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Literature summary extracted from

  • Johnson, E.F.; Mukhopadhyay, B.
    A new type of sulfite reductase, a novel coenzyme F420-dependent enzyme, from the methanarchaeon Methanocaldococcus jannaschii (2005), J. Biol. Chem., 280, 38776-38786.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.2 coenzyme F420
-
Methanocaldococcus jannaschii
1.8.7.1 coenzyme F420
-
Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.2 0.0122
-
sulfite within a sulfite concentration range of 0.0014-0.3 mM and with a fixed reduced F420 concentration of 0.04 mM Methanocaldococcus jannaschii
1.8.1.2 0.021
-
reduced F420 at a fixed sulfite concentration of 0.29 mM and within a reduced F420 concentration range of 0.004-0.06 mM Methanocaldococcus jannaschii
1.8.7.1 0.0122
-
sulfite within a sulfite concentration range of 0.0014-0.3 mM and with a fixed reduced coenzyme F420 concentration of 0.04 mM Methanocaldococcus jannaschii
1.8.7.1 0.021
-
reduced coenzyme F420 at a fixed sulfite concentration of 0.29 mM and within a reduced coenzyme F420 concentration range of 0.004-0.06 mM Methanocaldococcus jannaschii
1.8.98.3 0.012
-
sulfite pH 7.0, temperature not specified in the publication Methanocaldococcus jannaschii
1.8.98.3 0.021
-
reduced coenzyme F420 pH 7.0, temperature not specified in the publication Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.2 70000
-
SDS-PAGE Methanocaldococcus jannaschii
1.8.1.2 350000
-
gel filtration Methanocaldococcus jannaschii
1.8.7.1 70000
-
SDS-PAGE Methanocaldococcus jannaschii
1.8.7.1 350000
-
gel filtration Methanocaldococcus jannaschii
1.8.98.3 70000
-
x * 70000, SDS-PAGE Methanocaldococcus jannaschii
1.8.98.3 350000
-
gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.98.3 sulfite + reduced coenzyme F420 Methanocaldococcus jannaschii sulfite detoxification and assimilation mechanisms hydrogen sulfide + oxidized coenzyme F420
-
?
1.8.98.3 sulfite + reduced coenzyme F420 Methanocaldococcus jannaschii DSM 2661 sulfite detoxification and assimilation mechanisms hydrogen sulfide + oxidized coenzyme F420
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.2 Methanocaldococcus jannaschii
-
-
-
1.8.7.1 Methanocaldococcus jannaschii
-
-
-
1.8.98.3 Methanocaldococcus jannaschii Q58280
-
-
1.8.98.3 Methanocaldococcus jannaschii DSM 2661 Q58280
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.8.98.3 activity in cell extracts is highly oxygen sensitive. About 92% activity is lost if the cell extract is exposed to air for 60 min. Dithiothreitol and 2-mercapotoethanol neither protect the enzyme from inactivation nor restore activity in an air-inactivated enzyme preparation Methanocaldococcus jannaschii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.2 phenyl-Sepharose chromatography, F420-Sepharose chromatography and QAE-Sephadex gel filtration Methanocaldococcus jannaschii
1.8.7.1 phenyl-Sepharose chromatography, F420-Sepharose chromatography and QAE-Sephadex gel filtration Methanocaldococcus jannaschii
1.8.98.3
-
Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.2 0.0013
-
in 50 mM potassium phosphate buffer (pH 7), 0.044 mM reduced F420, and 1.5 mM sodium sulfite, from cell extract Methanocaldococcus jannaschii
1.8.1.2 0.0182
-
in 50 mM potassium phosphate buffer (pH 7), 0.044 mM reduced F420, and 1.5 mM sodium sulfite, after 14fold purification Methanocaldococcus jannaschii
1.8.7.1 0.0013
-
in 50 mM potassium phosphate buffer (pH 7), 0.044 mM reduced coenzyme F420, and 1.5 mM sodium sulfite, from cell extract Methanocaldococcus jannaschii
1.8.7.1 0.0182
-
in 50 mM potassium phosphate buffer (pH 7), 0.044 mM reduced coenzyme F420, and 1.5 mM sodium sulfite, after 14fold purification Methanocaldococcus jannaschii
1.8.98.3 0.09
-
pH 7.0, temperature not specified in the publication, substrate: sulfite and reduced methyl viologen Methanocaldococcus jannaschii
1.8.98.3 1.3
-
pH 7.0, temperature not specified in the publication, cell extract, substrate: sulfite and reduced coenzyme F420 Methanocaldococcus jannaschii
1.8.98.3 18.2
-
pH 7.0, temperature not specified in the publication, purified enzyme, substrate: sulfite and reduced coenzyme F420 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.2 sulfite + reduced F420
-
Methanocaldococcus jannaschii sulfide + oxidized F420
-
?
1.8.7.1 sulfite + reduced coenzyme F420
-
Methanocaldococcus jannaschii sulfide + oxidized coenzyme F420
-
?
1.8.98.3 sulfite + reduced coenzyme F420 sulfite detoxification and assimilation mechanisms Methanocaldococcus jannaschii hydrogen sulfide + oxidized coenzyme F420
-
?
1.8.98.3 sulfite + reduced coenzyme F420 no activity with: thiosulfate, sulfate, NADH and NADPH Methanocaldococcus jannaschii hydrogen sulfide + oxidized coenzyme F420
-
?
1.8.98.3 sulfite + reduced coenzyme F420 sulfite detoxification and assimilation mechanisms Methanocaldococcus jannaschii DSM 2661 hydrogen sulfide + oxidized coenzyme F420
-
?
1.8.98.3 sulfite + reduced coenzyme F420 no activity with: thiosulfate, sulfate, NADH and NADPH Methanocaldococcus jannaschii DSM 2661 hydrogen sulfide + oxidized coenzyme F420
-
?
1.8.98.3 sulfite + reduced methyl viologen no activity with: thiosulfate, sulfate, NADH and NADPH Methanocaldococcus jannaschii hydrogen sulfide + oxidized methyl viologen
-
?
1.8.98.3 sulfite + reduced methyl viologen no activity with: thiosulfate, sulfate, NADH and NADPH Methanocaldococcus jannaschii DSM 2661 hydrogen sulfide + oxidized methyl viologen
-
?

