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Literature summary extracted from

  • Yu, Z.; Lansdon, E.B.; Segel, I.H.; Fisher, A.J.
    Crystal structure of the bifunctional ATP sulfurylase-APS kinase from the chemolithotrophic thermophile Aquifex aeolicus (2007), J. Mol. Biol., 365, 732-743.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.4 Aquifex aeolicus ATP-sulfurylase-APS kinase is expressed in an Escherichia coli pET vector expression system Aquifex aeolicus
2.7.7.4 expressed in Escherichia coli Aquifex aeolicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.25 crystals are grown by hanging drop vapor diffusion at 25°C in 40% (v/v) ethanol, 1.0% (w/v) PEG 6000, 50 mM sodium acetate and 5 mM MgATP Aquifex aeolicus
2.7.7.4 crystals are grown by hanging drop vapor diffusion at 25°C in 40% (v/v) ethanol, 1% (w/v) PEG 6000, 50 mM sodium acetate and 5 mM MgATP, X-ray crystal structure analysis shows that the protein dimerizes through its APS kinase domain and contains ADP bound in all four active sites Aquifex aeolicus
2.7.7.4 hanging drop vapour diffusion method, at 25 °C, in 40% (v/v) ethanol, 1.0% (w/v) PEG 6000, 50 mM sodium acetate and 5 mM Mg-ATP Aquifex aeolicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.4 Mg2+ required for activity Aquifex aeolicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Aquifex aeolicus O67174
-
-
2.7.7.4 Aquifex aeolicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.25
-
 Aquifex aeolicus
2.7.7.4 hydroxyapatite column chromatography, DEAE cellulose column chromatography, and gel filtration Aquifex aeolicus
2.7.7.4 protein is purified by sequential chromatographies on hydroxyapatite, DEAE cellulose and gel filtration Aquifex aeolicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.4 0.013
-
 purified enzyme has an activity of 0.013-0.016 micromol/min/mg Aquifex aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 ATP + sulfate
-
 Aquifex aeolicus diphosphate + adenylylsulfate
-
 r
2.7.7.4 MgATP2- + adenylyl sulfate reaction carried out by the APS kinase activity of the bifunctional enzyme Aquifex aeolicus MgADP- + 3-phosphoadenylyl sulfate
-
 r
2.7.7.4 MgATP2- + sulfate reaction carried out by the ATP sulfurylase activity of the bifunctional enzyme Aquifex aeolicus magnesium diphosphate + adenylyl sulfate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.4 homodimer x-ray crystallography Aquifex aeolicus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.25 ATP sulfurylase-APS kinase bifunctional enzyme Aquifex aeolicus
2.7.7.4 ATP sulfurylase-APS kinase
-
 Aquifex aeolicus
2.7.7.4 ATP sulfurylase-APS kinase Aquifex aeolicus expresses a gene product that exhibits both ATP sulfurylase and adenosine-5'-phosphosulfate kinase activities Aquifex aeolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.4 additional information
-
 the thermal stability of the Aquifex aeolicus ATP-sulfurylase-APS kinase can be explained by the 43% decreased cavity volume found within the protein core Aquifex aeolicus