Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Roma, G.W.; Crowley, L.J.; Barber, M.J.
    Expression and characterization of a functional canine variant of cytochrome b5 reductase (2006), Arch. Biochem. Biophys., 452, 69-82.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.6.2.2 additional information expression of histidine-tagged variant of the soluble, catalytic diaphorase domain, comprising residues I33 to F300. Fragment retains both NADH:ferricyanide reductase and NADH:cytochrome b5 reductase activities Canis lupus familiaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.007
-
 NADH pH 7.0, 25°C Canis lupus familiaris
1.6.2.2 0.008
-
 ferricyanide pH 7.0, 25°C Canis lupus familiaris
1.6.2.2 0.012
-
 ferrocytochrome b5 pH 7.0, 25°C Canis lupus familiaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.2 31365
-
 x * 31365, mass spectrometry of histidine-tagged variant of the soluble, catalytic diaphorase domain, comprising residues I33 to F300 Canis lupus familiaris

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Canis lupus familiaris
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.2 14.9
-
 pH 7.0, 25°C, substrate ferricyanide Canis lupus familiaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricyanide + NADH
-
 Canis lupus familiaris 2 ferrocyanide + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH
-
 Canis lupus familiaris 2 ferrocytochrome b5 + NAD+ + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.2.2 ? x * 31365, mass spectrometry of histidine-tagged variant of the soluble, catalytic diaphorase domain, comprising residues I33 to F300 Canis lupus familiaris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.2 600
-
 ferrocytochrome b5 pH 7.0, 25°C Canis lupus familiaris
1.6.2.2 767
-
 ferricyanide pH 7.0, 25°C Canis lupus familiaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 NADH marked preference for NADH versus NADPH Canis lupus familiaris
1.6.2.2 NADPH marked preference for NADH versus NADPH Canis lupus familiaris