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Literature summary extracted from

  • Ullan, R.V.; Campoy, S.; Casqueiro, J.; Fernandez, F.J.; Martin, J.F.
    Deacetylcephalosporin C production in Penicillium chrysogenum by expression of the isopenicillin N epimerization, ring expansion, and acetylation genes (2007), Chem. Biol., 14, 329-339.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.164 biotechnology Penicillium chrysogenum npe6 lacking isopenicillin N acyltransferase activity is an excellent host for production of different beta-lactam antibiotics Penicillium chrysogenum
2.3.1.175 biotechnology Penicillium chrysogenum npe6 lacking isopenicillin N acyltransferase activity is an excellent host for production of different beta-lactam antibiotics Acremonium chrysogenum
2.3.1.175 synthesis production of acetylcephalosporin C by gene expression in Penicillium chrysogenum. Recombinant strains secrete significant amounts of deacetylcephalosporin C, but acetylcephalosporin C is not detected in culture broth. Even when accumulating intracellularly, acetylcephalosporin C is not found extracellularly Acremonium chrysogenum
5.1.1.17 biotechnology The results indicate that Penecillium chrysogenum can be used as heterologous host for the production of deacetylcephalosporin C Acremonium chrysogenum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.175 Penicillium chrysogenum strains expressing cefD1, cefD2, cefEF, and cefG genes cloned form Acremonium chrysogenum are constructed, deacetylcephalosporin-C acetyltransferase is coded by the cefG gene Acremonium chrysogenum
5.1.1.17 Penecillium chrysogenum strains are constructed expressing cefD1 (isopenecillin N-CoA synthetase), cefD2 (isopenecillin N-CoA epimerase), cefEF (deacetylcephalosporin-actyltransferase), and cefG (deacetylcephalosporin C synthetase) genes cloned from Acremonium chrysogenum. HPLC analysis of cell extracts show that transformant TA64, TA71, and TA98 accumulate intracellularly deacetylcephalosporin C and, in the last strain (TA98), also cephalosporin C. Acremonium chrysogenum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.175 protoplast
-
Acremonium chrysogenum
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.164 phenylacetyl-CoA + isopenicillin N + H2O Penicillium chrysogenum
-
CoA + penicillin G + L-2-aminohexanedioate
-
?
2.3.1.175 acetyl-CoA + deacetylcephalosporin C Acremonium chrysogenum
-
CoA + cephalosporin C
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.164 Penicillium chrysogenum
-
Penicillium chrysogenum npe6 lacks isopenicillin N acyltransferase activity
-
2.3.1.175 Acremonium chrysogenum
-
-
-
2.3.1.175 Acremonium chrysogenum
-
expression in Penicillium chrysogenum
-
5.1.1.17 Acremonium chrysogenum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.175 6.7
-
deacetylcephalosporin-C acetyltransferase activity in the host strain Penicillium chrysogenum TA64 Acremonium chrysogenum
2.3.1.175 7.2
-
deacetylcephalosporin-C acetyltransferase activity in the host strain Penicillium chrysogenum TA71 Acremonium chrysogenum
2.3.1.175 25.8
-
deacetylcephalosporin-C acetyltransferase activity in the host strain Penicillium chrysogenum TA2 Acremonium chrysogenum
2.3.1.175 30
-
deacetylcephalosporin-C acetyltransferase activity in the host strain Penicillium chrysogenum TA98 Acremonium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.164 phenylacetyl-CoA + isopenicillin N + H2O
-
Penicillium chrysogenum CoA + penicillin G + L-2-aminohexanedioate
-
?
2.3.1.175 acetyl-CoA + deacetylcephalosporin C
-
Acremonium chrysogenum CoA + cephalosporin C
-
?
5.1.1.17 isopenicillin N
-
Acremonium chrysogenum penicillin N
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.164 isopenicillin N acyltransferase
-
Penicillium chrysogenum
2.3.1.164 isopenicillin-N N-acyltransferase
-
Penicillium chrysogenum
2.3.1.175 acetyl-CoA:DAC-acetyltransferase
-
Acremonium chrysogenum
2.3.1.175 deacetylcephalosporin-C acetyltransferase
-
Acremonium chrysogenum
5.1.1.17 isopenecillin N-CoA epimerase
-
Acremonium chrysogenum
5.1.1.17 isopenicillin N epimerase
-
Acremonium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.175 37
-
activity assay Acremonium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.175 7
-
activity assay Acremonium chrysogenum