Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Adams, M.A.; Singh, V.K.; Keller, B.O.; Jia, Z.
    Structural and biochemical characterization of gentisate 1,2-dioxygenase from Escherichia coli O157:H7 (2006), Mol. Microbiol., 61, 1469-1484.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.4 expressed in Escherichia coli Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.4 hanging drop vapour diffusion method using 0.1 M BisTris pH 6.5 and 20% polyethylene glycol 5000 monomethylether Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.4 Cu2+ 5 mM, complete inhibition at pH 8.0 Escherichia coli
1.13.11.4 EDTA complete inhibition at pH 8.0 Escherichia coli
1.13.11.4 Li2SO4 5 mM 5-10% inhibition at pH 8.0 Escherichia coli
1.13.11.4 MgSO4 5 mM, 5-10% inhibition at pH 8.0 Escherichia coli
1.13.11.4 Mn2+ 5 mM, complete inhibition at pH 8.0 Escherichia coli
1.13.11.4 additional information no inhibition by 5 mM Ca2+ and K+ Escherichia coli
1.13.11.4 Na2SO4 5 mM, 5-10% inhibition at pH 8.0 Escherichia coli
1.13.11.4 Zn2+ 5 mM, complete inhibition at pH 8.0 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.4 0.011
-
 2,5-Dihydroxybenzoate
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.4 Fe2+ contains 1 mol iron per subunit, in the presence of 2 mM Fe2+ activity is increased by 115% Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.4 38500
-
 4 * 38500, gel filtration Escherichia coli
1.13.11.4 38900
-
 SDS-PAGE Escherichia coli
1.13.11.4 154000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.4 Escherichia coli Q8X655
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.4 nickel-nitriloacetic acid resin column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.4 2,5-dihydroxybenzoate + O2
-
 Escherichia coli maleylpyruvate
-
 ?
1.13.11.4 additional information methyl 2,5-dihydroxybenzoate, 1,4-benzoquinone, 2,5-dihydroxybenzaldehyde, 4,5-dihydroxy-1,3-benzene-disulphonic acid and 2,3,5,6-tetrachloro-1,4-benzenediol give no substrate activity Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.4 tetramer 4 * 38500, gel filtration Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.13.11.4 eGDO
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.4 7.5 8.5
-
 Escherichia coli