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Literature summary extracted from

  • Serebryakova, L.T.; Sheremetieva, M.E.
    Characterization of catalytic properties of hydrogenase isolated from the unicellular cyanobacterium Gloeocapsa alpicola CALU 743 (2006), Biochemistry, 71, 1370-1376.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.1.2 0.0007
-
 NADPH 30°C, pH 7.0 Gloeocapsa alpicola
1.12.1.2 0.0023
-
 NADH 30°C, pH 7.0 Gloeocapsa alpicola
1.12.1.2 0.0035
-
 NAD+ 30°C, pH 8.5 Gloeocapsa alpicola
1.12.1.2 0.0306
-
 reduced methyl viologen 30°C, pH 7.0 Gloeocapsa alpicola
1.12.1.2 0.0375
-
 oxidized methyl viologen 30°C, pH 8.5 Gloeocapsa alpicola
1.12.1.2 0.0578
-
 Oxidized benzyl viologen 30°C, pH 8.5 Gloeocapsa alpicola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.1.2 additional information Gloeocapsa alpicola physiological role of the hydrogenase seems to be equally associated with both formation and uptake of H2, depending on the redox state of the intracellular medium ?
-
 ?
1.12.1.2 additional information Gloeocapsa alpicola CALU 743 physiological role of the hydrogenase seems to be equally associated with both formation and uptake of H2, depending on the redox state of the intracellular medium ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.2 Gloeocapsa alpicola
-
-
-
1.12.1.2 Gloeocapsa alpicola CALU 743
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.1.2
-
 Gloeocapsa alpicola

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.1.2 9.8
-
-
 Gloeocapsa alpicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.2 H2 + NAD+ only 3-8% of the benzyl biologen or methyl viologen-dependent values. The enzyme interacts with NADP+ and NADPH, but is more specific to NAD+ and NADH Gloeocapsa alpicola H+ + NADH
-
 r
1.12.1.2 H2 + NAD+ only 3-8% of the benzyl biologen or methyl viologen-dependent values. The enzyme interacts with NADP+ and NADPH, but is more specific to NAD+ and NADH Gloeocapsa alpicola CALU 743 H+ + NADH
-
 r
1.12.1.2 H2 + NADP+ only 3-8% of the benzyl biologen or methyl viologen-dependent values. The enzyme interacts with NADP+ and NADPH, but is more specific to NAD+ and NADH Gloeocapsa alpicola H+ + NADPH
-
 r
1.12.1.2 H2 + NADP+ only 3-8% of the benzyl biologen or methyl viologen-dependent values. The enzyme interacts with NADP+ and NADPH, but is more specific to NAD+ and NADH Gloeocapsa alpicola CALU 743 H+ + NADPH
-
 r
1.12.1.2 H2 + oxidized benzyl viologen
-
 Gloeocapsa alpicola reduced benzyl viologen + H+
-
 ?
1.12.1.2 H2 + oxidized benzyl viologen
-
 Gloeocapsa alpicola CALU 743 reduced benzyl viologen + H+
-
 ?
1.12.1.2 H2 + oxidized methyl viologen
-
 Gloeocapsa alpicola reduced methyl viologen + H+
-
 r
1.12.1.2 H2 + oxidized methyl viologen
-
 Gloeocapsa alpicola CALU 743 reduced methyl viologen + H+
-
 r
1.12.1.2 additional information physiological role of the hydrogenase seems to be equally associated with both formation and uptake of H2, depending on the redox state of the intracellular medium Gloeocapsa alpicola ?
-
 ?
1.12.1.2 additional information physiological role of the hydrogenase seems to be equally associated with both formation and uptake of H2, depending on the redox state of the intracellular medium Gloeocapsa alpicola CALU 743 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.12.1.2 Hox type hydrogenase
-
 Gloeocapsa alpicola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.1.2 45
-
 evolution of H2 in the presence of NADH or NADPH Gloeocapsa alpicola
1.12.1.2 50
-
 evolution of H2 in the presence of NADH or NADPH Gloeocapsa alpicola
1.12.1.2 65
-
 evolution of H2 in the presence of reduced methyl viologen Gloeocapsa alpicola
1.12.1.2 70
-
 evolution of H2 in the presence of methyl viologen or benzyl viologen Gloeocapsa alpicola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.1.2 4.3
-
 evolution of H2 in the presence of reduced methyl viologen Gloeocapsa alpicola
1.12.1.2 6.8
-
 evolution of H2 in the presence of NADH or NADPH Gloeocapsa alpicola
1.12.1.2 8.5
-
 evolution of H2 in the presence of NADH or NADPH Gloeocapsa alpicola
1.12.1.2 9
-
 evolution of H2 in the presence of methyl viologen or benzyl viologen Gloeocapsa alpicola