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Literature summary extracted from

  • Luka, Z.; Pakhomova, S.; Loukachevitch, L.V.; Egli, M.; Newcomer, M.E.; Wagner, C.
    5-methyltetrahydrofolate is bound in intersubunit areas of rat liver folate-binding protein glycine N-methyltransferase (2007), J. Biol. Chem., 282, 4069-4075.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.20 expressed in Escherichia coli BL21(DE3) Rattus norvegicus
2.1.1.20 expressed in Escherichia coli BL21(GE3) Rattus norvegicus
2.1.1.20 into pET-17b vector and expressed in Escherichia coli BL21(GE3) Rattus norvegicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.20 crystallized by the sitting drop method in complex with (6S)-5-methyltetrahydrofolat monoglutamate Rattus norvegicus
2.1.1.20 GNMT complexed with 5-methyltetrahydrofolate, by the sitting drop method at room temperature, two folate binding sites in the intersubunit areas of the tetramer, each folate binding site is formed primarily by two 1-7 N-terminal regions of one pair of subunits and two 205-218 regions of the other pair of subunits. Both the pteridine and p-aminobenzoyl rings are located in the hydrophobic cavities formed by Tyr5, Leu207, and Met215 residues of all subunits Rattus norvegicus
2.1.1.20 sitting-drop vapor diffusion method in complex with 5-methyltetrahydrofolate pentaglutamate, two molecules of inhibitor bound to a tetramer Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.20 5-methyltetrahydrofolate
-
Rattus norvegicus
2.1.1.20 5-Methyltetrahydrofolate pentaglutamate
-
Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.20 cytoplasm
-
Rattus norvegicus 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.20 additional information Rattus norvegicus major folate binding protein ?
-
?
2.1.1.20 S-adenosyl-L-methionine + glycine Rattus norvegicus key regulatory enzyme for methyl group metabolism by regulating the S-adenosyl-L-methionine/S-adenosyl-L-homocysteine ratio S-adenosyl-L-homocysteine + N-methylglycine
-
?
2.1.1.20 S-adenosyl-L-methionine + glycine Rattus norvegicus key enzyme for the regulation of the ratio of S-adenosylmethionine to S-adenosylhomocysteine, control of the methylating potential of the cell S-adenosyl-L-homocysteine + N-methylglycine N-methylglycine = sarcosine ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.20 Rattus norvegicus P13255
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.20 by ammonium sulfate precipitation and gel filtration Rattus norvegicus
2.1.1.20 recombinant protein Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.20 liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.20 additional information major folate binding protein Rattus norvegicus ?
-
?
2.1.1.20 S-adenosyl-L-methionine + glycine key regulatory enzyme for methyl group metabolism by regulating the S-adenosyl-L-methionine/S-adenosyl-L-homocysteine ratio Rattus norvegicus S-adenosyl-L-homocysteine + N-methylglycine
-
?
2.1.1.20 S-adenosyl-L-methionine + glycine key enzyme for the regulation of the ratio of S-adenosylmethionine to S-adenosylhomocysteine, control of the methylating potential of the cell Rattus norvegicus S-adenosyl-L-homocysteine + N-methylglycine N-methylglycine = sarcosine ?

Subunits

EC Number Subunits Comment Organism
2.1.1.20 tetramer crystal structure analysis Rattus norvegicus
2.1.1.20 tetramer crystallographic studies Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.20 GNMT
-
Rattus norvegicus