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Literature summary extracted from

  • Alvarez, V.; Rodriguez-Saiz, M.; de la Fuente, J.L.; Gudina, E.J.; Godio, R.P.; Martin, J.F.; Barredo, J.L.
    The crtS gene of Xanthophyllomyces dendrorhous encodes a novel cytochrome-P450 hydroxylase involved in the conversion of beta-carotene into astaxanthin and other xanthophylls (2006), Fungal Genet. Biol., 43, 261-272.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.1 gene crtS, DNA and amino acid sequence determination of different strains Phaffia rhodozyma
1.14.15.24 expressed in Mucor circinelloides strain MS12 Phaffia rhodozyma

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.1 additional information construction and analysis of insertion mutant strains defective in biosynthesis of astaxanthin, the mutants are selected by accumulation of astaxanthin precursors Phaffia rhodozyma

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.1 Fe2+ heme iron Phaffia rhodozyma

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.24 62600
-
 deduced from amino acid sequence Phaffia rhodozyma

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.1 additional information Phaffia rhodozyma the enzyme is involved in the biosynthesis of astaxanthin, overview ?
-
 ?
1.14.14.1 additional information Phaffia rhodozyma VKPM Y2410 the enzyme is involved in the biosynthesis of astaxanthin, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.1 Phaffia rhodozyma
-
 wild-type strains ATCC 24203, ATCC 96594, and ATCC 96815, and astaxanthin-overproducing recombinant strains VKPM and Y2410, gene crtS
-
1.14.15.24 Phaffia rhodozyma
-
-
-
1.14.15.24 Phaffia rhodozyma VKPM Y2410
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.1 additional information the enzyme is involved in the biosynthesis of astaxanthin, overview Phaffia rhodozyma ?
-
 ?
1.14.14.1 additional information the enzyme is involved in the biosynthesis of astaxanthin, overview Phaffia rhodozyma VKPM Y2410 ?
-
 ?
1.14.15.24 3'-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Phaffia rhodozyma adonixanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 3'-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Phaffia rhodozyma VKPM Y2410 adonixanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 3-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Phaffia rhodozyma 4-ketozeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 3-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Phaffia rhodozyma VKPM Y2410 4-ketozeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Phaffia rhodozyma beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O beta-cryptoxanthin is 3-hydroxy-beta,beta-carotene ?
1.14.15.24 beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Phaffia rhodozyma VKPM Y2410 beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O beta-cryptoxanthin is 3-hydroxy-beta,beta-carotene ?
1.14.15.24 beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
 Phaffia rhodozyma zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O zeaxanthin is 3,3'-dihydroxy-beta,beta-carotene ?
1.14.15.24 beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
 Phaffia rhodozyma VKPM Y2410 zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O zeaxanthin is 3,3'-dihydroxy-beta,beta-carotene ?
1.14.15.24 beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Phaffia rhodozyma zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 canthaxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 canthaxanthin is beta,beta-carotene-4-4'-dione Phaffia rhodozyma phoenicoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 echinenone + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 echinenone is beta,beta-carotene-4-one Phaffia rhodozyma 3-hydroxyechinenone + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 phoenicoxanthin + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
 Phaffia rhodozyma astaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O astaxanthin is 3,3'-dihydroxy-beta,beta-carotene-4-4'-dione ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.1 cytochrome-P450 hydroxylase
-
 Phaffia rhodozyma
1.14.15.24 crtS
-
 Phaffia rhodozyma

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.15.24 Phaffia rhodozyma deduced from amino acid sequence
-
 5.8