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Literature summary extracted from
- Differential response of cultured parsley cells to elicitors from two non-pathogenic strains of fungi. Microsomal conversion of (+)marmesin into psoralen (1986), Eur. J. Biochem., 161, 391-398.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.141 | additional information | no effect on enzyme activity by 2-mercaptoethanol and DTT, elicitor fractions isolated from cell walls of Alternaria carthami Chowdhury and of Phythophthora megasperma Drechsler f. sp. glycinea induce the enzyme, the endoplasmic reticulum of cultured parsley cells is the primary target in the differential induction by elicitors from these two non-pathogenic strains of fungi. | Petroselinum crispum | |
| 1.14.14.141 | NADH | NADH acts synergistically with NADPH, but cannot substitute for NADPH | Petroselinum crispum |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.141 | Ancymidole | 48% inhibition of the psoralen synthase at 0.05 mM, 29% at 0.005 mM | Petroselinum crispum | |
| 1.14.14.141 | BAS 110 | 34% inhibition of the psoralen synthase at 0.001 mM, 30% at 0.0002 mM | Petroselinum crispum | |
| 1.14.14.141 | BAS 111 | 52% inhibition of the psoralen synthase at 0.001 mM, 33% at 0.0002 mM | Petroselinum crispum | |
| 1.14.14.141 | BAS 978 | 10% inhibition of the psoralen synthase at 0.001 mM | Petroselinum crispum | |
| 1.14.14.141 | carbon monoxide | - |
Petroselinum crispum | |
| 1.14.14.141 | cytochrome c | 72% inhibition at 0.5 nM | Petroselinum crispum | |
| 1.14.14.141 | ketoconazole | 38% inhibition of the psoralen synthase at 0.050 mM, 20% at 0.005 mM | Petroselinum crispum | |
| 1.14.14.141 | additional information | no effect on enzyme activity by 2-mercaptoethanol and DTT | Petroselinum crispum | |
| 1.14.14.141 | NADP+ | 43% product inhibition at 1 mM | Petroselinum crispum | |
| 1.14.14.141 | Tetcyclacis | 47% inhibition of the psoralen synthase at 0.05 mM, 51% at 0.005 mM | Petroselinum crispum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.141 | 0.052 | - |
NADPH | pH 7.0, 20°C, microsomes | Petroselinum crispum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.141 | endoplasmic reticulum | - |
Petroselinum crispum | 5783 | - |
| 1.14.14.141 | microsome | - |
Petroselinum crispum | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.141 | additional information | Mg2+ does not affect the enzyme activity | Petroselinum crispum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.141 | (+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2 | Petroselinum crispum | - |
psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.141 | Petroselinum crispum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.141 | native enzyme partially by preparation of microsomes | Petroselinum crispum |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.14.141 | (+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2 = psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O | reaction mechanism | Petroselinum crispum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.141 | cell suspension culture | - |
Petroselinum crispum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.141 | (+)-marmesin + NADP + H+ + O2 | - |
Petroselinum crispum | psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + H2O | - |
r | |
| 1.14.14.141 | (+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Petroselinum crispum | psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O | - |
? | |
| 1.14.14.141 | (+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2 | very poor activity with (-)-marmesin, i.e. nodakenetin | Petroselinum crispum | psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O | - |
? | |
| 1.14.14.141 | additional information | the psoralen synthase is a cytochrome-P450-dependent monooxygenase | Petroselinum crispum | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.141 | 20 | - |
assay at | Petroselinum crispum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.141 | 95 | - |
short exposure leads to complete inactivation | Petroselinum crispum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.141 | 7 | 7.25 | - |
Petroselinum crispum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.141 | additional information | the psoralen synthase is a cytochrome-P450-dependent monooxygenase | Petroselinum crispum |
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