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Literature summary extracted from

  • Rukamp, B.J.; Kam, C.M.; Natarajan, S.; Bolton, B.W.; Smyth, M.J.; Kelly, J.M.; Powers, J.C.
    Subsite specificities of granzyme M: a study of inhibitors and newly synthesized thiobenzyl ester substrates (2004), Arch. Biochem. Biophys., 422, 9-22.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B2 into transfer vector pBacPAK8, expressed in Sf21 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B2 additional information a series of peptide phosphonates and chloromethyl ketones tested, neither inhibitor type proved to be especially potent toward the enzyme, including the general serine protease inhibitor, 3,4-dichloroisocoumarin Homo sapiens
3.4.21.B2 additional information a series of peptide phosphonates and chloromethyl ketones tested, neither inhibitor type proved to be especially potent toward the enzyme, including the general serine protease inhibitor, 3,4-dichloroisocoumarin Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.B2 0.0263
-
 N2-[(benzyloxy)carbonyl]-N6-(tert-butoxycarbonyl)-L-lysyl-L-prolyl-L-methionyl thiobenzyl ester
-
 Homo sapiens
3.4.21.B2 0.0334
-
 N2-Cbz-L-Asp(O-tBut)-L-Pro-L-Met thiobenzyl ester
-
 Homo sapiens
3.4.21.B2 0.036
-
 N-benzyloxycarbonyl-phenylalanyl-prolyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 0.0366
-
 N2-CBZ-O3-tBut-L-Ser-L-Pro-L-Met-SBzl murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.0423
-
 N2-CBZ-O3-tBut-L-Ser-L-Pro-L-Met-SBzl
-
 Homo sapiens
3.4.21.B2 0.079
-
 N-benzyloxycarbonyl-alanyl-alanyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.083
-
 N2-[(benzyloxy)carbonyl]-N6-(tert-butoxycarbonyl)-L-lysyl-L-prolyl-L-methionyl thiobenzyl ester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.0915
-
 N-benzyloxycarbonyl-prolyl-prolyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 0.129
-
 N2-Cbz-L-Asp(O-tBut)-L-Pro-L-Met thiobenzyl ester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.181
-
 N-benzyloxycarbonyl-phenylalanyl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.184
-
 N-benzyloxycarbonyl-alanyl-alanyl-methionine-thiobenzylester murine granzyme M without bovine serum albumin Homo sapiens
3.4.21.B2 0.188
-
 N-benzyloxycarbonyl-alanyl-alanyl-prolyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 0.21
-
 N-benzyloxycarbonyl-alanyl-seryl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.217
-
 N-benzyloxycarbonyl-prolyl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.22
-
 N-benzyloxycarbonyl-alanyl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.236
-
 N-benzyloxycarbonyl-alanyl-alanyl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.267
-
 N-benzyloxycarbonyl-alanyl-aspartyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.275
-
 N-benzyloxycarbonyl-alanyl-prolyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 0.361
-
 N-benzyloxycarbonyl-lysyl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.418
-
 N-benzyloxycarbonyl-aspartyl-prolyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 0.441
-
 N-benzyloxycarbonyl-aspartyl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.635
-
 N-benzyloxycarbonyl-alanyl-alanyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 0.694
-
 N-benzyloxycarbonyl-alanyl-prolyl-methionine-thiobenzylester murine granzyme M without bovine serum albumin Homo sapiens
3.4.21.B2 0.695
-
 N-benzyloxycarbonyl-alanyl-aspartyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 0.793
-
 N-benzyloxycarbonyl-alanyl-lysyl-methionine-thiobenzylester murine granzyme M without bovine serum albumin Homo sapiens
3.4.21.B2 0.894
-
 N-benzyloxycarbonyl-lysyl-prolyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 0.92
-
 N-benzyloxycarbonyl-seryl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 1.02
-
 N-benzyloxycarbonyl-alanyl-alanyl-prolyl-methionine-thiobenzylester murine granzyme M without bovine serum albumin Homo sapiens
3.4.21.B2 1.3
-
 N-benzyloxycarbonyl-alanyl-seryl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 2.66
-
 N-benzyloxycarbonyl-alanyl-seryl-methionine-thiobenzylester murine granzyme M without bovine serum albumin Homo sapiens
3.4.21.B2 3.89
-
 N-benzyloxycarbonyl-alanyl-lysyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 6.65
-
 N-benzyloxycarbonyl-seryl-prolyl-methionine-thiobenzylester
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B2 32000 35000 SDS-PAGE, glycosylated forms Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B2 Homo sapiens P51124
-
-
3.4.21.B2 Rattus norvegicus Q03238
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B2 by Ni-affinity chromatography Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.4.21.B2 4°C, Hepes buffer, pH 7.5, several months Homo sapiens
3.4.21.B2 4°C, Hepes buffer, pH 7.5, several months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B2 additional information the enzyme has a strong preference for Pro at the P2 position and Ala, Ser, or Asp at the P3 position Homo sapiens ?
