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Literature summary extracted from

  • Elias, M.; Dupuy, J.; Merone, L.; Lecomte, C.; Rossi, M.; Masson, P.; Manco, G.; Chabriere, E.
    Crystallization and preliminary X-ray diffraction analysis of the hyperthermophilic Sulfolobus solfataricus phosphotriesterase (2007), Acta Crystallogr. Sect. F, 63, 553-555.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.8.1 expression in Escherichia coli Saccharolobus solfataricus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.8.1 purified recombinant enzyme, hanging-drop vapour-diffusion technique, 5.8 mg/ml protein in 20 mM HEPES, pH 8.5, 0.2 mM CoCl2 and 0.2 M NaCl, 0.001-0.002 ml of protein and reservoir solutions are mixed and equilibrated against 0.8 ml reservoir solution containing 15-18% w/v PEG 8000 and 50 mM Tris-HCl buffer pH 8.0, 1 week at 4°C, X-ray diffraction structure determination and analysis at 2.54 A resolution Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.1.8.1 Saccharolobus solfataricus
-
-
-
3.1.8.1 Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.8.1 recombinant enzyme from Escherichia coli Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.8.1 additional information a organophosphate-degrading enzyme Saccharolobus solfataricus ?
-
 ?
3.1.8.1 additional information a organophosphate-degrading enzyme Saccharolobus solfataricus MT-4 / DSM 5833 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.8.1 phosphotriesterase
-
 Saccharolobus solfataricus
3.1.8.1 PTE
-
 Saccharolobus solfataricus
3.1.8.1 SsoPox
-
 Saccharolobus solfataricus