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Literature summary extracted from

  • Mandrich, L.; Pezzullo, M.; Rossi, M.; Manco, G.
    SSoNDELTA and SSoNDELTAlong: two thermostable esterases from the same ORF in the archaeon Sulfolobus solfataricus? (2007), Archaea, 2, 109-115.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.79 expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.79 0.03
-
 p-nitrophenyl hexanoate 50°C, SsoNDELTAlong Saccharolobus solfataricus
3.1.1.79 0.054
-
 p-nitrophenyl hexanoate 50°C, SsoNDELTA Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.79 Saccharolobus solfataricus Q97VU2 SSO2517 can be expressed as SsoNDELTA or SsoNDELTAlong (longer version of SsoNDELTA with an extended N-terminus)
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.79 p-nitrophenyl hexanoate + H2O SsoNDELTAlong, is 15fold more active with the substrate p-nitrophenyl hexanoate than SsoNDELTA Saccharolobus solfataricus p-nitrophenol + hexanoate
-
 ?
3.1.1.79 p-nitrophenyl-dodecanoate + H2O SsoNDELTA shows 4.4fold lower activity than SsoNDELTAlong with p-nitrophenyl-dodecanoate as substrate Saccharolobus solfataricus p-nitrophenol + dodecanoate
-
 ?
3.1.1.79 tributyrylglycerol + H2O SsoNDELTA shows 6old lower activity than SsoNDELTAlong with glyceryl tributyrate as substrate Saccharolobus solfataricus ?
-
 ?
3.1.1.79 tridecanoylglycerol + H2O SsoNDELTA shows 4.7fold higher activity than SsoNDELTAlong with glyceryl tridecanoate as substrate Saccharolobus solfataricus ?
-
 ?
3.1.1.79 trihexanoylglycerol + H2O SsoNDELTA shows 4fold lower activity than SsoNDELTAlong with glyceryl trihexanoate as substrate Saccharolobus solfataricus ?
-
 ?
3.1.1.79 trioctanoylglycerol + H2O SsoNDELTA shows 1.7fold lower activity than SsoNDELTAlong with glyceryl trioctanoate as substrate Saccharolobus solfataricus ?
-
 ?
3.1.1.79 trioleoylglycerol + H2O SsoNDELTA shows 10fold higher activity than SsoNDELTAlong with glyceryl trioleate as substrate Saccharolobus solfataricus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.79 hormone-sensitive lipase
-
 Saccharolobus solfataricus
3.1.1.79 SSO2517
-
 Saccharolobus solfataricus
3.1.1.79 SsoNDELTA
-
 Saccharolobus solfataricus
3.1.1.79 SsoNDELTAlong longer version of SsoNDELTA the protein with an extended N-terminus Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.79 85
-
 substrate: p-nitrophenyl hexanoate, SsoNDELTAlong Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.79 70 90 70°C: substrate: about 75% of maximal activity, 90°C: about 90% of maximal activity, p-nitrophenyl hexanoate, SsoNDELTAlong Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.79 2.5
-
 p-nitrophenyl hexanoate 50°C, SsoNDELTA Saccharolobus solfataricus
3.1.1.79 34.5
-
 p-nitrophenyl hexanoate 50°C, SsoNDELTAlong Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.79 6.5
-
 SsoNDELTAlong, substrate: p-nitrophenyl hexanoate Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.79 4.5 7.5 pH 4.5: 80% of maximal activity, pH 7.5: about 65% of maximal activity, substrate: p-nitrophenyl hexanoate, SsoNDELTAlong Saccharolobus solfataricus