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Literature summary extracted from

  • Kurdrid, P.; Subudhi, S.; Cheevadhanarak, S.; Tanticharoen, M.; Hongsthong, A.
    Effect of two intermediate electron donors, NADPH and FADH(2), on Spirulina DELTA6-desaturase co-expressed with two different immediate electron donors, cytochrome b5 and ferredoxin, in Escherichia coli (2006), Mol. Biol. Rep., 34, 261-266.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.19.46 FADH2 about 50% increase of activity in the presence of FADH2 Arthrospira platensis
1.14.19.46 NADPH about 50% increase of activity in the presence of NADPH Arthrospira platensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.3 expressed in Escherichia coli DH5alpha, co-expressed with cytochrome b5 and ferredoxin, no enzyme activity when expressed in Escherichia coli alone Arthrospira platensis
1.14.19.46 expressed in Escherichia coli DH5alpha cells Arthrospira platensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.3 47000
-
 Western Blot, detection of histidine-tagged DELTA6 desaturase expressed in Escherichia coli using a monoclonal antibody against the histidine-tag Arthrospira platensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.46 1-linoleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Arthrospira platensis
-
 1-gamma-linolenoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.3 Arthrospira platensis
-
-
-
1.14.19.46 Arthrospira platensis
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.19.3 additional information
-
 0.3 mM exogenously added NADPH to Escherichia coli cells co-expressing DELTA6 desaturase and cytochrome b5 leads to an increase of the level of gamma-linoleic acid product by 50%, but no effect in Escherichia coli co-expressing DELTA6 desaturase and ferredoxin under the same conditions Arthrospira platensis
1.14.19.3 additional information
-
 co-addition of NADPH and FAD to Escherichia coli cells co-expressing DELTA6 desaturase and ferredoxin leads to an increase of the level of gamma-linoleic acid product Arthrospira platensis
1.14.19.3 additional information
-
 no activity when expressed in Escherichia coli alone, co-expression of cytochrome b5 is needed Arthrospira platensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.3 linoleic acid + AH2 + O2
-
 Arthrospira platensis gamma-linolenic acid + A + H2O
-
 ?
1.14.19.46 1-linoleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Arthrospira platensis 1-gamma-linolenoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.3 DELTA6 desaturase
-
 Arthrospira platensis
1.14.19.46 DELTA6-desaturase
-
 Arthrospira platensis
1.14.19.46 desD
-
 Arthrospira platensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.3 cytochrome b5 with NADPH Arthrospira platensis
1.14.19.3 Ferredoxin with FADH2 Arthrospira platensis
1.14.19.46 Ferredoxin
-
 Arthrospira platensis