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Literature summary extracted from

  • Bains, J.; Boulanger, M.J.
    Biochemical and structural characterization of the paralogous benzoate CoA ligases from Burkholderia xenovorans LB400: defining the entry point into the novel benzoate oxidation (box) pathway (2007), J. Mol. Biol., 373, 965-977.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.25 expressed in Escherichia coli strain BL21 Star (DE3) Paraburkholderia xenovorans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.2.1.25 sitting-drop vapor diffusion method at 18 °C in 23% PEG 3350 K and 0.2 M sodium formate Paraburkholderia xenovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.25 0.12
-
 benzoate in Tris-HCl at pH 8.0 Paraburkholderia xenovorans
6.2.1.25 0.18
-
 CoA in Tris-HCl at pH 8.0 Paraburkholderia xenovorans
6.2.1.25 0.25
-
 2-Aminobenzoate in Tris-HCl at pH 8.0 Paraburkholderia xenovorans
6.2.1.25 0.27
-
 ATP in Tris-HCl at pH 8.0 Paraburkholderia xenovorans

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.25 Paraburkholderia xenovorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.25 Ni-NTA resin column chromatography Paraburkholderia xenovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.25 ATP + 2-aminobenzoate + CoA 12.7% activity compared to benzoate Paraburkholderia xenovorans AMP + diphosphate + 2-aminobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 2-fluorobenzoate + CoA 1.9% activity compared to benzoate Paraburkholderia xenovorans AMP + diphosphate + 2-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 3-fluorobenzoate + CoA 2.0% activity compared to benzoate Paraburkholderia xenovorans AMP + diphosphate + 3-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 3-hydroxybenzoate + CoA 1.1% activity compared to benzoate Paraburkholderia xenovorans AMP + diphosphate + 3-hydroxybenzoyl-CoA
-
 ?
6.2.1.25 ATP + 4-fluorobenzoate + CoA 1.4% activity compared to benzoate Paraburkholderia xenovorans AMP + diphosphate + 4-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 4-hydroxybenzoate + CoA 1.0% activity compared to benzoate Paraburkholderia xenovorans AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
6.2.1.25 ATP + benzoate + CoA 100% activity Paraburkholderia xenovorans AMP + diphosphate + benzoyl-CoA
-
 ?
6.2.1.25 additional information no or less than 1% activity with 2-methylbenzoate, 4-methylbenzoate, 3-chlorobenzoate, 4-chlorobenzoate, 2-bromobenzoate, 3-bromobenzoate, 4-bromobenzoate, phenylacetate, acetate, succinate, propionate, butyrate, and hexanoate Paraburkholderia xenovorans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.25 BCLM
-
 Paraburkholderia xenovorans
6.2.1.25 benzoate CoA ligase
-
 Paraburkholderia xenovorans

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.25 ATP
-
 Paraburkholderia xenovorans