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Literature summary extracted from

  • Elend, C.; Schmeisser, C.; Leggewie, C.; Babiak, P.; Carballeira, J.D.; Steele, H.L.; Reymond, J.; Jaeger, K.; Streit, W.R.
    Isolation and biochemical characterization of two novel metagenome-derived esterases (2006), Appl. Environ. Microbiol., 72, 3637-3645.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.1 acetone 15% v/v, 128% of initial activity, 30% v/v, 155% of initial activity unidentified
3.1.1.1 acetonitrile 15% v/v, 110% of initial activity, 30% v/v, 87% of initial activity unidentified
3.1.1.1 dimethylformamide 15% v/v, 128% of initial activity, 30% v/v, 155% of initial activity unidentified
3.1.1.1 EDTA slight activation unidentified soil organism
3.1.1.1 Isopropanol 15% v/v, 155% of initial activity, 30% v/v, 117% of initial activity unidentified
3.1.1.1 methanol 15% v/v, 111% of initial activity, 30% v/v, 130% of initial activity unidentified

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expression in Escherichia coli unidentified
3.1.1.1 expression in Escherichia coli unidentified soil organism

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.1 S61A no catalytic activity at 20°C or 45°C unidentified
3.1.1.1 S65A no catalytic activity at 20°C or 45°C unidentified soil organism

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 Ca2+
-
 unidentified
3.1.1.1 Cu2+
-
 unidentified
3.1.1.1 Cu2+ strong unidentified soil organism
3.1.1.1 EDTA
-
 unidentified
3.1.1.1 Mg2+
-
 unidentified
3.1.1.1 Rb+
-
 unidentified
3.1.1.1 sodium dodecylsulfate 0.1%, no effect during short-term incubation, complete inhibition after 60 min unidentified
3.1.1.1 sodium dodecylsulfate 0.1%, no effect during short-term incubation, complete inhibition after 60 min unidentified soil organism
3.1.1.1 Zn2+
-
 unidentified
3.1.1.1 Zn2+
-
 unidentified soil organism

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.1 Ca2+ slight activation unidentified soil organism
3.1.1.1 Co2+ 33% activation unidentified
3.1.1.1 Mg2+ slight activation unidentified soil organism

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 42000
-
 x * 42000, SDS-PAGE, x * 43300, calculated, His-tagged protein unidentified
3.1.1.1 42100
-
 x * 44000, SDS-PAGE, x * 42100, calculated, His-tagged protein unidentified soil organism
3.1.1.1 43300
-
 x * 42000, SDS-PAGE, x * 43300, calculated, His-tagged protein unidentified
3.1.1.1 44000
-
 x * 44000, SDS-PAGE, x * 42100, calculated, His-tagged protein unidentified soil organism
3.1.1.1 260000
-
 gel filtration unidentified
3.1.1.1 260000
-
 gel filtration unidentified soil organism

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.1 Acetone 15% v/v, 128% of initial activity, 30% v/v, 155% of initial activity unidentified
3.1.1.1 Acetone 15% v/v, 14% of initial activity unidentified soil organism
3.1.1.1 acetonitrile 15% v/v, 0% of initial activity unidentified soil organism
3.1.1.1 acetonitrile 15% v/v, 110% of initial activity, 30% v/v, 87% of initial activity unidentified
3.1.1.1 dimethyl sulfoxide up to 15% v/V, enzyme retains full activity unidentified soil organism
3.1.1.1 dimethyl sulfoxide up to 30% v/V, enzyme retains full activity unidentified
3.1.1.1 dimethylformamide 15% v/v, 128% of initial activity, 30% v/v, 155% of initial activity unidentified
3.1.1.1 dimethylformamide 15% v/v, 34% of initial activity unidentified soil organism
3.1.1.1 isopropanol 15% v/v, 155% of initial activity, 30% v/v, 117% of initial activity unidentified
3.1.1.1 isopropanol 15% v/v, 75% of initial activity unidentified soil organism
3.1.1.1 Methanol 15% v/v, 111% of initial activity, 30% v/v, 130% of initial activity unidentified
3.1.1.1 Methanol 15% v/v, 90% of initial activity unidentified soil organism

