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Literature summary extracted from

  • Gocke, D.; Nguyen, C.L.; Pohl, M.; Stillger, T.; Walter, L.; Mueller, M.
    Branched-chain keto acid decarboxylase from Lactococcus lactis (KdcA), a valuable thiamine diphosphate-dependent enzyme for asymmetric C-C bond formation (2007), Adv. Synth. Catal., 349, 1425-1435.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.72 expressed in Escherichia coli Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.72 0.127
-
 2-oxo-3-phenylpropanoate in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
4.1.1.72 0.234
-
 Indole-3-pyruvate in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
4.1.1.72 0.264
-
 4-methyl-2-oxopentanoic acid in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
4.1.1.72 0.6
-
 2-oxohexanoic acid in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
4.1.1.72 0.63
-
 3-(4-hydroxyphenyl)-2-oxopropanoic acid in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
4.1.1.72 1.3
-
 2-oxopentanoic acid in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
4.1.1.72 1.3
-
 4-(methylsulfanyl)-2-oxobutanoic acid in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
4.1.1.72 5.02
-
 3-methyl-2-oxobutanoic acid in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
4.1.1.72 7.5
-
 oxo(phenyl)acetic acid in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis
4.1.1.72 29.77
-
 2-Oxopropanoic acid in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.72 73000
-
 2 * 73000, gel filtration Lactococcus lactis
4.1.1.72 126700
-
 calculated from amino acid sequence Lactococcus lactis
4.1.1.72 146000
-
 gel filtration Lactococcus lactis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.1.1.72 DMSO completely stable in the presence of 20% (v/v) DMSO (half-life: 150 h) Lactococcus lactis
4.1.1.72 polyethyleneglycol rapidly inactivated in the presence of 15% (v/v) PEG-400 Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.72 Lactococcus lactis
-
 subspecies Lactococcus lactis cremoris, strain B1157
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.72 immobilized metal chelate chromatography and gel filtration Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.72 2-oxo-3-phenylpropanoic acid at 30 mM 8.6% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis phenylacetaldehyde + CO2
-
 ?
4.1.1.72 2-oxobutanoic acid at 30 mM 9.3% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis propanal + CO2
-
 ?
4.1.1.72 2-oxohexanoic acid at 30 mM 13% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis pentanal + CO2
-
 ?
4.1.1.72 2-oxopentanoic acid at 30 mM 15% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis butanal + CO2
-
 ?
4.1.1.72 2-oxopropanoic acid at 30 mM 1.2% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis acetaldehyde + CO2
-
 ?
4.1.1.72 3-(4-hydroxyphenyl)-2-oxopropanoic acid at 30 mM 6% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis (4-hydroxyphenyl)-acetaldehyde + CO2
-
 ?
4.1.1.72 3-methyl-2-oxobutanoic acid 100% activity Lactococcus lactis 2-methylpropanal + CO2
-
 ?
4.1.1.72 3-methyl-2-oxopentanoic acid at 30 mM 38.1% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis 2-methylbutanal + CO2
-
 ?
4.1.1.72 4-(4-hydroxyphenyl)-2-oxobutanoic acid at 30 mM 1.6% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis 3-(4-hydroxyphenyl)-propanal + CO2
-
 ?
4.1.1.72 4-(methylsulfanyl)-2-oxobutanoic acid at 30 mM 18% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis 3-(methylsulfanyl)-propanal + CO2
-
 ?
4.1.1.72 4-methyl-2-oxohexanoic acid at 30 mM 19% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis 3-methylpentanal + CO2
-
 ?
4.1.1.72 4-methyl-2-oxopentanoic acid at 30 mM 26.3% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis 3-methylbutanal + CO2
-
 ?
4.1.1.72 5-(4-hydroxyphenyl)-2-oxopentanoic acid at 30 mM 1.1% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis 4-(4-hydroxyphenyl)-butanal + CO2
-
 ?
4.1.1.72 benzaldehyde + CO2 self-carboligation Lactococcus lactis (R)-benzoin
-
 ?
4.1.1.72 indole-3-pyruvate at 1 mM 0.85% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis ?
-
 ?
4.1.1.72 isovaleraldehyde self-carboligation Lactococcus lactis ?
-
 ?
4.1.1.72 additional information 3-fluoro-2-oxopropanoic acid, isobutyraldehyde, 3,5-dichlorobenzaldehyde, 3,5-dimethoxybenzaldehyde and 2-oxooctanoic acid are no substrates Lactococcus lactis ?
-
 ?
4.1.1.72 oxo(phenyl)acetic acid at 30 mM 8.4% activity relative to 3-methyl-2-oxobutanoic acid Lactococcus lactis benzaldehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.72 homodimer 2 * 73000, gel filtration Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
4.1.1.72 branched-chain keto acid decarboxylase
-
 Lactococcus lactis
4.1.1.72 KdcA has decarboxylase and carboligase activities Lactococcus lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.72 50
-
-
 Lactococcus lactis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.72 40
-
 sufficiently stable up to 40°C, but rapidly loses activity at higher temperatures Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.72 6 7
-
 Lactococcus lactis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.72 4
-
 rapid inactivation at pH 4 Lactococcus lactis
4.1.1.72 5 7 stable at pH 5-7 Lactococcus lactis
4.1.1.72 8
-
 inactivation at pH 8 Lactococcus lactis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.72 thiamine diphosphate dependent Lactococcus lactis