EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.19.3 | expressed in Saccharomyces cerevisiae, strain DBY746 with and without supplementation with linoleic acid | Arthrospira platensis |
1.14.19.46 | expressed in Saccharomyces cerevisiae strain DBY746 | Arthrospira platensis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.19.3 | H129G | expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity, can be rescued by 150 mM exogenous imidazole | Arthrospira platensis |
1.14.19.3 | H129R | expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity using linoleic acid as substrate | Arthrospira platensis |
1.14.19.3 | H305G | expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity, can be rescued by 150 mM exogenous imidazole | Arthrospira platensis |
1.14.19.3 | H305R | expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity using linoleic acid as substrate | Arthrospira platensis |
1.14.19.3 | H89G | expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, very low enzyme activity, can be rescued by 150 mM exogenous imidazole | Arthrospira platensis |
1.14.19.3 | H89R | expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, very low enzyme activity using linoleic acid as substrate | Arthrospira platensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.46 | 1-linoleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Arthrospira platensis | - |
1-gamma-linolenoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.19.46 | 1-linoleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Arthrospira platensis C1 | - |
1-gamma-linolenoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.3 | Arthrospira platensis | - |
C1 | - |
1.14.19.3 | Arthrospira platensis C1 | - |
C1 | - |
1.14.19.46 | Arthrospira platensis | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.3 | linoleic acid + AH2 + O2 | - |
Arthrospira platensis | gamma-linolenic acid + A + H2O | - |
? | |
1.14.19.3 | linoleic acid + AH2 + O2 | - |
Arthrospira platensis C1 | gamma-linolenic acid + A + H2O | - |
? | |
1.14.19.46 | 1-linoleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Arthrospira platensis | 1-gamma-linolenoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.19.46 | 1-linoleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Arthrospira platensis C1 | 1-gamma-linolenoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.19.46 | DELTA6 desaturase | - |
Arthrospira platensis |
1.14.19.46 | desD | - |
Arthrospira platensis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.46 | Ferredoxin | - |
Arthrospira platensis |