Literature summary extracted from

Schjoerring, J.K.; Maeck, G.; Nielsen, K.H.; Husted, S.; Suzuki, A.; Driscoll, S.; Boldt, R.; Bauwe, H.
Antisense reduction of serine hydroxymethyltransferase results in diurnal displacement of NH4+ assimilation in leaves of Solanum tuberosum (2006), Plant J., 45, 71-82.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.1.2.1	full-length cDNA clone SST18 encoding potato SHMT inserted in antisense orientation into the pBIN19 based vector L700 and introduced into the genome of Solanum tuberosum	Solanum tuberosum

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.1.2.1	mitochondrion	-	Solanum tuberosum	5739	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.1.2.1	180000	200000	-	Solanum tuberosum

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.1.2.1	Solanum tuberosum	-	cv. Solara, antisense lines G310-10, G310-16, G310-76, G310-101 and G310-106	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.1.2.1	leaf	SHMT activity of antisense lines is 70-90% lower than in the wild-type, negative effects of antisensed SHMT becomes increasingly severe with plant age	Solanum tuberosum	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.1.2.1	L-serine + tetrahydrofolate	SHMT antisense plants display lower photosynthetic capacity and accumulate glycine in light, glycine is converted to serine in the second half of the light period, serine shows an inverse diurnal rhythm and reaches highest values in darkness, glycine/serine conversion is independent of light in the transformant, but not in the wild-type	Solanum tuberosum	glycine + 5,10-methylenetetrahydrofolate + H2O	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.1.2.1	serine hydroxymethyl transferase	-	Solanum tuberosum
2.1.2.1	SHMT	-	Solanum tuberosum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)