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Literature summary extracted from

  • Unno, H.; Uchida, T.; Sugawara, H.; Kurisu, G.; Sugiyama, T.; Yamaya, T.; Sakakibara, H.; Hase, T.; Kusunoki, M.
    Atomic structure of plant glutamine synthetase: a key enzyme for plant productivity (2006), J. Biol. Chem., 281, 29287-29296.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.2 expressed in Escherichia coli JM109 cells Zea mays

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.1.2 hanging drop vapour diffusion method, GS crystals in complex with ADP and methionine sulfoximine phosphate, using 9% (w/v) polyethylene glycol 8000, 100 mM Tris-HCl pH 7.8, 5% (v/v) 2-methyl-2,4-pentanediol, 10 mM MnCl2 Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Mn2+ essential for activity, contains three Mn2+ ions per subunit Zea mays

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2
-
 Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
 Zea mays ADP + phosphate + L-Gln
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 decamer x-ray crystallography Zea mays

Synonyms

EC Number Synonyms Comment Organism
6.3.1.2 Glutamine synthetase
-
 Zea mays
6.3.1.2 GS1a
-
 Zea mays