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Literature summary extracted from

  • Rakhmanova, T.I.; Popova, T.N.
    Regulation of 2-oxoglutarate metabolism in rat liver by NADP-isocitrate dehydrogenase and aspartate aminotransferase (2006), Biochemistry, 71, 211-217.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.1 aconitate activating effect on the activity of the cytoplasmic enzyme form Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.1 fumarate
-
 Rattus norvegicus
2.6.1.1 isocitrate
-
 Rattus norvegicus
2.6.1.1 L-aspartate
-
 Rattus norvegicus
2.6.1.1 L-glutamate
-
 Rattus norvegicus
2.6.1.1 L-glutamine
-
 Rattus norvegicus
2.6.1.1 succinate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.1 0.35
-
 L-aspartate cytoplasmic enzyme Rattus norvegicus
2.6.1.1 0.75
-
 L-aspartate mitochondrial enzyme Rattus norvegicus
2.6.1.1 1.25
-
 2-oxoglutarate cytoplasmic enzyme Rattus norvegicus
2.6.1.1 1.75
-
 2-oxoglutarate mitochondrial enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.1 cytoplasm
-
 Rattus norvegicus 5737
-
2.6.1.1 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.1 92000
-
 mitochondrial form, SDS-PAGE Rattus norvegicus
2.6.1.1 98000
-
 cytoplasmic form, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1 differential centrifugation, Sephadex G-25 gel filtration, DEAE cellulose ion exchange chromatography, and Sephadex G-150 gel filtration Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.1 22.37
-
 mitochondrial enzyme Rattus norvegicus
2.6.1.1 48.45
-
 cytoplasmic enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Rattus norvegicus oxaloacetate + L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.1 AsAT
-
 Rattus norvegicus
2.6.1.1 aspartate aminotransferase
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.1 6.8
-
 mitochondrial form Rattus norvegicus
2.6.1.1 7.5
-
 cytoplasmic form Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.1 1.6
-
 L-aspartate cytoplasmic enzyme Rattus norvegicus
2.6.1.1 1.8
-
 L-glutamate mitochondrial enzyme Rattus norvegicus
2.6.1.1 1.9
-
 L-aspartate mitochondrial enzyme Rattus norvegicus
2.6.1.1 2.4
-
 L-glutamate cytoplasmic enzyme Rattus norvegicus
2.6.1.1 2.6
-
 L-glutamine mitochondrial enzyme Rattus norvegicus
2.6.1.1 3.7
-
 L-glutamine cytoplasmic enzyme Rattus norvegicus
2.6.1.1 4.1
-
 succinate mitochondrial enzyme Rattus norvegicus
2.6.1.1 5.7
-
 succinate cytoplasmic enzyme Rattus norvegicus
2.6.1.1 6.6
-
 isocitrate cytoplasmic enzyme Rattus norvegicus
2.6.1.1 8.4
-
 fumarate cytoplasmic enzyme Rattus norvegicus