EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.3.1.42 | native enzyme and mutants E291L and Y364F. Wild-type enzyme crystallizes as an extraordinary self-inhibiting dimer, dimerization is actively driven by the mutual binding of the two L6 loops into the two active sites | Solanum lycopersicum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.1.42 | E291L | sixfold faster turnover than wild-type. Crystallization in same space group as wild-type, but with strikingly different cell constants. Appears as monomer in the crystal | Solanum lycopersicum |
1.3.1.42 | Y364F | crystallization as a monomer with none of the dimer interactions retained | Solanum lycopersicum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.42 | Solanum lycopersicum | Q9FEW9 | isoform OPR3 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.42 | (9S,13S)-12-oxo-phytodienoic acid + NADPH | - |
Solanum lycopersicum | 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+ | - |
? | |
1.3.1.42 | trans-hex-2-enal + NADPH | - |
Solanum lycopersicum | hexanal + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.42 | dimer | concentration-dependent shift from monomer with 40000 Da at 0.022 mM to dimer with 80000 Da at 0.358 mM, dynamic light scattering | Solanum lycopersicum |
1.3.1.42 | monomer | concentration-dependent shift from monomer with 40000 Da at 0.022 mM to dimer with 80000 Da at 0.358 mM, dynamic light scattering | Solanum lycopersicum |
1.3.1.42 | More | rapid monomer-dimer equilibrium with a high dissociation constant in vitro | Solanum lycopersicum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.42 | additional information | - |
additional information | for oxidative half-reaction, kox of trans-hex-2-2enal is 0.3 per sec | Solanum lycopersicum | |
1.3.1.42 | 14 | - |
trans-hex-2-enal | - |
Solanum lycopersicum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.42 | additional information | in the dimer interface of enzyme, a sulfate ion is bound which forms hydrogen bonds with two arginines of protomer A and one arginine of loop L6 of the partner protomer B. Sulfate ion may mimic the phosphate group of phosphorylated T364 | Solanum lycopersicum |