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 unidentified
-
 enzyme EstA3, isolated from drinking water genome
-
3.1.1.1 unidentified soil organism
-
 enzyme EstCE1, isolated from soil metagenome
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 recombinant enzyme unidentified
3.1.1.1 recombinant enzyme unidentified soil organism

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 1
-
 substrate p-nitrophenyl octanoate, 45°C, pH 7.0 unidentified soil organism
3.1.1.1 3.6
-
 substrate p-nitrophenyl octanoate, 45°C, pH 7.0 unidentified
3.1.1.1 7.4
-
 substrate p-nitrophenyl caproate, 45°C, pH 7.0 unidentified soil organism
3.1.1.1 13.3
-
 substrate vinyl acetate, 45°C, pH 7.0 unidentified soil organism
3.1.1.1 13.3
-
 substrate vinyl propionate, 45°C, pH 7.0 unidentified soil organism
3.1.1.1 16
-
 substrate vinyl butanoate, 45°C, pH 7.0 unidentified soil organism
3.1.1.1 22.9
-
 substrate p-nitrophenyl valerate, 45°C, pH 7.0 unidentified soil organism
3.1.1.1 26.7
-
 substrate triacetin, 45°C, pH 7.0 unidentified soil organism
3.1.1.1 30.9
-
 substrate p-nitrophenyl decanoate, 45°C, pH 7.0 unidentified soil organism
3.1.1.1 31.1
-
 substrate p-nitrophenyl butanoate, 45°C, pH 7.0 unidentified soil organism
3.1.1.1 33.3
-
 substrate tripropionin, 45°C, pH 7.0 unidentified soil organism
3.1.1.1 54.7
-
 substrate tributyrin, 45°C, pH 7.0 unidentified soil organism
3.1.1.1 107.5
-
 substrate vinyl acetate, 45°C, pH 7.0 unidentified
3.1.1.1 147.5
-
 substrate triacetin, 45°C, pH 7.0 unidentified
3.1.1.1 167.5
-
 substrate tributyrin, 45°C, pH 7.0 unidentified
3.1.1.1 380
-
 substrate vinyl propionate, 45°C, pH 7.0 unidentified
3.1.1.1 387.5
-
 substrate vinyl butanoate, 45°C, pH 7.0 unidentified
3.1.1.1 387.5
-
 substrate vinyl caproate, 45°C, pH 7.0 unidentified
3.1.1.1 387.5
-
 substrate vinyl caprylate, 45°C, pH 7.0 unidentified
3.1.1.1 425.7
-
 substrate p-nitrophenyl caproate, 45°C, pH 7.0 unidentified
3.1.1.1 425.7
-
 substrate p-nitrophenyl valerate, 45°C, pH 7.0 unidentified
3.1.1.1 513.6
-
 substrate p-nitrophenyl butanoate, 45°C, pH 7.0 unidentified

