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Literature summary extracted from

  • Samanani, N.; Alcantara, J.; Bourgault, R.; Zulak, K.G.; Facchini, P.J.
    The role of phloem sieve elements and laticifers in the biosynthesis and accumulation of alkaloids in opium poppy (2006), Plant J., 47, 547-563.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.116 additional information localization of seven biosynthetic enzymes to the sieve elements, unique, cell type-specific biosynthesis of benzylisoquinoline alkaloids in the opium poppy Papaver somniferum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.102 heterologous expression Papaver somniferum
2.1.1.116 into pRSETB and introduced into Escherichia coli strain ER2566 pLys S Papaver somniferum
2.1.1.128 expressed as His-tag fusion protein in Escherichia coli ER2566pLys S Papaver somniferum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.247 cytosol
-
 Papaver somniferum 5829
-
1.14.14.102 endoplasmic reticulum membrane membrane-bound, in sieve element ER Papaver somniferum 5789
-
1.14.14.102 additional information subcellular localization study Papaver somniferum
-
-
2.1.1.116 cytoplasm
-
 Papaver somniferum 5737
-
2.1.1.128 cytoplasm immunogold labelling studies show the strict association with electron dense regions of the peripheral cytoplasm of sieve elements Papaver somniferum 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.102 Fe2+ P450-dependent hydroxylase Papaver somniferum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.116 39400
-
 immunoblot analysis Papaver somniferum
2.1.1.128 38500
-
 x * 38500, immunoblotting Papaver somniferum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.102 (S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2 Papaver somniferum the catalyzes step 5 in the unique, cell type-specific biosynthesis of benzylisoquinoline alkaloids in the opium poppy, biosynthetic pathway leading from L-DOPA to morphine, overview (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2.1.1.128 S-adenosyl-L-methionine + (S)-norcoclaurine Papaver somniferum putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis S-adenosyl-L-homocysteine + (S)-coclaurine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.247 Papaver somniferum
-
 opium poppy
-
1.14.14.102 Papaver somniferum
-
 cv. Louisiana
-
1.21.3.3 Papaver somniferum
-
-
-
2.1.1.116 Papaver somniferum
-
 cultivars Louisiana and Marianne
-
2.1.1.128 Papaver somniferum
-
 cv. Louisiana and Marianne
-
2.1.1.140 Papaver somniferum
-
 L. cv. Marianne and cv. Louisianne
-
2.3.1.150 Papaver somniferum Q94FT4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.247 Ni2+-charged HiTrap column chromatography Papaver somniferum
1.14.14.102 recombinant enzyme Papaver somniferum
2.1.1.116 by Ni2+ affinity chromatography Papaver somniferum
2.1.1.128 recombinant protein using His-tag Papaver somniferum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.247 hypocotyl
-
 Papaver somniferum
-
1.1.1.247 root
-
 Papaver somniferum
-
1.14.14.102 phloem localization in the sieve elements adjacent or proximal to laticifers in the root and hypocotyl of opium poppy seedlings Papaver somniferum
-
1.14.14.102 root phloem Papaver somniferum
-
1.14.14.102 seedling root phloem, the abundance of the enzyme increases rapidly between 1 and 3 days after seed germination Papaver somniferum
-
1.21.3.3 hypocotyl of seedling, localization in the sieve elelments Papaver somniferum
-
1.21.3.3 root of seedling, localization in the sieve elements Papaver somniferum
-
1.21.3.3 seedling sieve elements of root and hypocotyl, abundance of enzyme increases rapidly between 1 and 3 days of germination Papaver somniferum
-
2.1.1.116 flower bud higher levels of 4'OMT mRNA in cultivar Marianne, compared with cultivar Louisiana Papaver somniferum
-
2.1.1.116 hypocotyl
-
 Papaver somniferum
-
2.1.1.116 leaf lowest gene transcripts Papaver somniferum
-
2.1.1.116 phloem restricted to sieve elements adjacent or proximal to laticifers Papaver somniferum
-
2.1.1.116 root highest gene transcripts Papaver somniferum
-
2.1.1.116 seedling abundance of the enzyme increases rapidly between 1 and 3 days after seed germination Papaver somniferum
-
2.1.1.116 stem highest gene transcripts and levels of protein Papaver somniferum
-
2.1.1.128 flower low enzyme level Papaver somniferum
-
2.1.1.128 leaf low enzyme level Papaver somniferum
-
2.1.1.128 additional information gene transcripts detected in all organs with highest levels in root and stem and lowest in leaf Papaver somniferum
-
2.1.1.128 phloem sieve elements of the phloem adjacent or proximal to laticifers Papaver somniferum
-
2.1.1.128 root
-
 Papaver somniferum
-
2.1.1.128 seed constant enzyme level over 16 days of germination Papaver somniferum
-
2.1.1.128 stem
-
 Papaver somniferum
-
2.1.1.140 phloem sieve elements of the phloem adjacent or proximal to laticifers. Sieve elements in the root and hypocotyl of opium poppy seedlings Papaver somniferum
-
2.3.1.150 phloem enzyme protein is restricted to sieve elements of the phloem adjacent or proximal to laticifers. Corresponding gene transcripts are found in the companion cell paired with each sieve element Papaver somniferum
-
2.3.1.150 seed abundance of enzyme increases rapidly between 1 and 3 days of seed germination Papaver somniferum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.247 Codeinone + NADPH
-
 Papaver somniferum Codeine + NADP+
-
 ?
1.1.1.247 Morphinone + NADPH
-
 Papaver somniferum Morphine + NADP+
-
 ?
1.14.14.102 (S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
 Papaver somniferum (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.102 (S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2 the catalyzes step 5 in the unique, cell type-specific biosynthesis of benzylisoquinoline alkaloids in the opium poppy, biosynthetic pathway leading from L-DOPA to morphine, overview Papaver somniferum (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2.1.1.128 S-adenosyl-L-methionine + (S)-norcoclaurine putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis Papaver somniferum S-adenosyl-L-homocysteine + (S)-coclaurine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.128 ? x * 38500, immunoblotting Papaver somniferum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.247 codeinone reductase
-
 Papaver somniferum
1.1.1.247 COR
-
 Papaver somniferum
1.14.14.102 (S)-N-methylcoclaurine 3'-hydroxylase
-
 Papaver somniferum
1.14.14.102 CYP80B1
-
 Papaver somniferum
2.1.1.116 (S)-3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase
-
 Papaver somniferum
2.1.1.116 4'OMT
-
 Papaver somniferum
2.1.1.128 (S)-norcoclaurine 6-O-methyltransferase
-
 Papaver somniferum
2.1.1.128 6OMT
-
 Papaver somniferum
2.1.1.140 (S)-Coclaurine N-methyltransferase
-
 Papaver somniferum
2.1.1.140 CNMT
-
 Papaver somniferum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.247 NADPH
-
 Papaver somniferum
1.14.14.102 cytochrome P450 P450-dependent hydroxylase Papaver somniferum