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Literature summary extracted from

  • Hassan-Abdallah, A.; Bruckner, R.C.; Zhao, G.; Jorns, M.S.
    Biosynthesis of covalently bound flavin: isolation and in vitro flavinylation of the monomeric sarcosine oxidase apoprotein (2005), Biochemistry, 44, 6452-6462.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.5.3.1 additional information production of soluble apoenzyme lacking covalently bound FAD by controlled expression in Escherichia coli. Reconstitution of enzyme by incubation with FAD Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.1 Bacillus sp. (in: Bacteria)
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.5.3.1 production of soluble apoenzyme lacking FAD by controlled expression in Escherichia coli. Reconstitution of enzyme by incubation with FAD. Autoflavinylation occurs in a reaction that proceeds via reduced flavin intermediate and requires only apoenzyme and FAD Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.3.1 1.1
-
 apoenzyme lacking FAD Bacillus sp. (in: Bacteria)
1.5.3.1 3
-
 apoenzyme reconstituted with 8-nor-8-chloro-FAD Bacillus sp. (in: Bacteria)
1.5.3.1 27
-
 apoenzyme reconstituted with FAD Bacillus sp. (in: Bacteria)
1.5.3.1 34.7
-
 native enzyme Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.1 sarcosine + H2O + O2
-
 Bacillus sp. (in: Bacteria) glycine + formaldehyde + H2O2
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.1 FAD covalently bound to wild-type. Production of soluble apoenzyme lacking FAD by controlled expression in Escherichia coli. Reconstitution of enzyme by incubation with FAD. Autoflavinylation occurs in a reaction that proceeds via reduced flavin intermediate and requires only apoenzyme and FAD Bacillus sp. (in: Bacteria)