Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, F.; Qu, H.; Zhang, D.; Tian, P.; Tan, T.
    Production of 1,3-propanediol from glycerol by recombinant E. coli using incompatible plasmids system (2007), Mol. Biotechnol., 37, 112-119.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.30 ATP
-
 Escherichia coli
4.2.1.30 glycerol dehydratase reactivating factor
-
 Escherichia coli

Application

EC Number Application Comment Organism
4.2.1.30 synthesis synthesis of 1,3-propanediol, which is a suitable monomer for polycondensations to produce polyesters, polyethers, and polyurethanes Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.202 gene dhaT, high level co-expression of the 1,3-PD oxidoreductase with glycerol dehydratase, encoded by gene dhaB, and glycerol dehydratase reactivating factor, encoded by gene gdrAB, in Escherichia coli strain BL21 (DE3) using two incompatible plasmids, fed-batch fermentation of recombinant bacteria. The NADPH-linked alcohol dehydrogenase, which is encoded by yqhD, a gene from Escherichia coli, can non-specifically catalyze 3-hydroxypropionaldehyde converting to 1,3-propanediol with sufficient activity, overview Klebsiella pneumoniae
4.2.1.30 expression in Escherichia coli Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.30 Mg2+ activating Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.202 3-hydroxypropanal + NADH + H+ Klebsiella pneumoniae
-
 propane-1,3-diol + NAD+
-
 r
4.2.1.30 glycerol Escherichia coli
-
 3-hydroxypropionaldehyde + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.202 Klebsiella pneumoniae
-
 strain DSM 2026, gene dhaT
-
4.2.1.30 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.202 3.8
-
 native enzyme Klebsiella pneumoniae
1.1.1.202 9.6
-
 recombinant enzyme Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.202 3-hydroxypropanal + NADH + H+
-
 Klebsiella pneumoniae propane-1,3-diol + NAD+
-
 r
4.2.1.30 glycerol
-
 Escherichia coli 3-hydroxypropionaldehyde + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.202 1,3-PD oxidoreductase
-
 Klebsiella pneumoniae
4.2.1.30 glycerol dehydratase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.202 25
-
 assay at Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.202 NAD+
-
 Klebsiella pneumoniae
1.1.1.202 NADH
-
 Klebsiella pneumoniae
4.2.1.30 coenzyme B12
-
 Escherichia coli