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Literature summary extracted from

  • Chang, W.N.; Tsai, J.N.; Chen, B.H.; Fu, T.F.
    Cloning, expression, purification, and characterization of zebrafish cytosolic serine hydroxymethyltransferase (2006), Protein Expr. Purif., 46, 212-220.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.2.1 additional information higher concentrations of IPTG, increased induction temperature and/or prolonged induction time increase the ratio of insoluble and soluble SHMT-1 Danio rerio

Application

EC Number Application Comment Organism
2.1.2.1 medicine animal model for drug screening of SHMT Danio rerio

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 into pET43.1a vector and transformed into Escherichia coli HMS174(DE3) cells Danio rerio

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 0.22
-
 L-serine
-
 Danio rerio

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 cytosol
-
 Danio rerio 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 53000
-
 x * 53000, SDS-PAGE, x * 53317, sequence analysis Danio rerio
2.1.2.1 53317
-
 x * 53000, SDS-PAGE, x * 53317, sequence analysis Danio rerio
2.1.2.1 200000
-
 gel filtration, holo- and apoenzyme Danio rerio

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Danio rerio Q2TL58 AB strain
-
2.1.2.1 Danio rerio AB Q2TL58 AB strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 three-step purification, gel filtration, 14.8fold, more than 95% pure Danio rerio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.1 embryo
-
 Danio rerio
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.1 5.46
-
 purified enzyme Danio rerio

Storage Stability

EC Number Storage Stability Organism
2.1.2.1 -20 or -80°C, 10% glycerol, for at least 6 months Danio rerio

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Danio rerio glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
2.1.2.1 L-serine + tetrahydrofolate
-
 Danio rerio AB glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 tetramer x * 53000, SDS-PAGE, x * 53317, sequence analysis Danio rerio

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Danio rerio
2.1.2.1 SHMT-1
-
 Danio rerio

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.2.1 28 37
-
 Danio rerio

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 5.85
-
 L-serine
-
 Danio rerio