-
 ?
3.4.21.B2 N-benzyloxycarbonyl-alanyl-alanyl-methionine-thiobenzylester + H2O
-
 Homo sapiens N-benzyloxycarbonyl-alanyl-alanyl-methionine + phenylmethanethiol
-
 ?
3.4.21.B2 N-benzyloxycarbonyl-alanyl-alanyl-prolyl-methionine-thiobenzylester + H2O
-
 Homo sapiens N-benzyloxycarbonyl-alanyl-alanyl-prolyl-methionine + phenylmethanethiol
-
 ?
3.4.21.B2 N-benzyloxycarbonyl-alanyl-aspartyl-methionine-thiobenzylester + H2O
-
 Homo sapiens -benzyloxycarbonyl-alanyl-aspartyl-methionine + phenylmethanethiol
-
 ?
3.4.21.B2 N-benzyloxycarbonyl-alanyl-lysyl-methionine-thiobenzylester + H2O
-
 Homo sapiens N-benzyloxycarbonyl-alanyl-lysyl-methionine + phenylmethanethiol
-
 ?
3.4.21.B2 N-benzyloxycarbonyl-alanyl-prolyl-methionine-thiobenzylester + H2O
-
 Homo sapiens N-benzyloxycarbonyl-alanyl-prolyl-methionine + phenylmethanethiol
-
 ?
3.4.21.B2 N-benzyloxycarbonyl-alanyl-seryl-methionine-thiobenzylester + H2O
-
 Homo sapiens N-benzyloxycarbonyl-alanyl-seryl-methionine + phenylmethanethiol
-
 ?
3.4.21.B2 N-benzyloxycarbonyl-aspartyl-prolyl-methionine-thiobenzylester + H2O
-
 Homo sapiens N-benzyloxycarbonyl-aspartyl-prolyl-methionine + phenylmethanethiol
-
 ?
3.4.21.B2 N-benzyloxycarbonyl-L-Phe-L-Pro-L-Met-thiobenzyl ester + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.B2 N-benzyloxycarbonyl-lysyl-prolyl-methionine-thiobenzylester + H2O
-
 Homo sapiens N-benzyloxycarbonyl-lysyl-prolyl-methionine + phenylmethanethiol
-
 ?
3.4.21.B2 N-benzyloxycarbonyl-prolyl-prolyl-methionine-thiobenzylester + H2O
-
 Homo sapiens N-benzyloxycarbonyl-prolyl-prolyl-methionine + phenylmethanethiol
-
 ?
3.4.21.B2 N-benzyloxycarbonyl-seryl-prolyl-methionine-thiobenzylester + H2O
-
 Homo sapiens N-benzyloxycarbonyl-seryl-prolyl-methionine + phenylmethanethiol
-
 ?
3.4.21.B2 N2-Cbz-L-Asp(O-tBut)-L-Pro-L-Met thiobenzyl ester + H2O
-
 Homo sapiens N2-Cbz-L-Asp(O-tBut)-L-Pro-L-Met + phenylmethanethiol
-
 ?
3.4.21.B2 N2-CBZ-O3-tBut-L-Ser-L-Pro-L-Met-SBzl + H2O
-
 Homo sapiens N2-CBZ-O3-tBut-L-Ser-L-Pro-L-Met + phenylmethanethiol
-
 ?
