BRENDA - Enzyme Database

Phosphatidylinositol synthase of Tetrahymena: inositol isomers as substrates in phosphatidylinositol biosynthesis and headgroup exchange reactions

Riggs, B.M.; Lansley, T.A.; Ryals, P.E.; J. Eukaryot. Microbiol. 54, 119-124 (2007)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.11
microsome
; microsomal fractions from Tetrahymena vorax, positive for NADPH-cytochrome c reductase activity
Tetrahymena vorax
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.11
Mg2+
less effect at more than 5 mM; stimulation, maximum catalytic activity in presence of 2 mM MgCl2 plus 2 mM MnCl2. Less stimulatory above 5 mM
Tetrahymena vorax
2.7.8.11
Mn2+
greater effect than Mg2+, less effect at more than 5 mM; stimulation, maximum catalytic activity in presence of 2 mM MgCl2 plus 2 mM MnCl2. Less stimulatory above 5 mM
Tetrahymena vorax
2.7.8.11
additional information
slight or no effect by calcium, cobalt, copper, iron, lithium, molybdenum, zinc ions; synergistic effect of Mg2+ and Mn2+, optimum condition: 2 mM MgCl2, 2 mM MnCl2
Tetrahymena vorax
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.11
Tetrahymena vorax
-
-
-
2.7.8.11
Tetrahymena vorax V2S
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.11
CDP-diacylglycerol + 1D-chiro-inositol
conversion of 30.9% CDP-diacylglycerol (utilization significant compared to background, p: 0.05), microsomal fraction, 30C, 20 min
675120
Tetrahymena vorax
CMP + phosphatidyl-1D-chiro-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + 1L-chiro-inositol
conversion of 25.1% CDP-diacylglycerol (non-significant), microsomal fraction, 30C, 20 min
675120
Tetrahymena vorax
CMP + phosphatidyl-1L-chiro-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + allo-inositol
83% of the activity on myo-inositol
675120
Tetrahymena vorax
CMP + phosphatidyl-allo-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + allo-inositol
conversion of 23.2% CDP-diacylglycerol (non-significant), microsomal fraction, 30C, 20 min
675120
Tetrahymena vorax
CMP + phosphatidyl-allo-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + allo-inositol
83% of the activity on myo-inositol
675120
Tetrahymena vorax V2S
CMP + phosphatidyl-allo-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + allo-inositol
conversion of 23.2% CDP-diacylglycerol (non-significant), microsomal fraction, 30C, 20 min
675120
Tetrahymena vorax V2S
CMP + phosphatidyl-allo-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + D-chiro-inositol
82% of the activity on myo-inositol
675120
Tetrahymena vorax
CMP + phosphatidyl-chiro-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + epi-inositol
62% of the activity on myo-inositol
675120
Tetrahymena vorax
CMP + phosphatidyl-epi-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + epi-inositol
conversion of 31.1% CDP-diacylglycerol (utilization significant compared to background, p: 0.01), microsomal fraction, 30C, 20 min
675120
Tetrahymena vorax
CMP + phosphatidyl-epi-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + L-chiro-inositol
67% of the activity on myo-inositol
675120
Tetrahymena vorax
CMP + phosphatidyl-chiro-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + muco-inositol
41% of the activity on myo-inositol
675120
Tetrahymena vorax
CMP + phosphatidyl-muco-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + muco-inositol
conversion of 15.2% CDP-diacylglycerol (non-significant), microsomal fraction, 30C, 20 min
675120
Tetrahymena vorax
CMP + phosphatidyl-muco-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + myo-inositol
myo-inositol and scyllo-inositol are preferred substrates
675120
Tetrahymena vorax
CMP + phosphatidyl-myo-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + myo-inositol
conversion of 37.4% CDP-diacylglycerol (utilization significant compared to background, p: 0.01), microsomal fraction, standard reaction: 20 min, 30C, pH 8, 2 mM MgCl2, 2 mM MnCl2, 1.6 mM Triton X-100
675120
Tetrahymena vorax
CMP + phosphatidyl-myo-inositol
analyses by liquid scintillation counting and thin-layer chromatography
-
-
?
2.7.8.11
CDP-diacylglycerol + scyllo-inositol
myo-inositol and scyllo-inositol are preferred substrates
675120
Tetrahymena vorax
CMP + phosphatidyl-scyllo-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + scyllo-inositol
conversion of 33.7% CDP-diacylglycerol (utilization significant compared to background, p: 0.01), microsomal fraction, 30C, 20 min
675120
Tetrahymena vorax
CMP + phosphatidyl-scyllo-inositol
-
-
-
?
