
-
1.14.19.62
epidermal cell
leaf epidermal cells are biosynthetically competent to produce secologanin precursors that are converted via many enzymatic transformations to make 16-methoxytabersonine, which is then transported to cells within Catharanthus leaves to complete the last three or four enzymatic transformations to make vindoline
Catharanthus roseus
-
1.14.19.62
leaf
-
Catharanthus roseus
-
3.2.1.105
leaf epidermis
abaxial and adaxial
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.103
additional information
the enzyme is involved in biosynthesis of precursors in the vindoline pathway in Catharanthus roseus leaf epidermal cells, biosynthesis of anticancer dimeric alkaloids vinblastine and vincristine, overview
670558
Catharanthus roseus
?
-
-
-
-
1.14.14.103
tabersonine + NADPH + H+ + O2
-
670558
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
3.2.1.105
strictosidine + H2O
-
670558
Catharanthus roseus
cathenamine
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.14.14.103
37
-
assay at
Catharanthus roseus