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Literature summary extracted from

  • Kalra, S.; Paul, M.K.; Balaram, H.; Mukhopadhyay, A.K.
    Application of HPLC to study the kinetics of a branched bi-enzyme system consisting of hypoxanthine-guanine phosphoribosyltransferase and xanthine oxidase - an important biochemical system to evaluate the efficiency of the anticancer drug 6-mercaptopurine (2007), J. Chromatogr. B, 850, 7-14.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.8 analysis the branched bi-enzyme system with xanthine oxidase is an important biochemical system to evaluate the efficiency of the anticancer drug 6-mercaptopurine, overview Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.8 expressed in Escherichia coli strain BL21(DE3) Homo sapiens
2.4.2.8 gene HGPRT, expression in Escherichia coli strain BL21(DE3) Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 additional information
-
 additional information kinetic analysis of the branched bi-enzyme system, detailed overview Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.3.2 extracellular
-
 Homo sapiens
-
-
2.4.2.8 cytoplasm
-
 Homo sapiens 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.8 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.3.2 6-mercaptopurine + 2 H2O + 2 O2 Bos taurus
-
 6-thiouric acid + 2 H2O2
-
 ?
1.17.3.2 6-mercaptopurine + 2 H2O + 2 O2 Homo sapiens an anticancer prodrug, no activation by xanthine oxidase but conversion to the inactive metabolite 6-thiouric acid, catabolism, overview 6-thiouric acid + 2 H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2 Homo sapiens
-
 urate + H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2 Bos taurus
-
 urate + H2O2
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Homo sapiens
-
 IMP + diphosphate
-
 ?
2.4.2.8 additional information Homo sapiens the enzyme is organized in a branched bi-enzyme system with xanthine oxidase, EC 1.17.3.2 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.3.2 Bos taurus
-
-
-
1.17.3.2 Homo sapiens
-
-
-
2.4.2.8 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.3.2 commercial preparation
-
 Bos taurus
-
1.17.3.2 JURKAT cell
-
 Homo sapiens
-
1.17.3.2 lymphocyte
-
 Homo sapiens
-
1.17.3.2 milk
-
 Bos taurus
-
1.17.3.2 plasma
-
 Homo sapiens
-
2.4.2.8 acute lymphoblastic leukemia cell
-
 Homo sapiens
-
2.4.2.8 JURKAT cell
-
 Homo sapiens
-
2.4.2.8 JURKAT cell a T-ALL cell line Homo sapiens
-
2.4.2.8 lymphocyte immature Homo sapiens
-
2.4.2.8 T-ALL cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.3.2 additional information
-
 rate of extracellular catabolism of 6-mercaptopurine by XOD Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.3.2 6-mercaptopurine + 2 H2O + 2 O2
-
 Homo sapiens 6-thiouric acid + 2 H2O2
-
 ?
1.17.3.2 6-mercaptopurine + 2 H2O + 2 O2
-
 Bos taurus 6-thiouric acid + 2 H2O2
-
 ?
1.17.3.2 6-mercaptopurine + 2 H2O + 2 O2 an anticancer prodrug, no activation by xanthine oxidase but conversion to the inactive metabolite 6-thiouric acid, catabolism, overview Homo sapiens 6-thiouric acid + 2 H2O2
-
 ?
1.17.3.2 additional information evaluation of a HPLC detection method for enzyme reaction products, overview Homo sapiens ?
-
 ?
1.17.3.2 additional information evaluation of a HPLC detection method for enzyme reaction products, overview Bos taurus ?
-
 ?
1.17.3.2 xanthine + H2O + O2
-
 Homo sapiens urate + H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2
-
 Bos taurus urate + H2O2
-
 ?
2.4.2.8 6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate a thiopurine antimetabolite Homo sapiens thioinosinic monophosphate + diphosphate a therapeutically active metabolite ?
2.4.2.8 6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens 6-thioinosine monophosphate + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens IMP + diphosphate
-
 ?
2.4.2.8 additional information the enzyme is organized in a branched bi-enzyme system with xanthine oxidase, EC 1.17.3.2 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.3.2 XOD
-
 Homo sapiens
1.17.3.2 XOD
-
 Bos taurus
2.4.2.8 HGPRT
-
 Homo sapiens
2.4.2.8 hypoxanthine-guanine phosphoribosyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.3.2 37
-
 assay at Homo sapiens
1.17.3.2 37
-
 assay at Bos taurus
2.4.2.8 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.3.2 7.4
-
 assay at Homo sapiens
1.17.3.2 7.4
-
 assay at Bos taurus
2.4.2.8 7.4
-
 assay at Homo sapiens