2.7.8.11
additional information
formation of novel non-myo-inositol-containing second messengers, preferred non-myo-inositols are scyllo- and chiro-inositol
675120
Tetrahymena vorax
?
-
-
-
?
2.7.8.11
additional information
microsomal fraction contains additional PdtIn: inositol phosphatidyl transferase activity (inositol headgroup exchange, 20 min, 30C) between myo-, scyllo-, 1D-chiro-inositol and phosphatidyl-myo-inositol, increased in presence of CMP, least specificity for 1L-chiro- and muco-inositol
675120
Tetrahymena vorax
?
-
-
-
-
2.7.8.11
additional information
myo- and scyllo-inositol preferred inositol isomers for PtdIn synthase activity, least specificity for muco-inositol
675120
Tetrahymena vorax
?
-
-
-
-
2.7.8.11
additional information
formation of novel non-myo-inositol-containing second messengers, preferred non-myo-inositols are scyllo- and chiro-inositol
675120
Tetrahymena vorax V2S
?
-
-
-
?
2.7.8.11
additional information
microsomal fraction contains additional PdtIn: inositol phosphatidyl transferase activity (inositol headgroup exchange, 20 min, 30C) between myo-, scyllo-, 1D-chiro-inositol and phosphatidyl-myo-inositol, increased in presence of CMP, least specificity for 1L-chiro- and muco-inositol
675120
Tetrahymena vorax V2S
?
-
-
-
-
2.7.8.11
additional information
myo- and scyllo-inositol preferred inositol isomers for PtdIn synthase activity, least specificity for muco-inositol
675120
Tetrahymena vorax V2S
?
-
-
-
-
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.8.11
30
-
; pH 7.8
Tetrahymena vorax
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
2.7.8.11
50
-
89% of maximum activity, pH 7.8, 10 min preincubation
Tetrahymena vorax
2.7.8.11
60
-
82% of maximum activity, pH 7.8, 10 min preincubation
Tetrahymena vorax
2.7.8.11
70
-
79% of maximum activity, pH 7.8, 10 min preincubation
Tetrahymena vorax
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.7.8.11
70
-
10 min, 80% residual activity
Tetrahymena vorax
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.11
7.8
-
; 30C
Tetrahymena vorax
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.8.11
7.3
8.4
30C, 90% of maximum activity; about 90% of maximum activity within this range
Tetrahymena vorax
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.11
microsome
; microsomal fractions from Tetrahymena vorax, positive for NADPH-cytochrome c reductase activity
Tetrahymena vorax
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.11
Mg2+
less effect at more than 5 mM; stimulation, maximum catalytic activity in presence of 2 mM MgCl2 plus 2 mM MnCl2. Less stimulatory above 5 mM
Tetrahymena vorax
2.7.8.11
Mn2+
greater effect than Mg2+, less effect at more than 5 mM; stimulation, maximum catalytic activity in presence of 2 mM MgCl2 plus 2 mM MnCl2. Less stimulatory above 5 mM
Tetrahymena vorax
2.7.8.11
additional information
slight or no effect by calcium, cobalt, copper, iron, lithium, molybdenum, zinc ions; synergistic effect of Mg2+ and Mn2+, optimum condition: 2 mM MgCl2, 2 mM MnCl2
Tetrahymena vorax
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.11
CDP-diacylglycerol + 1D-chiro-inositol
conversion of 30.9% CDP-diacylglycerol (utilization significant compared to background, p: 0.05), microsomal fraction, 30C, 20 min
675120
Tetrahymena vorax
CMP + phosphatidyl-1D-chiro-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + 1L-chiro-inositol
conversion of 25.1% CDP-diacylglycerol (non-significant), microsomal fraction, 30C, 20 min
675120
Tetrahymena vorax
CMP + phosphatidyl-1L-chiro-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + allo-inositol
83% of the activity on myo-inositol
675120
Tetrahymena vorax
CMP + phosphatidyl-allo-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + allo-inositol
conversion of 23.2% CDP-diacylglycerol (non-significant), microsomal fraction, 30C, 20 min
675120
Tetrahymena vorax
CMP + phosphatidyl-allo-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + allo-inositol
83% of the activity on myo-inositol
675120
Tetrahymena vorax V2S
CMP + phosphatidyl-allo-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + allo-inositol
conversion of 23.2% CDP-diacylglycerol (non-significant), microsomal fraction, 30C, 20 min
675120
Tetrahymena vorax V2S
