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Literature summary extracted from

  • Butcher, N.J.; Tetlow, N.L.; Cheung, C.; Broadhurst, G.M.; Minchin, R.F.
    Induction of human arylamine N-acetyltransferase type I by androgens in human prostate cancer cells (2007), Cancer Res., 67, 85-92.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.5 additional information the androgen R1881 induces the enzyme about 4.5fold in androgen receptor AR-positive prostate cell lines 22Rv1 and LNCaP, but not in the AR-negative PC-3, HK-293, or HeLa cells, androgen up-regulation of NAT1 is prevented by the AR antagonist flutamide, the effect of R1881 may not be by direct transcriptional activation of NAT1, overview Homo sapiens
2.3.1.5 R1881 the androgen R1881 induces NAT1 activity in androgen receptor-positive prostate cancer cells Homo sapiens

Application

EC Number Application Comment Organism
2.3.1.5 medicine it is shown that human NAT1 is induced by androgens, which may have implications for cancer risk in individuals Homo sapiens
2.3.1.5 medicine results show that human NAT1 is induced by androgens, which may have implications for cancer risk in individuals Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.5 a series of constructs is cloned into the pGL3-enhancer Homo sapiens
2.3.1.5 gene NAT1, DNA and amino acid sequence determnination and analysis, quantitative expression analysis Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.5 cytoplasm NAT1 expression is detected in the cytoplasm as well as in the nucleus Homo sapiens 5737
-
2.3.1.5 nucleus NAT1 expression is detected in the cytoplasm as well as in the nucleus Homo sapiens 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.5 33000
-
 SDS-PAGE Homo sapiens
2.3.1.5 33000
-
 determined by SDS-PAGE and Western Blot analysis Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.5 acetyl-CoA + an arylamine Homo sapiens
-
 CoA + an N-acetylarylamine
-
 ?
2.3.1.5 additional information Homo sapiens the human enzyme NAT1 bioactivates arylamine and heterocyclic amine carcinogens present in red meat and tobacco products, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Homo sapiens
-
-
-
2.3.1.5 Homo sapiens P18440 isozyme NAT1; gene NAT1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.5 22Rv1 cell
-
 Homo sapiens
-
2.3.1.5 22Rv1 cell a prostate cancer cell line Homo sapiens
-
2.3.1.5 22Rv1 cell NAT1 expression is up-regulated by synthetic androgen R1881 in androgen receptor (AR)-positive prostate lines 22Rv1. Androgen up-regulation of NAT1 is prevented by the androgen antagonist flutamide Homo sapiens
-
2.3.1.5 HEK-293 cell
-
 Homo sapiens
-
2.3.1.5 HeLa cell
-
 Homo sapiens
-
2.3.1.5 LNCaP cell
-
 Homo sapiens
-
2.3.1.5 LNCaP cell a prostate cancer cell line Homo sapiens
-
2.3.1.5 PC-3 cell
-
 Homo sapiens
-
2.3.1.5 prostate gland NAT1 is strongly localized to androgen-positive epithelium in human prostate Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.5 additional information
-
 it is shown that NAT1 activity is induced by synthetic androgen R1881 in androgen receptor (AR)-positive prostate lines 22Rv1 and LNCaP, but not in the AR-negative PC-3, HK-293, or HeLa cells. The effect of R1881 is dose dependent, with an EC50 for R1881 of 1.6 nmol/L Homo sapiens
2.3.1.5 0.01
-
 HEK-293 cell, treated with R1881 Homo sapiens
2.3.1.5 0.01
-
 PC-3 cell Homo sapiens
2.3.1.5 0.0104
-
 effect of androgen receptor expression in PC-3 cells, control, plus R1881 Homo sapiens
2.3.1.5 0.0109
-
 effect of androgen receptor expression in PC-3 cells, plus pCMV-AR3.1, plus R1881 Homo sapiens
2.3.1.5 0.011
-
 HEK-293 cell Homo sapiens
2.3.1.5 0.011
-
 PC-3 cell, treated with R1881 Homo sapiens
2.3.1.5 0.0113
-
 effect of androgen receptor expression in PC-3 cells, control Homo sapiens
2.3.1.5 0.0115
-
 effect of androgen receptor expression in PC-3 cells, plus pCMV-AR3.1 Homo sapiens
2.3.1.5 0.014
-
 HeLa cell Homo sapiens
2.3.1.5 0.015
-
 HeLa cell, treated with R1881 Homo sapiens
2.3.1.5 0.038
-
 22Rv1 cell, plus R1881, plus flutamide Homo sapiens
2.3.1.5 0.04
-
 22Rv1 cell, 24h Homo sapiens
2.3.1.5 0.04
-
 22Rv1 cell, 48h Homo sapiens
2.3.1.5 0.04
-
 22Rv1 cell, plus bicalutamide, 24h Homo sapiens
2.3.1.5 0.04
-
 22Rv1 cell, plus bicalutamide, 48h Homo sapiens
2.3.1.5 0.04
-
 22Rv1 cell, plus R1881, plus bicalutamide Homo sapiens
2.3.1.5 0.04
-
 22Rv1 cell, plus R1881, plus bicalutamide, 24h Homo sapiens
2.3.1.5 0.042
-
 22Rv1 cell, plus DMSO Homo sapiens
2.3.1.5 0.045
-
 LNCaP cell Homo sapiens
2.3.1.5 0.046
-
 22Rv1 cells and LNCAP cells (both androgene-positive lines) show a high basal level of NAT1 activity between 46-51 nmol/min/mg protein Homo sapiens
2.3.1.5 0.05
-
 22Rv1 cell Homo sapiens
2.3.1.5 0.07
-
 22Rv1 cell, plus R1881 Homo sapiens
2.3.1.5 0.07
-
 LNCaP cell, treated with R1881 Homo sapiens
2.3.1.5 0.08
-
 22Rv1 cell, plus R1881, 24h Homo sapiens
2.3.1.5 0.13
-
 22Rv1 cell, treated with R1881 Homo sapiens
2.3.1.5 0.14
-
 22Rv1 prostate cells upon treatment with 100 nmol of synthetic androgen R1881 for 24h Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 acetyl-CoA + an arylamine
-
 Homo sapiens CoA + an N-acetylarylamine
-
 ?
2.3.1.5 additional information the human enzyme NAT1 bioactivates arylamine and heterocyclic amine carcinogens present in red meat and tobacco products, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.5 arylamine N-acetyltransferase
-
 Homo sapiens
2.3.1.5 arylamine N-acetyltransferase type I
-
 Homo sapiens
2.3.1.5 NAT1
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.5 7.4
-
 assay at Homo sapiens
2.3.1.5 7.4
-
 activity assay Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.5 acetyl-CoA
-
 Homo sapiens