EC Number | Application | Comment | Organism |
---|---|---|---|
2.3.1.164 | biotechnology | Penicillium chrysogenum npe6 lacking isopenicillin N acyltransferase activity is an excellent host for production of different beta-lactam antibiotics | Penicillium chrysogenum |
2.3.1.175 | biotechnology | Penicillium chrysogenum npe6 lacking isopenicillin N acyltransferase activity is an excellent host for production of different beta-lactam antibiotics | Acremonium chrysogenum |
2.3.1.175 | synthesis | production of acetylcephalosporin C by gene expression in Penicillium chrysogenum. Recombinant strains secrete significant amounts of deacetylcephalosporin C, but acetylcephalosporin C is not detected in culture broth. Even when accumulating intracellularly, acetylcephalosporin C is not found extracellularly | Acremonium chrysogenum |
5.1.1.17 | biotechnology | The results indicate that Penecillium chrysogenum can be used as heterologous host for the production of deacetylcephalosporin C | Acremonium chrysogenum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.175 | Penicillium chrysogenum strains expressing cefD1, cefD2, cefEF, and cefG genes cloned form Acremonium chrysogenum are constructed, deacetylcephalosporin-C acetyltransferase is coded by the cefG gene | Acremonium chrysogenum |
5.1.1.17 | Penecillium chrysogenum strains are constructed expressing cefD1 (isopenecillin N-CoA synthetase), cefD2 (isopenecillin N-CoA epimerase), cefEF (deacetylcephalosporin-actyltransferase), and cefG (deacetylcephalosporin C synthetase) genes cloned from Acremonium chrysogenum. HPLC analysis of cell extracts show that transformant TA64, TA71, and TA98 accumulate intracellularly deacetylcephalosporin C and, in the last strain (TA98), also cephalosporin C. | Acremonium chrysogenum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.175 | protoplast | - |
Acremonium chrysogenum | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.164 | phenylacetyl-CoA + isopenicillin N + H2O | Penicillium chrysogenum | - |
CoA + penicillin G + L-2-aminohexanedioate | - |
? | |
2.3.1.175 | acetyl-CoA + deacetylcephalosporin C | Acremonium chrysogenum | - |
CoA + cephalosporin C | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.164 | Penicillium chrysogenum | - |
Penicillium chrysogenum npe6 lacks isopenicillin N acyltransferase activity | - |
2.3.1.175 | Acremonium chrysogenum | - |
- |
- |
2.3.1.175 | Acremonium chrysogenum | - |
expression in Penicillium chrysogenum | - |
5.1.1.17 | Acremonium chrysogenum | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.175 | 6.7 | - |
deacetylcephalosporin-C acetyltransferase activity in the host strain Penicillium chrysogenum TA64 | Acremonium chrysogenum |
2.3.1.175 | 7.2 | - |
deacetylcephalosporin-C acetyltransferase activity in the host strain Penicillium chrysogenum TA71 | Acremonium chrysogenum |
2.3.1.175 | 25.8 | - |
deacetylcephalosporin-C acetyltransferase activity in the host strain Penicillium chrysogenum TA2 | Acremonium chrysogenum |
2.3.1.175 | 30 | - |
deacetylcephalosporin-C acetyltransferase activity in the host strain Penicillium chrysogenum TA98 | Acremonium chrysogenum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.164 | phenylacetyl-CoA + isopenicillin N + H2O | - |
Penicillium chrysogenum | CoA + penicillin G + L-2-aminohexanedioate | - |
? | |
2.3.1.175 | acetyl-CoA + deacetylcephalosporin C | - |
Acremonium chrysogenum | CoA + cephalosporin C | - |
? | |
5.1.1.17 | isopenicillin N | - |
Acremonium chrysogenum | penicillin N | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.164 | isopenicillin N acyltransferase | - |
Penicillium chrysogenum |
2.3.1.164 | isopenicillin-N N-acyltransferase | - |
Penicillium chrysogenum |
2.3.1.175 | acetyl-CoA:DAC-acetyltransferase | - |
Acremonium chrysogenum |
2.3.1.175 | deacetylcephalosporin-C acetyltransferase | - |
Acremonium chrysogenum |
5.1.1.17 | isopenecillin N-CoA epimerase | - |
Acremonium chrysogenum |
5.1.1.17 | isopenicillin N epimerase | - |
Acremonium chrysogenum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.175 | 37 | - |
activity assay | Acremonium chrysogenum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.175 | 7 | - |
activity assay | Acremonium chrysogenum |