3.4.21.B2 N2-[(benzyloxy)carbonyl]-N6-(tert-butoxycarbonyl)-L-lysyl-L-prolyl-L-methionyl thiobenzyl ester + H2O
-
 Homo sapiens N2-[(benzyloxy)carbonyl]-N6-(tert-butoxycarbonyl)-L-lysyl-L-prolyl-L-methionine + phenylmethanethiol
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.B2 0.00785
-
 N-benzyloxycarbonyl-prolyl-prolyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 0.03
-
 N2-[(benzyloxy)carbonyl]-N6-(tert-butoxycarbonyl)-L-lysyl-L-prolyl-L-methionyl thiobenzyl ester
-
 Homo sapiens
3.4.21.B2 0.031 0.51 N-benzyloxycarbonyl-lysyl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.042
-
 N2-Cbz-L-Asp(O-tBut)-L-Pro-L-Met thiobenzyl ester
-
 Homo sapiens
3.4.21.B2 0.0784
-
 N-benzyloxycarbonyl-phenylalanyl-prolyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 0.0903
-
 N-benzyloxycarbonyl-alanyl-aspartyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 0.0917
-
 N2-CBZ-O3-tBut-L-Ser-L-Pro-L-Met-SBzl
-
 Homo sapiens
3.4.21.B2 0.161
-
 N2-CBZ-O3-tBut-L-Ser-L-Pro-L-Met-SBzl murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.26
-
 N-benzyloxycarbonyl-seryl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.335
-
 N2-[(benzyloxy)carbonyl]-N6-(tert-butoxycarbonyl)-L-lysyl-L-prolyl-L-methionyl thiobenzyl ester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.438
-
 N-benzyloxycarbonyl-alanyl-aspartyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.634
-
 N-benzyloxycarbonyl-phenylalanyl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 0.973
-
 N-benzyloxycarbonyl-prolyl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 1.68
-
 N-benzyloxycarbonyl-alanyl-seryl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 2.13
-
 N2-Cbz-L-Asp(O-tBut)-L-Pro-L-Met thiobenzyl ester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 2.46
-
 N-benzyloxycarbonyl-alanyl-alanyl-methionine-thiobenzylester murine granzyme M without bovine serum albumin Homo sapiens
3.4.21.B2 3.56
-
 N-benzyloxycarbonyl-alanyl-seryl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 4.35
-
 N-benzyloxycarbonyl-alanyl-alanyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 6.07
-
 N-benzyloxycarbonyl-alanyl-lysyl-methionine-thiobenzylester murine granzyme M without bovine serum albumin Homo sapiens
3.4.21.B2 6.08
-
 N-benzyloxycarbonyl-phenylalanyl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 6.08
-
 N-benzyloxycarbonyl-prolyl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 8.01
-
 N-benzyloxycarbonyl-lysyl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 9.68
-
 N-benzyloxycarbonyl-alanyl-lysyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 10.6
-
 N-benzyloxycarbonyl-alanyl-prolyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 10.8
-
 N-benzyloxycarbonyl-alanyl-alanyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 12.3
-
 N-benzyloxycarbonyl-alanyl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 12.8
-
 N-benzyloxycarbonyl-alanyl-seryl-methionine-thiobenzylester murine granzyme M without bovine serum albumin Homo sapiens
3.4.21.B2 13.1
-
 N-benzyloxycarbonyl-alanyl-alanyl-prolyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 13.1
-
 N-benzyloxycarbonyl-lysyl-prolyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 16.1
-
 N-benzyloxycarbonyl-alanyl-alanyl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 17.3
-
 N-benzyloxycarbonyl-aspartyl-prolyl-methionine-thiobenzylester
-
 Homo sapiens
3.4.21.B2 18.1
-
 N-benzyloxycarbonyl-alanyl-prolyl-methionine-thiobenzylester murine granzyme M without bovine serum albumin Homo sapiens
3.4.21.B2 29
-
 N-benzyloxycarbonyl-aspartyl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 30
-
 N-benzyloxycarbonyl-alanyl-alanyl-prolyl-methionine-thiobenzylester murine granzyme M without bovine serum albumin Homo sapiens
3.4.21.B2 52.1
-
 N-benzyloxycarbonyl-seryl-prolyl-methionine-thiobenzylester murine granzyme M with bovine serum albumin Homo sapiens
3.4.21.B2 255
-
 N-benzyloxycarbonyl-seryl-prolyl-methionine-thiobenzylester
-
 Homo sapiens