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Literature summary extracted from

  • Kavanagh, K.L.; Dunford, J.E.; Bunkoczi, G.; Russell, R.G.; Oppermann, U.
    The crystal structure of human geranylgeranyl pyrophosphate synthase reveals a novel hexameric arrangement and inhibitory product binding (2006), J. Biol. Chem., 281, 22004-22012.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.29 Tween 20 at 0.2% Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.29 expression of N-terminally His-tagged enzyme in Escherichia coli Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.29 native protein, 20°C, sitting drop vapour diffusion mthod, mixing of 200 nl of 90 mg/ml protein in 10 mM HEPES, pH 7.5, 500 mM NaCl, 5% glycerol with 100 nl of precipitant solution consisting of 25% PEG 3350, 200 mM magnesium formate, pH 5.5, and equilibration against 0.1 ml of the precipitant solution, selenomethionine-labeled protein by suspending a 0.003 ml drop containing 26 mg/ml protein, 333 mM NaCl, 0.67 mM MgCl2, 0.67 mM GGPP, 3% glycerol, 15% 2-methyl-2,4-pentanediol, 1.7% PEG 10,000, 6.7 mM HEPES, pH 7.5, over a 1 ml reservoir containing 45% 2-methyl-2,4-pentanediol, and 5% PEG 10000, X-ray diffraction structure determination and analysis at 2.7-2.8 A resolution Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.29 EDTA
-
 Homo sapiens
2.5.1.29 geranylgeranyl diphosphate competitive product inhibition Homo sapiens
2.5.1.29 nitrogen-containing bisphosphonates
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.29 additional information
-
 additional information steady-state kinetics, recombinant enzyme Homo sapiens
2.5.1.29 0.003
-
 isopentenyl diphosphate pH 7.7, 37°C, recombinant enzyme Homo sapiens
2.5.1.29 0.0042
-
 trans,trans-farnesyl diphosphate pH 7.7, 37°C, recombinant enzyme Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.29 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.29 200000
-
 about, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.29 trans,trans-farnesyl diphosphate + isopentenyl diphosphate Homo sapiens the enzyme takes part in the mevalonate pathway of isoprenoid synthesis, and plays a crucial role in cell survival, overview diphosphate + geranylgeranyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.29 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.29 recombinant N-terminally His-tagged enzyme from Escherichia coli, the His-tag is cleaved of by TEV Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.29 trans,trans-farnesyl diphosphate + isopentenyl diphosphate the enzyme takes part in the mevalonate pathway of isoprenoid synthesis, and plays a crucial role in cell survival, overview Homo sapiens diphosphate + geranylgeranyl diphosphate
-
 ?
2.5.1.29 trans,trans-farnesyl diphosphate + isopentenyl diphosphate tight binding substrate and product structures, overview Homo sapiens diphosphate + geranylgeranyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.29 hexamer three dimers join together to form a propeller-bladed hexameric molecule, quaternary structure, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.5.1.29 geranylgeranyl pyrophosphate synthase
-
 Homo sapiens
2.5.1.29 GGPS
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.29 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.29 0.204
-
 trans,trans-farnesyl diphosphate pH 7.7, 37°C, recombinant enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.29 7.7
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.29 0.025
-
 geranylgeranyl diphosphate pH 7.7, 37°C, recombinant enzyme, versus trans,trans-farnesyl diphosphate Homo sapiens