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 (+)-menthylacetate + H2O enzyme is highly enantioselective for (+)-menthylacetate unidentified soil organism (+)-menthol + acetate
-
 ?
3.1.1.1 7-[4-octylcarboxy-(3-hydroxy-3-methyl)-butyloxy]-coumarin + H2O
-
 unidentified ?
-
 ?
3.1.1.1 additional information enzyme does not show beta-lactamase activity unidentified ?
-
 ?
3.1.1.1 additional information enzyme does not show beta-lactamase activity unidentified soil organism ?
-
 ?
3.1.1.1 p-nitrophenyl butanoate + H2O
-
 unidentified p-nitrophenol + butanoate
-
 ?
3.1.1.1 p-nitrophenyl butanoate + H2O
-
 unidentified soil organism p-nitrophenol + butanoate
-
 ?
3.1.1.1 p-nitrophenyl caproate
-
 unidentified p-nitrophenol + caproate
-
 ?
3.1.1.1 p-nitrophenyl caproate
-
 unidentified soil organism p-nitrophenol + caproate
-
 ?
3.1.1.1 p-nitrophenyl decanoate
-
 unidentified soil organism p-nitrophenol + octanoate
-
 ?
3.1.1.1 p-nitrophenyl octanoate
-
 unidentified p-nitrophenol + octanoate
-
 ?
3.1.1.1 p-nitrophenyl octanoate
-
 unidentified soil organism p-nitrophenol + octanoate
-
 ?
3.1.1.1 p-nitrophenyl palmitate + H2O
-
 unidentified p-nitrophenol + palmitate
-
 ?
3.1.1.1 p-nitrophenyl valerate + H2O
-
 unidentified p-nitrophenol + valeric acid
-
 ?
3.1.1.1 p-nitrophenyl valerate + H2O
-
 unidentified soil organism p-nitrophenol + valeric acid
-
 ?
3.1.1.1 triacetin + H2O
-
 unidentified acetic acid + glycerol
-
 ?
3.1.1.1 triacetin + H2O
-
 unidentified soil organism acetic acid + glycerol
-
 ?
3.1.1.1 tributyrin + H2O
-
 unidentified butanoate + glycerol
-
 ?
3.1.1.1 tributyrin + H2O
-
 unidentified soil organism butanoate + glycerol
-
 ?
3.1.1.1 tripropionin + H2O
-
 unidentified propanoic acid + glycerol
-
 ?
3.1.1.1 tripropionin + H2O
-
 unidentified soil organism propanoic acid + glycerol
-
 ?
3.1.1.1 vinyl acetate + H2O
-
 unidentified vinyl alcohol + acetate
-
 ?
3.1.1.1 vinyl acetate + H2O
-
 unidentified soil organism vinyl alcohol + acetate
-
 ?
3.1.1.1 vinyl butanoate + H2O
-
 unidentified vinyl alcohol + butanoate
-
 ?
3.1.1.1 vinyl butanoate + H2O
-
 unidentified soil organism vinyl alcohol + butanoate
-
 ?
3.1.1.1 vinyl caproate + H2O
-
 unidentified vinyl alcohol + caproate
-
 ?
3.1.1.1 vinyl caprylate + H2O
-
 unidentified vinyl alcohol + caprylate
-
 ?
3.1.1.1 vinyl propionate + H2O
-
 unidentified vinyl alcohol + propionate
-
 ?
3.1.1.1 vinyl propionate + H2O
-
 unidentified soil organism vinyl alcohol + propionate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 multimer x * 42000, SDS-PAGE, x * 43300, calculated, His-tagged protein unidentified
3.1.1.1 multimer x * 44000, SDS-PAGE, x * 42100, calculated, His-tagged protein unidentified soil organism

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 47
-
-
 unidentified soil organism
3.1.1.1 50
-
-
 unidentified

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.1 36 51 at least 50% of maximum activity within this range unidentified soil organism
3.1.1.1 38 56 at least 50% of maximum activity within this range unidentified

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 40
-
 60 min, stable unidentified
3.1.1.1 40
-
 60 min, stable unidentified soil organism
3.1.1.1 45
-
 rapid inactivation when incubated for more than 60 min unidentified
3.1.1.1 45
-
 rapid inactivation when incubated for more than 60 min unidentified soil organism

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 9
-
-
 unidentified
3.1.1.1 10
-
-
 unidentified soil organism

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 4
-
 no activity below unidentified
3.1.1.1 5
-
 no activity below unidentified soil organism

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.1 1 2 little loss of catalytic activity after incubation for up to 48 h unidentified
3.1.1.1 1 2 little loss of catalytic activity after incubation for up to 48 h unidentified soil organism