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Literature summary extracted from

  • Kanno, N.; Nagahisa, E.; Sato, M.; Sato, Y.
    Adenosine 5-phosphosulfate sulfotransferase from the marine macroalga Porphyra yezoensis Ueda (Rhodophyta): stabilization, purification, and properties (1996), Planta, 198, 440-446.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.8.2.B1 loss of activity upon freezing and thawing Neopyropia yezoensis
2.8.2.B1 the enzyme is irreversibly inactived by preincubation with thiols Neopyropia yezoensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.B1 Co2+
-
 Neopyropia yezoensis
2.8.2.B1 Cu2+
-
 Neopyropia yezoensis
2.8.2.B1 Fe2+
-
 Neopyropia yezoensis
2.8.2.B1 glutathione slight substrate inhibition Neopyropia yezoensis
2.8.2.B1 Hg2+
-
 Neopyropia yezoensis
2.8.2.B1 Zn2+
-
 Neopyropia yezoensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.B1 0.0021
-
 adenosine 5'-phosphosulfate pH 9.5, 25°C, with dithiothreitol as acceptor substrate Neopyropia yezoensis
2.8.2.B1 0.6
-
 glutathione
-
 Neopyropia yezoensis
2.8.2.B1 0.6
-
 reduced L-glutathione pH 9.5, 25°C Neopyropia yezoensis
2.8.2.B1 1.5
-
 dithiothreitol
-
 Neopyropia yezoensis
2.8.2.B1 1.5
-
 dithioerythritol
-
 Neopyropia yezoensis
2.8.2.B1 1.5
-
 dithiothreitol pH 9.5, 25°C Neopyropia yezoensis
2.8.2.B1 1.5
-
 dithioerythritol pH 9.5, 25°C Neopyropia yezoensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.B1 additional information does not require bivalent cations Neopyropia yezoensis
2.8.2.B1 additional information no requirement for divalent cations. Mg2+, Ca2+, Ba2+ and Mn2+ have no effect Neopyropia yezoensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.B1 43000
-
 x * 43000, SDS-PAGE Neopyropia yezoensis
2.8.2.B1 43000
-
 8 * 43000, SDS-PAGE Neopyropia yezoensis
2.8.2.B1 350000
-
 gel filtration Neopyropia yezoensis

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.B1 Neopyropia yezoensis
-
-
-
2.8.2.B1 Neopyropia yezoensis
-
 Ueda
-
2.8.2.B1 Neopyropia yezoensis Ueda
-
 Ueda
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.B1
-
 Neopyropia yezoensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.B1 thallus
-
 Neopyropia yezoensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.B1 10.3
-
-
 Neopyropia yezoensis

Storage Stability

EC Number Storage Stability Organism
2.8.2.B1 4°C, 50% of the activity is lost within 10 d, when the purified APSSTase is kept under N2 in 0.2M KH2PO4/K/HPO4 buffer (pH 8.0) containing 1.0 M (NH4)2SO4. The half-life for activity loss is slightly extended by the storage in an ice bath (0°C) Neopyropia yezoensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.B1 adenosine 5'-phosphosulfate + dithioerythritol the enzyme is specific for adenosine 5'-phosphosulfate Neopyropia yezoensis ?
-
 ?
2.8.2.B1 adenosine 5'-phosphosulfate + dithioerythritol the enzyme is specific for adenosine 5'-phosphosulfate Neopyropia yezoensis Ueda ?
-
 ?
2.8.2.B1 adenosine 5'-phosphosulfate + dithioerythritol no activity with 3'-phosphoadenosine 5'-phosphosulfate and inosine 5'-phosphosulfate Neopyropia yezoensis AMP + ?
-
 ?
2.8.2.B1 adenosine 5'-phosphosulfate + dithioerythritol no activity with 3'-phosphoadenosine 5'-phosphosulfate and inosine 5'-phosphosulfate Neopyropia yezoensis Ueda AMP + ?
-
 ?
2.8.2.B1 adenosine 5'-phosphosulfate + dithiothreitol the enzyme is specific for adenosine 5'-phosphosulfate Neopyropia yezoensis ?
-
 ?
2.8.2.B1 adenosine 5'-phosphosulfate + dithiothreitol the enzyme is specific for adenosine 5'-phosphosulfate Neopyropia yezoensis Ueda ?
-
 ?
2.8.2.B1 adenosine 5'-phosphosulfate + dithiothreitol no activity with 3'-phosphoadenosine 5'-phosphosulfate and inosine 5'-phosphosulfate Neopyropia yezoensis AMP + ?
-
 ?
2.8.2.B1 adenosine 5'-phosphosulfate + dithiothreitol no activity with 3'-phosphoadenosine 5'-phosphosulfate and inosine 5'-phosphosulfate Neopyropia yezoensis Ueda AMP + ?
-
 ?
2.8.2.B1 adenosine 5'-phosphosulfate + L-glutathione the enzyme is specific for adenosine 5'-phosphosulfate Neopyropia yezoensis ?
-
 ?
2.8.2.B1 adenosine 5'-phosphosulfate + L-glutathione the enzyme is specific for adenosine 5'-phosphosulfate Neopyropia yezoensis Ueda ?
-
 ?
2.8.2.B1 adenosine 5'-phosphosulfate + reduced L-glutathione no activity with 3'-phosphoadenosine 5'-phosphosulfate and inosine 5'-phosphosulfate Neopyropia yezoensis AMP + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.B1 octamer 8 * 43000, SDS-PAGE Neopyropia yezoensis
2.8.2.B1 oligomer x * 43000, SDS-PAGE Neopyropia yezoensis

Synonyms

EC Number Synonyms Comment Organism
2.8.2.B1 APSSTase
-
 Neopyropia yezoensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.2.B1 additional information
-
 thermal inactivation is depressed to a significant extent by a high concentration of Na2SO4. The effect is maximal in the concentration range of 0.4 M-0.6 M. Ammonium sulfate is also effective as an inhibitor of the thermal inactivation. Thermal inactivation is also depressed to some extent by adenosine 5'-phosphosulfate or its analogs, beta-methylene-adenosine 5'-phosphosulfate and AMP. The acceptor substrate dithiothreitol accelerates the thermal inactivation Neopyropia yezoensis
2.8.2.B1 additional information
-
 thermal inactivation is depressed to a significant extent by a high concentration of Na2SO4. The effect is maximal in the concentration range of 0.4-0.6 M. Ammonium sulfate is effective as an inhibitor of the thermal inactivation, but NaC1, CH3COONa or NaNO3 is not. The thermal inactivation is also depressed to some extent by adenosine 5'-phosphosulfate or AMP. The effect is concentration-dependent up to at least 2.0 mM Neopyropia yezoensis
2.8.2.B1 25
-
 pH 8.0, half-life: 8 min Neopyropia yezoensis
2.8.2.B1 25
-
 pH 9.0, about 75% loss of activity after 5 min Neopyropia yezoensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.B1 9 9.8
-
 Neopyropia yezoensis
2.8.2.B1 9.5
-
-
 Neopyropia yezoensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.B1 7 9.5 pH 7.0: about 20% of maximal activity, pH 8.0: 52% of maximal activity, pH 9.5: optimum Neopyropia yezoensis
2.8.2.B1 7 9.8 pH 7.0: about 20% of maximal activity, pH 8.0: 52% of maximal activity, pH 9.0-9.8: optimum Neopyropia yezoensis