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Literature summary extracted from

  • Lim, J.; Lee, J.; Lim, J.; Min, K.R.; Kim, C.; Kim, Y.
    Characterization of the pcbD gene encoding 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase from Pseudomonas sp. P20 (2000), J. Microbiol. Biotechnol., 10, 258-263.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.7.1.8 molecular biology open reading frame corresponding to the pcbD gene consists of 855 base pairs with an ATG initiation codon and a TGA termination codon, able to encode a polypeptide with a molecular weight of 31732 containing 284 amino acid residues, deduced amino acid sequence has 62% identity with those of the HOPDA hydrolases of Pseudomonas putida KF715, P. pseudoalcaligenes KF707, and Burkholderia cepacia LB400, and also significant homology with those of other hydrolytic enzymes including esterase, transferase, and peptidase Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.8 expression in Escherichia coli HB101 Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.8 Pseudomonas sp.
-
 P20
-
3.7.1.8 Pseudomonas sp. P20
-
 P20
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.8 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 Pseudomonas sp. benzoate + 2-hydroxypenta-2,4-dienoate
-
 ?
3.7.1.8 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 Pseudomonas sp. P20 benzoate + 2-hydroxypenta-2,4-dienoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.7.1.8 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
-
 Pseudomonas sp.
3.7.1.8 HOPDA hydrolase
-
 Pseudomonas sp.