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Literature summary extracted from

  • Song, L.; Wang, M.; Yang, X.; Qian, S.
    Purification and characterization of the enantioselective nitrile hydratase from Rhodococcus sp. AJ270 (2007), Biotechnol. J., 2, 717-724.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.84 AgNO3 1 mM, complete inhibition of the enzyme Rhodococcus sp.
4.2.1.84 ammonium persulfate 1 mM, relative activity: 0.3% Rhodococcus sp.
4.2.1.84 CuCl2 1 mM, relative activity: 4.8% Rhodococcus sp.
4.2.1.84 EDTA 1 mM, relative activity: 96.2% Rhodococcus sp.
4.2.1.84 hydrogen peroxide 1 mM, relative activity: 0% Rhodococcus sp.
4.2.1.84 iodoacetamide 1 mM, relative activity: 68.6% Rhodococcus sp.
4.2.1.84 iodoacetic acid 1 mM, relative activity: 93.0% Rhodococcus sp.
4.2.1.84 LiCl 1 mM, relative activity: 97.3% Rhodococcus sp.
4.2.1.84 MgCl2 1 mM, relative activity: 73.7% Rhodococcus sp.
4.2.1.84 N-bromosuccinimide 1 mM, relative activity: 0.2% Rhodococcus sp.
4.2.1.84 Sodium azide 1 mM, relative activity: 61.3% Rhodococcus sp.
4.2.1.84 ZnCl2 1 mM, relative activity: 77.6% Rhodococcus sp.
4.2.1.84 ZnSO4 1 mM, relative activity: 99.5% Rhodococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.84 BaCl2 1 mM, relative activity: 101.1% Rhodococcus sp.
4.2.1.84 CaCl2 1 mM, relative activity: 102.0% Rhodococcus sp.
4.2.1.84 CoCl2 1 mM, relative activity: 106.4% Rhodococcus sp.
4.2.1.84 Na2MoO4 1 mM, relative activity: 102.1% Rhodococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 22975
-
 alphabeta, 1 * 22975 + 1 * 23493, MALDI-TOF MS Rhodococcus sp.
4.2.1.84 23493
-
 alphabeta, 1 * 22975 + 1 * 23493, MALDI-TOF MS Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Rhodococcus sp.
-
-
-
4.2.1.84 Rhodococcus sp. AJ270
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.84 The enzyme is strongly inhibited by oxidizing agents like H2O2, ammonium persulfate and N-bromosuccinimide. Rhodococcus sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84 (NH4)2SO4 fractionation, ion-exchange/hydrophobic/gel-filtration chromatography, stabilization by n-butyric acid, yield of purified protein: 23.96% Rhodococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 additional information
-
 no conversion of KCN Rhodococcus sp.
4.2.1.84 829.5
-
 purified protein Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 (1R,3R)-2,2-dibromo-3-phenylcyclopropanecarbonitrile + H2O substrate conversion: 11.6%, enantiomeric excess: 83.8, 88.9 (methanol), 81.0 (n-hexane) Rhodococcus sp. (1R,3R)-2,2-dibromo-3-phenylcyclopropanecarbamide
-
 r
4.2.1.84 (1R,3R)-2,2-dibromo-3-phenylcyclopropanecarbonitrile + H2O substrate conversion: 11.6%, enantiomeric excess: 83.8, 88.9 (methanol), 81.0 (n-hexane) Rhodococcus sp. AJ270 (1R,3R)-2,2-dibromo-3-phenylcyclopropanecarbamide
-
 r
4.2.1.84 (1R,3R)-3-phenylcyclopropanecarbonitrile + H2O substrate conversion: 49.1%, enantiomeric excess: 22.7, 31.1 (methanol), 21.6 (n-hexane) Rhodococcus sp. (1R,3R)-3-phenylcyclopropanecarbamide
-
 r
