EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.12 | for expression in Escherichia coli BL21DE | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.1.12 | C153S | mutant, having only one cysteine in the catalytic site, is oxidized and glutathionylated similarly to the wild-type enzyme | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.12 | CuCl2 | 1 mM | Arabidopsis thaliana | |
1.2.1.12 | Diamide | 1 mM | Arabidopsis thaliana | |
1.2.1.12 | glutathione | 5 mM oxidized glutathione, GSSG, formation of mixed disulfide between glutathione and A4-GAPDH results in the inhibition of enzyme activity | Arabidopsis thaliana | |
1.2.1.12 | glutathione | formation of mixed disulfide between glutathione and GAPDH results in the inhibition of enzyme activity | Spinacia oleracea | |
1.2.1.12 | H2O2 | 1 mM | Arabidopsis thaliana | |
1.2.1.12 | H2O2 | - |
Spinacia oleracea |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.2.1.12 | chloroplast | - |
Spinacia oleracea | 9507 | - |
1.2.1.12 | chloroplast | - |
Arabidopsis thaliana | 9507 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 36140 | - |
theoretical mass of the A subunit | Spinacia oleracea |
1.2.1.12 | 39360 | - |
theoretical mass of the B subunit | Spinacia oleracea |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.12 | 3-phospho-D-glyceroyl phosphate + NADH + H+ | Spinacia oleracea | - |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
? | |
1.2.1.12 | 3-phospho-D-glyceroyl phosphate + NADH + H+ | Arabidopsis thaliana | - |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.12 | Arabidopsis thaliana | - |
- |
- |
1.2.1.12 | Spinacia oleracea | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.2.1.12 | glutathionylation | - |
Arabidopsis thaliana |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.12 | - |
Arabidopsis thaliana |
1.2.1.12 | native GAPDH is purified from spinach chloroplasts | Spinacia oleracea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.12 | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
Spinacia oleracea | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
? | |
1.2.1.12 | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
Arabidopsis thaliana | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.12 | heterotetramer | A2B2-GAPDH, light conformation, B subunits are almost identical to A subunits, expect for the presence of a C-terminal extension containing a pair of cysteines, which is the target of TRX regulation | Spinacia oleracea |
1.2.1.12 | homotetramer | A4-GAPDH | Arabidopsis thaliana |
1.2.1.12 | multimer | A8B8-GAPDH, dark conformation, B subunits are almost identical to A subunits, expect for the presence of a C-terminal extension containing a pair of cysteines, which is the target of TRX regulation | Spinacia oleracea |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.12 | A4-GAPDH | - |
Arabidopsis thaliana |
1.2.1.12 | A4-glyceraldehyde-3-phosphate dehydrogenase | - |
Arabidopsis thaliana |
1.2.1.12 | AnBn-GAPDH | - |
Spinacia oleracea |
1.2.1.12 | GAPDH | - |
Spinacia oleracea |
1.2.1.12 | GAPDH | - |
Arabidopsis thaliana |
1.2.1.12 | glyceraldehyde-3-phosphate dehydrogenase | - |
Spinacia oleracea |
1.2.1.12 | glyceraldehyde-3-phosphate dehydrogenase | - |
Arabidopsis thaliana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 25 | - |
activity assay | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 7.9 | - |
activity assay | Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.12 | NADH | - |
Spinacia oleracea | |
1.2.1.12 | NADH | - |
Arabidopsis thaliana | |
1.2.1.12 | NADPH | - |
Spinacia oleracea |