EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.9 | - |
Gluconobacter oxydans |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.9 | hanging drop vapor diffusion method, crystal structure of the holoenzyme to 1.9 A resolution | Gluconobacter oxydans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.9 | D38S/M39R | the mutant enzyme is able to exclusively use NADP+, with no loss of activity | Gluconobacter oxydans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.9 | 0.0205 | - |
NADP+ | pH 7.0, mutant enzyme D38S/M39R | Gluconobacter oxydans | |
1.1.1.9 | 0.348 | - |
NAD+ | pH 7.0, wild-type enzyme | Gluconobacter oxydans | |
1.1.1.9 | 13.7 | - |
xylitol | pH 7.0, cofactor: NAD+, wild-type enzyme | Gluconobacter oxydans | |
1.1.1.9 | 100 | - |
xylitol | pH 7.0, cofactor: NADP+ mutant enzyme D38S/M39R | Gluconobacter oxydans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.9 | Gluconobacter oxydans | Q8GR61 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.9 | - |
Gluconobacter oxydans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.9 | xylitol + NAD+ | - |
Gluconobacter oxydans | D-xylulose + NADH + H+ | - |
? | |
1.1.1.9 | xylitol + NADP+ | wild-type enzyme shows no activity with NADP+, mutant enzyme D38S/M39R is able to exclusively use NADP+, with no loss of activity | Gluconobacter oxydans | D-xylulose + NADPH + H+ | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.9 | 0.206 | - |
NADP+ | pH 7.0, mutant enzyme D38S/M39R | Gluconobacter oxydans | |
1.1.1.9 | 17.9 | - |
xylitol | pH 7.0, cofactor: NADP+mutant enzyme D38S/M39R | Gluconobacter oxydans | |
1.1.1.9 | 24.6 | - |
xylitol | pH 7.0, cofactor: NAD+, wild-type enzyme | Gluconobacter oxydans | |
1.1.1.9 | 27.2 | - |
NAD+ | pH 7.0, wild-type enzyme | Gluconobacter oxydans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.9 | NAD+ | NAD+ specificity is largely conferred by Asp38, which interacts with the hydroxyls of the adenosine ribose | Gluconobacter oxydans | |
1.1.1.9 | NADP+ | wild-type enzyme shows no activity with NADP+, mutant enzyme D38S/M39R is able to exclusively use NADP+, with no loss of activity | Gluconobacter oxydans |