4.2.1.84 (1R,3R)-3-phenylcyclopropanecarbonitrile + H2O substrate conversion: 49.1%, enantiomeric excess: 22.7, 31.1 (methanol), 21.6 (n-hexane) Rhodococcus sp. AJ270 (1R,3R)-3-phenylcyclopropanecarbamide
-
 r
4.2.1.84 (1R,3S)-3-phenylcyclopropanecarbonitrile + H2O substrate conversion: 25.8%, enantiomeric excess: 95.4, 95.4 (methanol), 95.5 (n-hexane) Rhodococcus sp. (1R,3S)-3-phenylcyclopropanecarbamide
-
 r
4.2.1.84 (1R,3S)-3-phenylcyclopropanecarbonitrile + H2O substrate conversion: 25.8%, enantiomeric excess: 95.4, 95.4 (methanol), 95.5 (n-hexane) Rhodococcus sp. AJ270 (1R,3S)-3-phenylcyclopropanecarbamide
-
 r
4.2.1.84 (1S,3S)-2,2-dimethyl-3-phenylcyclopropanecarbonitrile + H2O substrate conversion: 40.3%, enantiomeric excess: 84.7 Rhodococcus sp. (1S,3S)-2,2-dimethyl-3-phenylcyclopropanecarbamide
-
 r
4.2.1.84 (1S,3S)-2,2-dimethyl-3-phenylcyclopropanecarbonitrile + H2O substrate conversion: 7.9%, enantiomeric excess: 3.2, 5.9 (methanol), 0.7 (n-hexane) Rhodococcus sp. (1S,3S)-2,2-dimethyl-3-phenylcyclopropanecarbamide
-
 r
4.2.1.84 (S)-3-benzoyloxypentanedinitrile + H2O substrate conversion: 38.5%, enantiomeric excess: 68.2 Rhodococcus sp. 3-amino-1-(2-amino-2-oxoethyl)-3-oxopropyl benzoate
-
 r
4.2.1.84 1-(4-bromo-phenyl)-aziridine-2-carbonitrile + H2O
-
 Rhodococcus sp. 1-(4-bromophenyl)aziridine-2-carboxamide
-
 r
4.2.1.84 1-(4-methoxy-phenyl)-aziridine-2-carbonitrile + H2O
-
 Rhodococcus sp. 1-(4-methoxyphenyl)aziridine-2-carboxamide
-
 r
4.2.1.84 2,3-dihydro-benzo(1,4)dioxine-2-carbonitrile + H2O substrate conversion: 45.1%, enantiomeric excess: 0 Rhodococcus sp. 2,3-dihydro-1,4-benzodioxine-2-carboxamide
-
 r
4.2.1.84 2-hydroxymethyl-3-phenyl-propionitrile + H2O
-
 Rhodococcus sp. 2-benzyl-3-hydroxypropanamide
-
 r
4.2.1.84 2-methoxymethyl-3-phenyl-propionitrile + H2O
-
 Rhodococcus sp. 2-benzyl-3-methoxypropanamide
-
 r
4.2.1.84 3-allyloxy-4-phenyl-butyronitrile + H2O
-
 Rhodococcus sp. 4-phenyl-3-(prop-2-en-1-yloxy)butanamide
-
 r
4.2.1.84 3-benzyloxy-3-vinyl-propionitrile + H2O
-
 Rhodococcus sp. 3-(benzyloxy)pent-4-enamide
-
 r
4.2.1.84 3-benzyloxy-pentanonitrile + H2O
-
 Rhodococcus sp. 3-(benzyloxy)pentanamide
-
 r
4.2.1.84 acrylonitrile + H2O
-
 Rhodococcus sp. acrylamide
-
 r
4.2.1.84 acrylonitrile + H2O
-
 Rhodococcus sp. AJ270 acrylamide
-
 r
4.2.1.84 alpha-methylbenzyl cyanide + H2O
-
 Rhodococcus sp. 2-phenylpropanamide
-
 r
4.2.1.84 benzylcyanide + H2O
-
 Rhodococcus sp. 2-phenylacetamide
-
 r
4.2.1.84 n-butyronitrile + H2O
-
 Rhodococcus sp. n-butyramide
-
 r
4.2.1.84 propionitrile + H2O
-
 Rhodococcus sp. propionamide
-
 r
4.2.1.84 propionitrile + H2O
-
 Rhodococcus sp. AJ270 propionamide
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.84 heterodimer alphabeta, 1 * 22975 + 1 * 23493, MALDI-TOF MS Rhodococcus sp.

Synonyms

EC Number Synonyms Comment Organism
4.2.1.84 NHase
-
 Rhodococcus sp.
4.2.1.84 nitrile hydratase
-
 Rhodococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.84 25
-
 hydration of acrylonitrile Rhodococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.84 7.2 7.6 hydration of acrylonitrile at 25°C, rapid decrease of activity below pH 6.4 Rhodococcus sp.