Literature summary extracted from

Hao, J.; Ma, C.; Gao, C.; Qiu, J.; Wang, M.; Zhang, Y.; Cui, X.; Xu, P.
Pseudomonas stutzeri as a novel biocatalyst for pyruvate production from DL-lactate (2007), Biotechnol. Lett., 29, 105-110.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
1.1.2.4	additional information	the enzyme is induced by lacate as sole carbon source during growth	Pseudomonas stutzeri

Application

EC Number	Application	Comment	Organism
1.1.2.4	synthesis	Pseudomonas stutzeri containing D-lactate dehydrogenase can act as a novel biocatalyst for pyruvate production from DL-lactate	Pseudomonas stutzeri

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.1.2.3	45390	-	-	Pseudomonas stutzeri

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.2.3	Pseudomonas stutzeri	-	-	-
1.1.2.4	Pseudomonas stutzeri	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.1.2.4	culture condition:D-lactate-grown cell	D-lactate as sole carbon source	Pseudomonas stutzeri	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.2.4	D-lactate + 2,6-dichlorophenolindophenol	i.e. DCPIP, via cofactor FAD, stereospecific enzyme	Pseudomonas stutzeri	pyruvate + reduced 2,6-dichlorophenolindophenol	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.2.3	iLDH	-	Pseudomonas stutzeri
1.1.2.3	L-LDH (FMN-dependent)	-	Pseudomonas stutzeri
1.1.2.3	NAD+ - independent LDH	-	Pseudomonas stutzeri
1.1.2.4	iLDH	-	Pseudomonas stutzeri
1.1.2.4	NAD+-independent LDH	-	Pseudomonas stutzeri

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.1.2.4	30	-	assay at	Pseudomonas stutzeri

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.2.4	7.5	-	assay at	Pseudomonas stutzeri

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.2.4	FAD	bound to the FAD binding domain of the enzyme	Pseudomonas stutzeri

pI Value

EC Number	Organism	Comment	pI Value Maximum	pI Value
1.1.2.3	Pseudomonas stutzeri	-	-	5.1

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)