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Literature summary extracted from

  • Purpero, V.M.; Moran, G.R.
    Catalytic, noncatalytic, and inhibitory phenomena: kinetic analysis of (4-hydroxyphenyl)pyruvate dioxygenase from Arabidopsis thaliana (2006), Biochemistry, 45, 6044-6055.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.27 expressed in Escherichia coli BL21 DE3 Arabidopsis thaliana

General Stability

EC Number General Stability Organism
1.13.11.27 the enzyme is stable for several hours at room temperature, indefinitely at 4°C Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.27 2-(2-nitro-4-(trifluoromethyl)benzoyl)-1,3-cyclohexanedione (NTBC), binding constant of 937 microM Arabidopsis thaliana
1.13.11.27 mesotrione binding constant of 602 microM Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.27 additional information
-
additional information Kd value for 4-hydroxyphenylpyruvate with 4-hydroxyphenylpyruvate dioxygenase Fe2+ is 29 microM, whereas the binding constant for phenylpyruvate is determined to be 319 microM Arabidopsis thaliana
1.13.11.27 0.0069
-
4-hydroxyphenylpyruvate activity is measured polarographically, assays include 0.5 microM of enzyme, 500 microM betaME, Fe2+ stoichiometric with enzyme, and 400 microM 4-hydroxyophenylpyruvate in 20 mM HEPES at pH 7.0 at 4°C Arabidopsis thaliana
1.13.11.27 0.115
-
oxygen activity is measured polarographically, assays include 0.5 microM of enzyme, 500 microM betaME, Fe2+ stoichiometric with enzyme, and 400 microM 4-hydroxyophenylpyruvate in 20 mM HEPES at pH 7.0 at 4°C Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.27 48650
-
-
Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.27 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.27 Q Sepharose anion exchange column, Sephacryl S-200 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.27 4-hydroxyphenylpyruvate + O2 4-hydroxyphenylpyruvate dioxygenase does not use phenolpyruvate as a substrate in normal plant metabolism Arabidopsis thaliana homogentisate + CO2
-
?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.27 HPPD
-
Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.27 0.065
-
4-hydroxyphenylpyruvate activity is measured polarographically, assays include 0.5 microM of enzyme, 500 microM betaME, Fe2+ stoichiometric with enzyme, and 400 microM 4-hydroxyophenylpyruvate in 20 mM HEPES at pH 7.0 at 4°C Arabidopsis thaliana