Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wielgus-Kutrowska, B.; Bzowska, A.
    Probing the mechanism of purine nucleoside phosphorylase by steady-state kinetic studies and ligand binding characterization determined by fluorimetric titrations (2006), Biochim. Biophys. Acta, 1764, 887-902.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 2-amino-6-oxo-9-[3-hydroxy-2-(phosphonomethoxy)propyl]-purine
-
 Cellulomonas sp.
2.4.2.1 2-amino-9-[2-(phosphonomethoxy)ethyl]-6-sulfanylpurine
-
 Cellulomonas sp.
2.4.2.1 7-methylguanine
-
 Cellulomonas sp.
2.4.2.1 8-aza-2,6-diamino-(S)-9-[2-(phosphonomethoxy)ethyl]-purine
-
 Cellulomonas sp.
2.4.2.1 alpha-D-ribose 1-phosphate mixed inhibition of phosphorolysis of inosine Cellulomonas sp.
2.4.2.1 guanine competitive inhibition of hydrolysis of inosine Cellulomonas sp.
2.4.2.1 guanosine uncompetitive with alpha-D-1-ribose 1-phosphate as varied substrate Cellulomonas sp.
2.4.2.1 phosphate
-
 Cellulomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.0264
-
 7-methylguanosine 25°C, pH 7.0 Cellulomonas sp.
2.4.2.1 0.0343
-
 phosphate 25°C, pH 7.0, cosubstrate: 7-methylguanosine Cellulomonas sp.
2.4.2.1 0.132
-
 phosphate 25°C, pH 7.0, cosubstrate: inosine Cellulomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Cellulomonas sp. P81989
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.1 purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate mechanism: 1. random binding of substrates, 2. potent binding and slow release of some reaction products leading to the circumstances that the chamical step is not the slowest one and that rapid-equilibrium assumptions do not hold, 3. dual role of phosphate, as substrate and as reaction modifier Cellulomonas sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 7-methylguanosine + phosphate
-
 Cellulomonas sp. 7-methylguanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Cellulomonas sp. hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.1 0.0025
-
 guanine 25°C, pH 7.0, with phosphate as varied substrate Cellulomonas sp.
2.4.2.1 0.0036
-
 guanine 25°C, pH 7.0, with inosine as varied substrate Cellulomonas sp.
2.4.2.1 0.082
-
 7-methylguanine 25°C, pH 7.0, with 7-methylguanosine as varied substrate Cellulomonas sp.
2.4.2.1 0.8
-
 guanosine 25°C, pH 7.0, about, with alpha-D-ribose 1-phosphate as varied substrate Cellulomonas sp.
2.4.2.1 2.5
-
 phosphate 25°C, pH 7.0, with alpha-D-ribose 1-phosphate as varied substrate Cellulomonas sp.
2.4.2.1 4.7
-
 phosphate 25°C, pH 7.0, with guanine as varied substrate Cellulomonas sp.