Subunits

EC Number Subunits Comment Organism
1.8.98.3 ? x * 70000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
1.8.1.2 coenzyme F420-dependent sulfite reductase
-
Methanocaldococcus jannaschii
1.8.1.2 Fsr
-
Methanocaldococcus jannaschii
1.8.1.2 sulfite reductase
-
Methanocaldococcus jannaschii
1.8.7.1 coenzyme F420-dependent sulfite reductase
-
Methanocaldococcus jannaschii
1.8.7.1 Fsr
-
Methanocaldococcus jannaschii
1.8.7.1 sulfite reductase
-
Methanocaldococcus jannaschii
1.8.98.3 coenzyme F420-dependent sulfite reductase
-
Methanocaldococcus jannaschii
1.8.98.3 Fsr
-
Methanocaldococcus jannaschii
1.8.98.3 MJ0870 locus name Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.2 95
-
-
Methanocaldococcus jannaschii
1.8.7.1 95
-
-
Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.2 7
-
-
Methanocaldococcus jannaschii
1.8.7.1 7
-
-
Methanocaldococcus jannaschii
1.8.98.3 7
-
assay at Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.98.3 siroheme the N-terminal half of the 70-kDa Fsr polypeptide represents a H2F420 dehydrogenase and the C-terminal half a dissimilatory-type siroheme sulfite reductase Methanocaldococcus jannaschii

Expression

EC Number Organism Comment Expression
1.8.98.3 Methanocaldococcus jannaschii sulfite induces coenzyme F420-dependent sulfite reductase up

General Information

EC Number General Information Comment Organism
1.8.98.3 physiological function sulfite detoxification and assimilation mechanisms Methanocaldococcus jannaschii