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Literature summary extracted from

  • Almeida, M.G.; Silveira, C.M.; Guigliarelli, B.; Bertrand, P.; Moura, J.J.; Moura, I.; Leger, C.
    A needle in a haystack: The active site of the membrane-bound complex cytochrome c nitrite reductase (2007), FEBS Lett., 581, 284-288.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.2 CO CO and nitrite both bind to the catalytic heme, bimolecular inhibition kinetics, overview; competitive inhibitor Desulfovibrio desulfuricans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.2 additional information
-
additional information kinetics, overview Desulfovibrio desulfuricans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.2 membrane bound Desulfovibrio desulfuricans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.2 Fe2+ the NrfA2NrfH complex houses 14 hemes Desulfovibrio desulfuricans

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.2 Desulfovibrio desulfuricans
-
strain ATCC 27774
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.2 additional information active site structure determination and analysis, proton transfer is coupled to electron transfer at the active site, overview Desulfovibrio desulfuricans ?
-
?
1.7.2.2 nitrite + reduced methyl viologen
-
Desulfovibrio desulfuricans NH3 + oxidized methyl viologen + H2O
-
?
1.7.2.2 nitrite + reduced methyl viologen nitrite and inhibitor CO both bind to the catalytic heme Desulfovibrio desulfuricans NH3 + H2O + oxidized methyl viologen
-
?

Synonyms

EC Number Synonyms Comment Organism
1.7.2.2 ccNiR
-
Desulfovibrio desulfuricans
1.7.2.2 cytochrome c nitrite reductase
-
Desulfovibrio desulfuricans
1.7.2.2 NrfA2NrfH complex
-
Desulfovibrio desulfuricans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.2.2 20
-
assay at Desulfovibrio desulfuricans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.2 340
-
nitrite at pH 7.6, 20°C, in phosphate buffer Desulfovibrio desulfuricans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.2 7.6
-
assay at Desulfovibrio desulfuricans

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.2 heme contains 14 hemes Desulfovibrio desulfuricans
1.7.2.2 heme the NrfA2NrfH complex houses 14 hemes, closely-spaced hemes in this enzyme with magnetic interactions between them Desulfovibrio desulfuricans
1.7.2.2 methyl viologen artificial cofactor Desulfovibrio desulfuricans