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Literature summary extracted from

  • Shipway, A.; Danahay, H.; Williams, J.A.; Tully, D.C.; Backes, B.J.; Harris, J.L.
    Biochemical characterization of prostasin, a channel activating protease (2004), Biochem. Biophys. Res. Commun., 324, 953-963.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.B6 CHAPS increases enzyme activity up to 4fold with increasing concentration Homo sapiens
3.4.21.B6 DMSO increases enzyme activity up to 2fold with increasing concentration Homo sapiens
3.4.21.B6 additional information NP-40, Triton X-100, 15% glycerol, up to 0.5 mM DTT and 0.25 mM beta-mercaptoethanol have no effect on enzyme activity Homo sapiens
3.4.21.B6 ZnCl2 with acetyl-PRLR-7-amino-3-carbamoylmethyl-4-methylcoumarin Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B6 expression in Sf9 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B6 Aprotinin
-
Homo sapiens
3.4.21.B6 Brij 35 activity decreases with increasing detergent concentration Homo sapiens
3.4.21.B6 CaCl2
-
Homo sapiens
3.4.21.B6 CoCl2
-
Homo sapiens
3.4.21.B6 CuCl2
-
Homo sapiens
3.4.21.B6 EDTA reduces enzyme activity by 30% Homo sapiens
3.4.21.B6 LiCl
-
Homo sapiens
3.4.21.B6 MgCl2
-
Homo sapiens
3.4.21.B6 additional information not inhibited by alpha1-antitrypsin, alpha1-antitrypsinPDX, alpha1-antichymotrypsin, and soybean trypsin inhibitor Homo sapiens
3.4.21.B6 NaCl
-
Homo sapiens
3.4.21.B6 NaF
-
Homo sapiens
3.4.21.B6 NaI
-
Homo sapiens
3.4.21.B6 NiCl2
-
Homo sapiens
3.4.21.B6 placental bikunin
-
Homo sapiens
3.4.21.B6 Tween 20 activity decreases with increasing detergent concentration Homo sapiens
3.4.21.B6 ZnCl2 with acetyl-KHYR-7-amino-3-carbamoylmethyl-4-methylcoumarin Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.B6 0.0555
-
acetyl-KDYR-7-amido-4-carbamoylmethylcoumarin with ZnCL2 Homo sapiens
3.4.21.B6 0.0786
-
acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin without ZnCL2 Homo sapiens
3.4.21.B6 0.141
-
acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin with ZnCL2 Homo sapiens
3.4.21.B6 0.193
-
acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin
-
Homo sapiens
3.4.21.B6 0.219
-
acetyl-KDYR-7-amido-4-carbamoylmethylcoumarin without ZnCL2 Homo sapiens
3.4.21.B6 0.86
-
acetyl-KDYR-7-amido-4-carbamoylmethylcoumarin
-
Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B6 40000
-
SDS-PAGE, glycosylated enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B6 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B6 by gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B6 7-amido-4-carbamoylmethylcoumarin-KHYRSVAS-K(DNP)R + H2O
-
Homo sapiens ?
-
?
3.4.21.B6 7-amido-4-carbamoylmethylcoumarin-KHYRSVAW-K(DNP)R + H2O
-
Homo sapiens ?
-
?
3.4.21.B6 acetyl-DHYR-7-amido-4-carbamoylmethylcoumarin + H2O suboptimal substrate Homo sapiens acetyl-DHYR + 7-amino-4-carbamoylmethylcoumarin
-
?
3.4.21.B6 acetyl-KDYR-7-amido-4-carbamoylmethylcoumarin + H2O suboptimal substrate Homo sapiens acetyl-KDYR + 7-amino-4-carbamoylmethylcoumarin
-
?
3.4.21.B6 acetyl-KHDR-7-amido-4-carbamoylmethylcoumarin + H2O suboptimal substrate Homo sapiens acetyl-KHDR + 7-amino-4-carbamoylmethylcoumarin
-
?
3.4.21.B6 acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin + H2O
-
Homo sapiens acetyl-KHYR + 7-amino-4-carbamoylmethylcoumarin
-
?
3.4.21.B6 acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin + H2O optimal substrate Homo sapiens acetyl-KHYR + 7-amino-4-carbamoylmethylcoumarin
-
?
3.4.21.B6 acetyl-KHYR-7-amido-4-methylcoumarin + H2O
-
Homo sapiens acetyl-KHYR + 7-amino-4-methylcoumarin
-
?
3.4.21.B6 acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin + H2O suboptimal substrate Homo sapiens acetyl-PRLR + 7-amino-4-carbamoylmethylcoumarin
-
?
3.4.21.B6 additional information 7-amido-4-carbamoylmethylcoumarin-KHYRIVAS-K(DNP)R completely inactive Homo sapiens ?
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.B6 0.083
-
acetyl-KDYR-7-amido-4-carbamoylmethylcoumarin
-
Homo sapiens
3.4.21.B6 0.381
-
acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin with ZnCL2 Homo sapiens
3.4.21.B6 0.601
-
acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin without ZnCL2 Homo sapiens
3.4.21.B6 1.97
-
acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin
-
Homo sapiens
3.4.21.B6 2.38
-
acetyl-KDYR-7-amido-4-carbamoylmethylcoumarin without ZnCL2 Homo sapiens
3.4.21.B6 6.08
-
acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin without ZnCL2 Homo sapiens
3.4.21.B6 6.53
-
acetyl-KDYR-7-amido-4-carbamoylmethylcoumarin with ZnCL2 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B6 9
-
-
Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.B6 0.0000014
-
Aprotinin
-
Homo sapiens
3.4.21.B6 0.000005
-
placental bikunin
-
Homo sapiens
3.4.21.B6 0.0021
-
CuCl2 with acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin Homo sapiens
3.4.21.B6 0.0048
-
ZnCl2 with acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin Homo sapiens
3.4.21.B6 0.01
-
NiCl2 with acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin Homo sapiens
3.4.21.B6 0.047
-
CoCl2 with acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin Homo sapiens
3.4.21.B6 0.57
-
CoCl2 with acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin Homo sapiens
3.4.21.B6 1.15
-
CaCl2 with acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin Homo sapiens
3.4.21.B6 1.42
-
MgCl2 with acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin Homo sapiens
3.4.21.B6 16.4
-
NaI with acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin Homo sapiens
3.4.21.B6 21.3
-
LiCl with acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin Homo sapiens
3.4.21.B6 22.4
-
NaCl with acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin Homo sapiens
3.4.21.B6 26.3
-
NaF with acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin Homo sapiens