EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.22 | additional information | the enzyme is inducible by cyclohexanone | Acinetobacter calcoaceticus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.22 | expression in Escherichia coli under control of a strong L-arabinose inducible promoter | Acinetobacter calcoaceticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.22 | 0.0014 | - |
bicyclo[3.2.0]hept-2-en-6-one | pH 8.6, 25°C, recombinant and native enzyme | Acinetobacter calcoaceticus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.22 | cyclohexanone + NADPH + O2 | Acinetobacter calcoaceticus | - |
hexano-6-lactone + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.22 | Acinetobacter calcoaceticus | - |
an inducible enzyme | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.22 | recombinant enzyme 21fold from Escherichia coli and native enzyme to homogeneity by ion exchange and affinity chromatography | Acinetobacter calcoaceticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.22 | 9.1 | - |
purified recombinant enzyme, substrate bicyclo[3.2.0]hept-2-en-6-one | Acinetobacter calcoaceticus |
1.14.13.22 | 9.2 | - |
purified native enzyme, substrate bicyclo[3.2.0]hept-2-en-6-one | Acinetobacter calcoaceticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.22 | bicyclo[3.2.0]hept-2-en-6-one + NADPH + O2 | - |
Acinetobacter calcoaceticus | ? | - |
? | |
1.14.13.22 | cyclohexanone + NADPH + O2 | - |
Acinetobacter calcoaceticus | hexano-6-lactone + NADP+ + H2O | - |
? | |
1.14.13.22 | additional information | the enzyme catalyses the NADPH-dependent enantioselective oxidation of ketones and of several heteroatoms such as nitrogen, sulfur, phosphorous and selenium present in organic compounds | Acinetobacter calcoaceticus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.22 | ? | x * 61586.04, recombinant enzyme, mass spectrometry, x * 61583.54, native enzyme, mass spectrometry | Acinetobacter calcoaceticus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.22 | CHMO | - |
Acinetobacter calcoaceticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.22 | 25 | - |
assay at | Acinetobacter calcoaceticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.22 | 8.6 | - |
assay at | Acinetobacter calcoaceticus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.22 | additional information | - |
comparison of pH stability of crude, partially purified, and purified native and recombinant enzymes, overview | Acinetobacter calcoaceticus |
1.14.13.22 | 6 | - |
48 h, purified recombinant enzyme: loss of 12% activity, native enzyme: loss of 24% activity | Acinetobacter calcoaceticus |
1.14.13.22 | 8.6 | - |
48 h, purified recombinant enzyme: loss of 15% activity, native enzyme: loss of 24% activity | Acinetobacter calcoaceticus |
1.14.13.22 | 10 | - |
48 h, purified recombinant enzyme: loss of 45% activity, native enzyme: loss of 82% activity | Acinetobacter calcoaceticus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.22 | FAD | flavoenzyme | Acinetobacter calcoaceticus | |
1.14.13.22 | NADPH | dependent on | Acinetobacter calcoaceticus |