CMP + phosphatidyl-allo-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + D-chiro-inositol
82% of the activity on myo-inositol
675120
Tetrahymena vorax
CMP + phosphatidyl-chiro-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + epi-inositol
62% of the activity on myo-inositol
675120
Tetrahymena vorax
CMP + phosphatidyl-epi-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + epi-inositol
conversion of 31.1% CDP-diacylglycerol (utilization significant compared to background, p: 0.01), microsomal fraction, 30C, 20 min
675120
Tetrahymena vorax
CMP + phosphatidyl-epi-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + L-chiro-inositol
67% of the activity on myo-inositol
675120
Tetrahymena vorax
CMP + phosphatidyl-chiro-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + muco-inositol
41% of the activity on myo-inositol
675120
Tetrahymena vorax
CMP + phosphatidyl-muco-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + muco-inositol
conversion of 15.2% CDP-diacylglycerol (non-significant), microsomal fraction, 30C, 20 min
675120
Tetrahymena vorax
CMP + phosphatidyl-muco-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + myo-inositol
myo-inositol and scyllo-inositol are preferred substrates
675120
Tetrahymena vorax
CMP + phosphatidyl-myo-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + myo-inositol
conversion of 37.4% CDP-diacylglycerol (utilization significant compared to background, p: 0.01), microsomal fraction, standard reaction: 20 min, 30C, pH 8, 2 mM MgCl2, 2 mM MnCl2, 1.6 mM Triton X-100
675120
Tetrahymena vorax
CMP + phosphatidyl-myo-inositol
analyses by liquid scintillation counting and thin-layer chromatography
-
-
?
2.7.8.11
CDP-diacylglycerol + scyllo-inositol
myo-inositol and scyllo-inositol are preferred substrates
675120
Tetrahymena vorax
CMP + phosphatidyl-scyllo-inositol
-
-
-
?
2.7.8.11
CDP-diacylglycerol + scyllo-inositol
conversion of 33.7% CDP-diacylglycerol (utilization significant compared to background, p: 0.01), microsomal fraction, 30C, 20 min
675120
Tetrahymena vorax
CMP + phosphatidyl-scyllo-inositol
-
-
-
?
2.7.8.11
additional information
formation of novel non-myo-inositol-containing second messengers, preferred non-myo-inositols are scyllo- and chiro-inositol
675120
Tetrahymena vorax
?
-
-
-
?
2.7.8.11
additional information
microsomal fraction contains additional PdtIn: inositol phosphatidyl transferase activity (inositol headgroup exchange, 20 min, 30C) between myo-, scyllo-, 1D-chiro-inositol and phosphatidyl-myo-inositol, increased in presence of CMP, least specificity for 1L-chiro- and muco-inositol
675120
Tetrahymena vorax
?
-
-
-
-
2.7.8.11
additional information
myo- and scyllo-inositol preferred inositol isomers for PtdIn synthase activity, least specificity for muco-inositol
675120
Tetrahymena vorax
?
-
-
-
-
2.7.8.11
additional information
formation of novel non-myo-inositol-containing second messengers, preferred non-myo-inositols are scyllo- and chiro-inositol
675120
Tetrahymena vorax V2S
?
-
-
-
?
2.7.8.11
additional information
microsomal fraction contains additional PdtIn: inositol phosphatidyl transferase activity (inositol headgroup exchange, 20 min, 30C) between myo-, scyllo-, 1D-chiro-inositol and phosphatidyl-myo-inositol, increased in presence of CMP, least specificity for 1L-chiro- and muco-inositol
675120
Tetrahymena vorax V2S
?
-
-
-
-
2.7.8.11
additional information
myo- and scyllo-inositol preferred inositol isomers for PtdIn synthase activity, least specificity for muco-inositol
675120
Tetrahymena vorax V2S
?
-
-
-
-
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.8.11
30
-
; pH 7.8
Tetrahymena vorax
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
2.7.8.11
50
-
89% of maximum activity, pH 7.8, 10 min preincubation
Tetrahymena vorax
2.7.8.11
60
-
82% of maximum activity, pH 7.8, 10 min preincubation
Tetrahymena vorax
2.7.8.11
70
-
79% of maximum activity, pH 7.8, 10 min preincubation
Tetrahymena vorax
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.7.8.11
70
-
10 min, 80% residual activity
Tetrahymena vorax
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.11
7.8
-
; 30C
Tetrahymena vorax
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.8.11
7.3
8.4
30C, 90% of maximum activity; about 90% of maximum activity within this range
Tetrahymena vorax