EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.8 | expressed in Escherichia coli | Mycobacterium tuberculosis |
1.8.4.2 | expressed in Escherichia coli strain D5alpha | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.1.8 | 14000 | - |
2 * 14000, SDS-PAGE | Mycobacterium tuberculosis |
1.8.1.8 | 29000 | - |
gel filtration | Mycobacterium tuberculosis |
1.8.4.2 | 14000 | - |
2 * 14000, SDS-PAGE | Mycobacterium tuberculosis |
1.8.4.2 | 14056 | - |
2 * 14056, unmodified recombinant WhiB1, MALDI-TOF mass spectrometry | Mycobacterium tuberculosis |
1.8.4.2 | 14284 | - |
2 * 14284, reduced protein after alkylation corresponding to the theoretical mass of the recombinant protein with four iodoacetamide mediated modifications of cysteines, MALDI-TOF mass spectrometry | Mycobacterium tuberculosis |
1.8.4.2 | 29000 | - |
gel filtration | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.8 | Mycobacterium tuberculosis | - |
- |
- |
1.8.1.8 | Mycobacterium tuberculosis Rv3219 | - |
- |
- |
1.8.4.2 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.8 | Ni2+-NTA affinity chromatography | Mycobacterium tuberculosis |
1.8.4.2 | Ni2+-NTA affinity chromatography | Mycobacterium tuberculosis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.8.1.8 | additional information | - |
cell extract: 1.56 U/mg, after purification: 8.17 U/mg, one unit is defined as the amount of protein that produces an increase in 0.1 OD in 1 min at 650 nm | Mycobacterium tuberculosis |
1.8.4.2 | additional information | - |
1.56 U/mg in the cell extract, 8.17 U/mg after Ni2+-NTA chromatography purification, one unit is defined as the amount of protein that produces an increase in 0.1 OD in 1 min at 650 nm | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.8 | insulin disulfide + ? | - |
Mycobacterium tuberculosis | ? | - |
? | |
1.8.1.8 | insulin disulfide + ? | - |
Mycobacterium tuberculosis Rv3219 | ? | - |
? | |
1.8.4.2 | GSH + protein disulfide | - |
Mycobacterium tuberculosis | GSSG + protein-dithiol | - |
? | |
1.8.4.2 | GSH + protein disulfide | - |
Mycobacterium tuberculosis Rv3219 | GSSG + protein-dithiol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.1.8 | homodimer | 2 * 14000, SDS-PAGE | Mycobacterium tuberculosis |
1.8.4.2 | homodimer | 2 * 14000, SDS-PAGE | Mycobacterium tuberculosis |
1.8.4.2 | homodimer | 2 * 14056, unmodified recombinant WhiB1, MALDI-TOF mass spectrometry | Mycobacterium tuberculosis |
1.8.4.2 | homodimer | 2 * 14284, reduced protein after alkylation corresponding to the theoretical mass of the recombinant protein with four iodoacetamide mediated modifications of cysteines, MALDI-TOF mass spectrometry | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.8 | protein disulfide reductase | - |
Mycobacterium tuberculosis |
1.8.1.8 | WhiB1 | wild type enzyme | Mycobacterium tuberculosis |
1.8.1.8 | WhiB1/Rv3219 | recombinant enzyme | Mycobacterium tuberculosis |
1.8.4.2 | WhiB1 | - |
Mycobacterium tuberculosis |
1.8.4.2 | WhiB1/Rv3219 | - |
Mycobacterium tuberculosis |